Comparison of UV-spectrophotometry and high- performance liquid chromatography for determination of chlorpheniramine maleate in tablet in the presence of tartrazine

N.A.

Makale detayları

Comparison of UV-spectrophotometry and high- performance liquid chromatography for determination of chlorpheniramine maleate in tablet in the presence of tartrazine

Yazarlar:

N.A.

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmaceutical Chemistry

Dergi:

Journal of Research in Pharmacy Vol.24 Issue 2, pp.290-296

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001346039

pISSN:

2630-6344

Anahtar kelimeler:

Özet:

 The presence of tartrazine, yellow coloring agent, suspected to interfere with the CPM determination in CPM tablets using direct spectrophotometry or high performance liquid chromatography. Overlap spectra of tartrazine and CPM at an analytical wavelength and peak tailing were the main problem occurs in CPM tablets analysis. The aim of this study was to develop a derivative UV spectrophotometry and a modified HPLC to overcome the problem in CPM tablet analysis. Both validated methods were applied for the determination of CPM content in three registered CPM tablets. As a result, the first derivative spectrophotometry method obtained the δAδλ of tartrazine in matrix tablet was nearly zero at the wavelength of 232 nm and did not interfere with the δAδλ of CPM. The selective mobile phase for separation of CPM from tartrazine using HPLC method was a mixture of phosphate buffer pH 4 and methanol 60:40 vv with a flow rate of 1 mlmin. The CPM separated from tartrazine and other peaks in samples with Rs of 1.5 The linearity, accuracy, and precision of these two methods fulfilled the reference requirement. No significant difference observed between the CPM content in artificial tablets when analyzed using first-derivative spectrophotometry and HPLC method. The concentration of CPM in one registered tablet that had been assayed using spectrophotometry, HPLC and the standard method was not significantly different. As a conclusion first-derivative spectrophotometry and HPLC method were valid for the determination of CPM in a tablet containing tartrazine. However, the first-derivative spectrophotometry method was more efficient than HPLC.

 The presence of tartrazine, yellow coloring agent, suspected to interfere with the CPM determination in CPM tablets using direct spectrophotometry or high performance liquid chromatography. Overlap spectra of tartrazine and CPM at an analytical wavelength and peak tailing were the main problem occurs in CPM tablets analysis. The aim of this study was to develop a derivative UV spectrophotometry and a modified HPLC to overcome the problem in CPM tablet analysis. Both validated methods were applied for the determination of CPM content in three registered CPM tablets. As a result, the first derivative spectrophotometry method obtained the δAδλ of tartrazine in matrix tablet was nearly zero at the wavelength of 232 nm and did not interfere with the δAδλ of CPM. The selective mobile phase for separation of CPM from tartrazine using HPLC method was a mixture of phosphate buffer pH 4 and methanol 60:40 vv with a flow rate of 1 mlmin. The CPM separated from tartrazine and other peaks in samples with Rs of 1.5 The linearity, accuracy, and precision of these two methods fulfilled the reference requirement. No significant difference observed between the CPM content in artificial tablets when analyzed using first-derivative spectrophotometry and HPLC method. The concentration of CPM in one registered tablet that had been assayed using spectrophotometry, HPLC and the standard method was not significantly different. As a conclusion first-derivative spectrophotometry and HPLC method were valid for the determination of CPM in a tablet containing tartrazine. However, the first-derivative spectrophotometry method was more efficient than HPLC.

 The presence of tartrazine, yellow coloring agent, suspected to interfere with the CPM determination in CPM tablets using direct spectrophotometry or high performance liquid chromatography. Overlap spectra of tartrazine and CPM at an analytical wavelength and peak tailing were the main problem occurs in CPM tablets analysis. The aim of this study was to develop a derivative UV spectrophotometry and a modified HPLC to overcome the problem in CPM tablet analysis. Both validated methods were applied for the determination of CPM content in three registered CPM tablets. As a result, the first derivative spectrophotometry method obtained the δAδλ of tartrazine in matrix tablet was nearly zero at the wavelength of 232 nm and did not interfere with the δAδλ of CPM. The selective mobile phase for separation of CPM from tartrazine using HPLC method was a mixture of phosphate buffer pH 4 and methanol 60:40 vv with a flow rate of 1 mlmin. The CPM separated from tartrazine and other peaks in samples with Rs of 1.5 The linearity, accuracy, and precision of these two methods fulfilled the reference requirement. No significant difference observed between the CPM content in artificial tablets when analyzed using first-derivative spectrophotometry and HPLC method. The concentration of CPM in one registered tablet that had been assayed using spectrophotometry, HPLC and the standard method was not significantly different. As a conclusion first-derivative spectrophotometry and HPLC method were valid for the determination of CPM in a tablet containing tartrazine. However, the first-derivative spectrophotometry method was more efficient than HPLC.

 The presence of tartrazine, yellow coloring agent, suspected to interfere with the CPM determination in CPM tablets using direct spectrophotometry or high performance liquid chromatography. Overlap spectra of tartrazine and CPM at an analytical wavelength and peak tailing were the main problem occurs in CPM tablets analysis. The aim of this study was to develop a derivative UV spectrophotometry and a modified HPLC to overcome the problem in CPM tablet analysis. Both validated methods were applied for the determination of CPM content in three registered CPM tablets. As a result, the first derivative spectrophotometry method obtained the δAδλ of tartrazine in matrix tablet was nearly zero at the wavelength of 232 nm and did not interfere with the δAδλ of CPM. The selective mobile phase for separation of CPM from tartrazine using HPLC method was a mixture of phosphate buffer pH 4 and methanol 60:40 vv with a flow rate of 1 mlmin. The CPM separated from tartrazine and other peaks in samples with Rs of 1.5 The linearity, accuracy, and precision of these two methods fulfilled the reference requirement. No significant difference observed between the CPM content in artificial tablets when analyzed using first-derivative spectrophotometry and HPLC method. The concentration of CPM in one registered tablet that had been assayed using spectrophotometry, HPLC and the standard method was not significantly different. As a conclusion first-derivative spectrophotometry and HPLC method were valid for the determination of CPM in a tablet containing tartrazine. However, the first-derivative spectrophotometry method was more efficient than HPLC.

Makale detayları

Comparison of UV-spectrophotometry and high- performance liquid chromatography for determination of chlorpheniramine maleate in tablet in the presence of tartrazine

Yazarlar:

N.A.

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmaceutical Chemistry

Dergi:

Journal of Research in Pharmacy Vol.24 Issue 2, pp.290-296

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001346039

pISSN:

2630-6344

Chlorpheniramine maleate

tartrazine

UV-spectrophotometry

HPLC

method validation

Sayı

Makale detayları

Comparison of UV-spectrophotometry and high- performance liquid chromatography for determination of chlorpheniramine maleate in tablet in the presence of tartrazine

Yazarlar: N.A.

Yayıncı: Marmara University

Ana Başlıklar: Pharmaceutical Chemistry

Dergi: Journal of Research in Pharmacy Vol.24 Issue 2, pp.290-296

Belge Tipi: Makale

Yayın Tarihi: 6/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001346039

pISSN: 2630-6344

Chlorpheniramine maleate

tartrazine

UV-spectrophotometry

HPLC

method validation

Sayı

Yazarlar:

N.A.

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmaceutical Chemistry

Dergi:

Journal of Research in Pharmacy Vol.24 Issue 2, pp.290-296

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001346039

pISSN:

2630-6344