Anti-Angiogenic and Oxidant Effects of Monosodium Glutamate at Different Concentrations in Chorioallantoic Membrane Model

Mehmet Enes Sözen; Hasan Basri Savaş; Özgür Akkaya; Oğuz Karahan

Makale detayları

Anti-Angiogenic and Oxidant Effects of Monosodium Glutamate at Different Concentrations in Chorioallantoic Membrane Model

Yazarlar:

Mehmet Enes Sözen | Hasan Basri Savaş | Özgür Akkaya | Oğuz Karahan

Yayıncı:

Mugla Sitki Kocman University

Ana Başlıklar:

Klinik Tıp Bilimleri

Dergi:

Muğla Sıtkı Koçman Üniversitesi Tıp Dergisi Vol.10 Issue 2, pp.110-114

Belge Tipi:

Makale

Yayın Tarihi:

8/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001293769

pISSN:

2148-8118

Anahtar kelimeler:

Özet:

 Monosodium glutamate MSG is a flavor-enhancing food additive. MSG exposure is rising day by day because of the high commercial food consumption. MSG exposure causes damage to various tissues and organs. The aim of this study is to investigate the effects of MSG on angiogenesis and oxidant-antioxidant balance. Three different concentrations of MSG 10-4 M, 10-5 M, and 10-6 M, control, and the bevacizumab 10-6 M were prepared and placed on the chorioallantoic membrane CAM of the embryos. Albumen was taken from the embryos before and after the experiment. Angiogenesis was investigated through the window that was opened on the eggshell. Angiogenesis was found to be normal in the control and 10-6 M MSG group average score: 0.3. Anti-angiogenic effects were moderate in the 10-5 M MSG group average score: 0.5 and in the 10-4 M MSG group average score: 0.7, and strong in the bevacizumab group average score: 1.1. According to our results, MSG shows anti-angiogenic properties in higher doses. MSG increased oxidative stress. According to the results of our research, it is seen that MSG inhibits angiogenesis in a dose-dependent manner in the CAM model and may cause an increase in oxidative damage by disrupting the oxidant-antioxidant balance. Since no previous study has been found in the literature regarding the effects of MSG on angiogenesis and oxidant-antioxidant balance in the CAM model, we think our results will fill an important gap in the literature.

 Monosodium glutamate MSG is a flavor-enhancing food additive. MSG exposure is rising day by day because of the high commercial food consumption. MSG exposure causes damage to various tissues and organs. The aim of this study is to investigate the effects of MSG on angiogenesis and oxidant-antioxidant balance. Three different concentrations of MSG 10-4 M, 10-5 M, and 10-6 M, control, and the bevacizumab 10-6 M were prepared and placed on the chorioallantoic membrane CAM of the embryos. Albumen was taken from the embryos before and after the experiment. Angiogenesis was investigated through the window that was opened on the eggshell. Angiogenesis was found to be normal in the control and 10-6 M MSG group average score: 0.3. Anti-angiogenic effects were moderate in the 10-5 M MSG group average score: 0.5 and in the 10-4 M MSG group average score: 0.7, and strong in the bevacizumab group average score: 1.1. According to our results, MSG shows anti-angiogenic properties in higher doses. MSG increased oxidative stress. According to the results of our research, it is seen that MSG inhibits angiogenesis in a dose-dependent manner in the CAM model and may cause an increase in oxidative damage by disrupting the oxidant-antioxidant balance. Since no previous study has been found in the literature regarding the effects of MSG on angiogenesis and oxidant-antioxidant balance in the CAM model, we think our results will fill an important gap in the literature.

 Monosodium glutamate MSG is a flavor-enhancing food additive. MSG exposure is rising day by day because of the high commercial food consumption. MSG exposure causes damage to various tissues and organs. The aim of this study is to investigate the effects of MSG on angiogenesis and oxidant-antioxidant balance. Three different concentrations of MSG 10-4 M, 10-5 M, and 10-6 M, control, and the bevacizumab 10-6 M were prepared and placed on the chorioallantoic membrane CAM of the embryos. Albumen was taken from the embryos before and after the experiment. Angiogenesis was investigated through the window that was opened on the eggshell. Angiogenesis was found to be normal in the control and 10-6 M MSG group average score: 0.3. Anti-angiogenic effects were moderate in the 10-5 M MSG group average score: 0.5 and in the 10-4 M MSG group average score: 0.7, and strong in the bevacizumab group average score: 1.1. According to our results, MSG shows anti-angiogenic properties in higher doses. MSG increased oxidative stress. According to the results of our research, it is seen that MSG inhibits angiogenesis in a dose-dependent manner in the CAM model and may cause an increase in oxidative damage by disrupting the oxidant-antioxidant balance. Since no previous study has been found in the literature regarding the effects of MSG on angiogenesis and oxidant-antioxidant balance in the CAM model, we think our results will fill an important gap in the literature.

 Monosodyum glutamat MSG, lezzet artırıcı bir gıda katkı maddesidir. İşlenmiş gıda tüketimindeki yükselişe bağlı olarak MSG maruziyeti her geçen gün artmaktadır. MSG maruziyeti çeşitli doku ve organlara zarar vermektedir. Bu çalışmanın amacı, MSGnin anjiyogenez ve oksidan-antioksidan dengesi üzerindeki etkilerini araştırmaktır. Üç farklı konsantrasyonda MSG 10-4 M, 10-5 M ve 10-6 M, kontrol ve bevasizumab 10-6 M hazırlanıp, embriyoların korioallantoik membranına CAM yerleştirildi. Deneyden önce ve sonra tavuk yumurtalarından sıvı alındı. Yumurta kabuğu üzerinde açılan pencereden anjiyogenez araştırıldı. Kontrol ve 10-6 M MSG grubunda ortalama skor: 0.3 anjiyogenez normal bulundu. Anti-anjiyojenik etkilerin, 10-5 M MSG grubunda ortalama puan: 0.5 ve 10-4 M MSG grubunda ortalama puan: 0.7 orta düzeyde ve bevasizumab grubunda ortalama puan: 1.1 güçlü olduğu tespit edildi. Sonuçlarımıza göre MSG daha yüksek dozlarda antianjiyogenik özellikler göstermektedir. Araştırmamızın sonuçlarına göre MSGnin CAM modelinde anjiyogenezi doza bağımlı bir şekilde inhibe ettiği ve oksidan-antioksidan dengesini bozarak oksidatif hasarda artışa neden olabileceği görülmektedir. MSGnin CAM modelinde anjiyogenez ve oksidan-antioksidan denge üzerindeki etkilerine ilişkin literatürde daha önce yapılmış bir çalışma tespit edilemediği için araştırma sonuçlarımızın literatürdeki önemli bir eksikliği gidereceğini düşünmekteyiz.

Makale detayları

Anti-Angiogenic and Oxidant Effects of Monosodium Glutamate at Different Concentrations in Chorioallantoic Membrane Model

Yazarlar:

Mehmet Enes Sözen | Hasan Basri Savaş | Özgür Akkaya | Oğuz Karahan

Yayıncı:

Mugla Sitki Kocman University

Ana Başlıklar:

Klinik Tıp Bilimleri

Dergi:

Muğla Sıtkı Koçman Üniversitesi Tıp Dergisi Vol.10 Issue 2, pp.110-114

Belge Tipi:

Makale

Yayın Tarihi:

8/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001293769

pISSN:

2148-8118

Angiogenesis

Chorioallantoic Membrane Model

Monosodium Glutamate

Oxidative StressAnjiyogenez

Koryoallantoik Membran Modeli

Monosodyum Glutamat

Oksidatif Stres

Sayı

Makale detayları

Yazarlar: Mehmet Enes Sözen | Hasan Basri Savaş | Özgür Akkaya | Oğuz Karahan

Yayıncı: Mugla Sitki Kocman University

Ana Başlıklar: Klinik Tıp Bilimleri

Dergi: Muğla Sıtkı Koçman Üniversitesi Tıp Dergisi Vol.10 Issue 2, pp.110-114

Belge Tipi: Makale

Yayın Tarihi: 8/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001293769

pISSN: 2148-8118

Sayı

Yazarlar:

Mehmet Enes Sözen | Hasan Basri Savaş | Özgür Akkaya | Oğuz Karahan

Yayıncı:

Mugla Sitki Kocman University

Ana Başlıklar:

Klinik Tıp Bilimleri

Dergi:

Muğla Sıtkı Koçman Üniversitesi Tıp Dergisi Vol.10 Issue 2, pp.110-114

Belge Tipi:

Makale

Yayın Tarihi:

8/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001293769

pISSN:

2148-8118