Association of molecular polymorphism in exon 2 of Ovar-DRB1 gene with weight traits in Iranian Makuie sheep breed

Fereshteh ASHRAFI; Karim MARDANI; Ali HASHEMI; Reza DARVISHZADEH; Mohammad FARHADIAN

Makale detayları

Association of molecular polymorphism in exon 2 of Ovar-DRB1 gene with weight traits in Iranian Makuie sheep breed

Yazarlar:

Fereshteh ASHRAFI | Karim MARDANI | Ali HASHEMI | Reza DARVISHZADEH | Mohammad FARHADIAN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2014 Vol.38 Issue 2, pp.126-132

Belge Tipi:

Makale

Yayın Tarihi:

5/2014

Belge Dili:

Türkçe

Document ID (Org No.):

2000766043

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

Anahtar kelimeler:

Özet:

 

Exon 2 of the Ovar-DRB1 gene of 90 Makuie sheep was amplified and analyzed by polymerase chain reaction and restriction fragment length polymorphism techniques. Ten alleles and 18 genotypes were identified. The frequencies of the genotypes were 0.317, 0.1585, 0.0121, 0.0365, 0.0121, 0.0243, 0.0243, 0.1097, 0.0121, 0.0487, 0.0121, 0.0365, 0.0365, 0.0487, 0.0121, 0.0121, 0.0487, and 0.0365 for AA, AB, AE, FF, AM, BO, EO, IO, OM, AP, BP, OP, PP, AQ, OQ, PQ, QQ, and AV, respectively. Allele frequencies were 0.4756, 0.0976, 0.0183, 0.0366, 0.0549, 0.0122, 0.1098, 0.0915, 0.0854, and 0.0183 for A, B, E, F, I, M, O, P, Q, and V, respectively. There was no significant (P => 0.05) deviation from Hardy-Weinberg equilibrium for this locus in Makuie sheep. Relationships between molecular polymorphism in the exon 2 region of Ovar-DRB1 and weight traits were investigated. The results showed that the AB genotype was associated with the average of daily weight gain at 9 months and 12 months (P =< 0.05). No correlation of exon 2 Ovar-DRB1 variants with birth weight and other studied traits was detected. We expect that this gene can act as a candidate gene for genetic improvement of some weight traits in sheep breeding programs.

 

Exon 2 of the Ovar-DRB1 gene of 90 Makuie sheep was amplified and analyzed by polymerase chain reaction and restriction fragment length polymorphism techniques. Ten alleles and 18 genotypes were identified. The frequencies of the genotypes were 0.317, 0.1585, 0.0121, 0.0365, 0.0121, 0.0243, 0.0243, 0.1097, 0.0121, 0.0487, 0.0121, 0.0365, 0.0365, 0.0487, 0.0121, 0.0121, 0.0487, and 0.0365 for AA, AB, AE, FF, AM, BO, EO, IO, OM, AP, BP, OP, PP, AQ, OQ, PQ, QQ, and AV, respectively. Allele frequencies were 0.4756, 0.0976, 0.0183, 0.0366, 0.0549, 0.0122, 0.1098, 0.0915, 0.0854, and 0.0183 for A, B, E, F, I, M, O, P, Q, and V, respectively. There was no significant (P => 0.05) deviation from Hardy-Weinberg equilibrium for this locus in Makuie sheep. Relationships between molecular polymorphism in the exon 2 region of Ovar-DRB1 and weight traits were investigated. The results showed that the AB genotype was associated with the average of daily weight gain at 9 months and 12 months (P =< 0.05). No correlation of exon 2 Ovar-DRB1 variants with birth weight and other studied traits was detected. We expect that this gene can act as a candidate gene for genetic improvement of some weight traits in sheep breeding programs.

Makale detayları

Association of molecular polymorphism in exon 2 of Ovar-DRB1 gene with weight traits in Iranian Makuie sheep breed

Yazarlar:

Fereshteh ASHRAFI | Karim MARDANI | Ali HASHEMI | Reza DARVISHZADEH | Mohammad FARHADIAN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2014 Vol.38 Issue 2, pp.126-132

Belge Tipi:

Makale

Yayın Tarihi:

5/2014

Belge Dili:

Türkçe

Document ID (Org No.):

2000766043

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

Key words: Ovar-DRB1 gene

weight traits

Makuie sheep

polymorphism

Sayı

Makale detayları

Association of molecular polymorphism in exon 2 of Ovar-DRB1 gene with weight traits in Iranian Makuie sheep breed

Yazarlar: Fereshteh ASHRAFI | Karim MARDANI | Ali HASHEMI | Reza DARVISHZADEH | Mohammad FARHADIAN

Yayıncı: TUBITAK

Dergi: Turkish Journal of Veterinary and Animal Sciences 2014 Vol.38 Issue 2, pp.126-132

Belge Tipi: Makale

Yayın Tarihi: 5/2014

Belge Dili: Türkçe

Document ID (Org No.): 2000766043

Sıklık: İki Aylık

eISSN: 1303-6181

pISSN: 1300-0128

Key words: Ovar-DRB1 gene

weight traits

Makuie sheep

polymorphism

Sayı

Yazarlar:

Fereshteh ASHRAFI | Karim MARDANI | Ali HASHEMI | Reza DARVISHZADEH | Mohammad FARHADIAN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2014 Vol.38 Issue 2, pp.126-132

Belge Tipi:

Makale

Yayın Tarihi:

5/2014

Belge Dili:

Türkçe

Document ID (Org No.):

2000766043

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128