Evaluation of Immunogenicity of Pasterurella haemolytica Serotypes in Experimental Models

K. Serdar DİKER; Mehmet AKAN

Makale detayları

Evaluation of Immunogenicity of Pasterurella haemolytica Serotypes in Experimental Models

Yazarlar:

K. Serdar DİKER | Mehmet AKAN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2000 Vol.24 Issue 2, pp.139-144

Belge Tipi:

Makale

Yayın Tarihi:

1/2000

Belge Dili:

Türkçe

Document ID (Org No.):

2000771741

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

Anahtar kelimeler:

Özet:

 

The immunogenicity of Pasteurella haemolyticaserotypes was investigated in an experimental animal model. Rabbits were  immunized with bacterins and leukotoxins of selected serotypes of P. haemolytica,and the IgG level and antileukotoxin activities of  immune sera were measured. The highest IgG levels against A1, A2, A7 and T4 antigens were detected in rabbits receiving polyvalent  combined (bacterin plus leukotoxin) immunogens. Monovalent combined immunogens induced high antibody titers against  homologous serotypes, but low titers against heterologous serotypes. Serotype A1 and A7 were found to be more antigenic than  A2 and T4. The highest antileukotoxin activity in monovalent groups was detected in A1 and A7 antisera. Immune sera were used  for the protection of mice challenged with homologous and heterologous serotypes. Antisera to polyvalent combined immunogens  protected 83-100% of mice against A1, A7 and T4 challenge, and 50% of mice against A2 challenge. The lowest protection (16-  33%) was seen in serotype A6-infected mice, as heterologous challenge. In conclusion, marked differences were found in the  immunogenicity of P. haemolytica serotypes and limited protection was observed against heterologous serotypes.

 

The immunogenicity of Pasteurella haemolyticaserotypes was investigated in an experimental animal model. Rabbits were  immunized with bacterins and leukotoxins of selected serotypes of P. haemolytica,and the IgG level and antileukotoxin activities of  immune sera were measured. The highest IgG levels against A1, A2, A7 and T4 antigens were detected in rabbits receiving polyvalent  combined (bacterin plus leukotoxin) immunogens. Monovalent combined immunogens induced high antibody titers against  homologous serotypes, but low titers against heterologous serotypes. Serotype A1 and A7 were found to be more antigenic than  A2 and T4. The highest antileukotoxin activity in monovalent groups was detected in A1 and A7 antisera. Immune sera were used  for the protection of mice challenged with homologous and heterologous serotypes. Antisera to polyvalent combined immunogens  protected 83-100% of mice against A1, A7 and T4 challenge, and 50% of mice against A2 challenge. The lowest protection (16-  33%) was seen in serotype A6-infected mice, as heterologous challenge. In conclusion, marked differences were found in the  immunogenicity of P. haemolytica serotypes and limited protection was observed against heterologous serotypes.

Makale detayları

Evaluation of Immunogenicity of Pasterurella haemolytica Serotypes in Experimental Models

Yazarlar:

K. Serdar DİKER | Mehmet AKAN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2000 Vol.24 Issue 2, pp.139-144

Belge Tipi:

Makale

Yayın Tarihi:

1/2000

Belge Dili:

Türkçe

Document ID (Org No.):

2000771741

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

Pasteurella haemolytica

serotype

immunity

immunogenicity.

Sayı

Makale detayları

Evaluation of Immunogenicity of Pasterurella haemolytica Serotypes in Experimental Models

Yazarlar: K. Serdar DİKER | Mehmet AKAN

Yayıncı: TUBITAK

Dergi: Turkish Journal of Veterinary and Animal Sciences 2000 Vol.24 Issue 2, pp.139-144

Belge Tipi: Makale

Yayın Tarihi: 1/2000

Belge Dili: Türkçe

Document ID (Org No.): 2000771741

Sıklık: İki Aylık

eISSN: 1303-6181

pISSN: 1300-0128

Pasteurella haemolytica

serotype

immunity

immunogenicity.

Sayı

Yazarlar:

K. Serdar DİKER | Mehmet AKAN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2000 Vol.24 Issue 2, pp.139-144

Belge Tipi:

Makale

Yayın Tarihi:

1/2000

Belge Dili:

Türkçe

Document ID (Org No.):

2000771741

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128