The Effect of Ischemia Reperfusion on Intestinal Contractility Regulated by the Nitrergic System

Fatma ÇAĞLAYAN

Makale detayları

The Effect of Ischemia Reperfusion on Intestinal Contractility Regulated by the Nitrergic System

Yazarlar:

Fatma ÇAĞLAYAN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2004 Vol.28 Issue 5, pp.845-850

Belge Tipi:

Makale

Yayın Tarihi:

4/2004

Belge Dili:

Türkçe

Document ID (Org No.):

2000769369

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

Anahtar kelimeler:

Özet:

 

Because of the interaction between nitric oxide (NO) and the superoxide anion radical, the effect of ischemia-reperfusion on intestinal contractility regulated by the nitrergic system was investigated in the present study.  The study was performed on 3 groups of rabbits: group 1, ischemia; group 2, ischemia and 1 h of reperfusion; group 3, ischemia and 24 h of reperfusion. Tissue samples were obtained from ischemic, ischemia-reperfused and adjacent uninjured intestines as study and control samples. The effects of atropine, tetrodotoxin, L-NAME and L-arginine on the intestinal response to electrical field stimulation (EFS) were investigated. Guanethidine was used to minimize adrenergic activity.  Tetrodotoxin and atropine prevented contractions. L-NAME enhanced the responses to EFS in all tissue samples except for the study tissue of group 2, and L-arginine reversed this contraction elevation. Group 2 study tissue response was as high as 170% of that of the control tissue in standard Krebs-Henseleit solution, and no change was seen on this level with L-NAME and L-arginine addition.  The effects of tetrodotoxin and atropine revealed that EFS affects via the cholinergic neuronal system. Ischemia reperfusion affects intestinal contractility, especially in the early phases of reperfusion. In the light of the increased response to EFS and insensitivity to L-NAME and L-arginine of the affected tissue during this period it was thought that the nitrergic system is considerably affected by ischemia reperfusion. Excessive production of superoxide anion radicals or reversible inhibition of nitric oxide synthase may be the cause of this.

 

Because of the interaction between nitric oxide (NO) and the superoxide anion radical, the effect of ischemia-reperfusion on intestinal contractility regulated by the nitrergic system was investigated in the present study.  The study was performed on 3 groups of rabbits: group 1, ischemia; group 2, ischemia and 1 h of reperfusion; group 3, ischemia and 24 h of reperfusion. Tissue samples were obtained from ischemic, ischemia-reperfused and adjacent uninjured intestines as study and control samples. The effects of atropine, tetrodotoxin, L-NAME and L-arginine on the intestinal response to electrical field stimulation (EFS) were investigated. Guanethidine was used to minimize adrenergic activity.  Tetrodotoxin and atropine prevented contractions. L-NAME enhanced the responses to EFS in all tissue samples except for the study tissue of group 2, and L-arginine reversed this contraction elevation. Group 2 study tissue response was as high as 170% of that of the control tissue in standard Krebs-Henseleit solution, and no change was seen on this level with L-NAME and L-arginine addition.  The effects of tetrodotoxin and atropine revealed that EFS affects via the cholinergic neuronal system. Ischemia reperfusion affects intestinal contractility, especially in the early phases of reperfusion. In the light of the increased response to EFS and insensitivity to L-NAME and L-arginine of the affected tissue during this period it was thought that the nitrergic system is considerably affected by ischemia reperfusion. Excessive production of superoxide anion radicals or reversible inhibition of nitric oxide synthase may be the cause of this.

Makale detayları

The Effect of Ischemia Reperfusion on Intestinal Contractility Regulated by the Nitrergic System

Yazarlar:

Fatma ÇAĞLAYAN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2004 Vol.28 Issue 5, pp.845-850

Belge Tipi:

Makale

Yayın Tarihi:

4/2004

Belge Dili:

Türkçe

Document ID (Org No.):

2000769369

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

Intestinal contractility

intestinal ischemia reperfusion

L-arginine

L-NAME

nitric oxide

Sayı

Makale detayları

The Effect of Ischemia Reperfusion on Intestinal Contractility Regulated by the Nitrergic System

Yazarlar: Fatma ÇAĞLAYAN

Yayıncı: TUBITAK

Dergi: Turkish Journal of Veterinary and Animal Sciences 2004 Vol.28 Issue 5, pp.845-850

Belge Tipi: Makale

Yayın Tarihi: 4/2004

Belge Dili: Türkçe

Document ID (Org No.): 2000769369

Sıklık: İki Aylık

eISSN: 1303-6181

pISSN: 1300-0128

Intestinal contractility

intestinal ischemia reperfusion

L-arginine

L-NAME

nitric oxide

Sayı

Yazarlar:

Fatma ÇAĞLAYAN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2004 Vol.28 Issue 5, pp.845-850

Belge Tipi:

Makale

Yayın Tarihi:

4/2004

Belge Dili:

Türkçe

Document ID (Org No.):

2000769369

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128