Callus production and analysis of some secondary metabolites in Globularia trichosantha subsp. trichosantha

Hatice ÇÖLGEÇEN; Havva ATAR; Gülnur TOKER; Gençay AKGÜL

Makale detayları

Callus production and analysis of some secondary metabolites in Globularia trichosantha subsp. trichosantha

Yazarlar:

Hatice ÇÖLGEÇEN | Havva ATAR | Gülnur TOKER | Gençay AKGÜL

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Botany 2018 Vol.42 Issue 5, pp.559-567

Belge Tipi:

Makale

Yayın Tarihi:

7/2018

Belge Dili:

Türkçe

Document ID (Org No.):

2000815014

Sıklık:

İki Aylık

eISSN:

1303-6106

pISSN:

1300-008X

Özet:

 

This study describes the production of calli from Globularia trichosantha subsp. trichosantha and the quantitative determination of catalpol, aucubin, and verbascoside in the calli and the plant by HPLC. The seeds of the plant were sterilized and germinated in  Murashige and Skoog (MS) medium without in vitro plant growth regulator. Hypocotyl, cotyledon, first leaf, epicotyl, apical meristem,  and root explants were taken from the 30-day-old aseptic seedlings germinated in vitro. Explants were then transferred to MS media  for callus production together with varying concentrations of plant growth regulators. The best callus production occurred in the  media containing 6 mg L?1 IAA and 0.2 mg L?1 2,4-D + 0.1 mg L?1 BAP. The highest catalpol, aucubin, and verbascoside contents were  determined in the roots (1.277 mg kg?1), stem (0.775 mg kg?1), and roots (0.290 mg kg?1), respectively. The study is important in the sense  that, 1) it is the first tissue culture study on G. trichosantha subsp. trichosantha that provides basic information for callus production;  2) that catalpol, aucubin, and verbascoside were quantitatively determined in calli and plant for the first time; and 3) all the secondary  metabolites studied here were produced in the callus culture in a quantity that is approximately 7 times higher than the amount that  naturally occurs in the plant.

Makale detayları

Callus production and analysis of some secondary metabolites in Globularia trichosantha subsp. trichosantha

Yazarlar: Hatice ÇÖLGEÇEN | Havva ATAR | Gülnur TOKER | Gençay AKGÜL

Yayıncı: TUBITAK

Dergi: Turkish Journal of Botany 2018 Vol.42 Issue 5, pp.559-567

Belge Tipi: Makale

Yayın Tarihi: 7/2018

Belge Dili: Türkçe

Document ID (Org No.): 2000815014

Sıklık: İki Aylık

eISSN: 1303-6106

pISSN: 1300-008X

Sayı

Yazarlar:

Hatice ÇÖLGEÇEN | Havva ATAR | Gülnur TOKER | Gençay AKGÜL

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Botany 2018 Vol.42 Issue 5, pp.559-567

Belge Tipi:

Makale

Yayın Tarihi:

7/2018

Belge Dili:

Türkçe

Document ID (Org No.):

2000815014

Sıklık:

İki Aylık

eISSN:

1303-6106

pISSN:

1300-008X