Does Glp-2 have a protective effect on cerebral ischemia/reperfusion model?

Naci TOPALOĞLU; Gülsün MEMİ; Tuncay KANER; Mustafa DENİZ; Önder ŞAHİN; Mustafa GÜVEN; Murat ÇOŞAR

Makale detayları

Does Glp-2 have a protective effect on cerebral ischemia/reperfusion model?

Yazarlar:

Naci TOPALOĞLU | Gülsün MEMİ | Tuncay KANER | Mustafa DENİZ | Önder ŞAHİN | Mustafa GÜVEN | Murat ÇOŞAR

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2015 Vol.45 Issue 3, pp.467-473

Belge Tipi:

Makale

Yayın Tarihi:

8/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000752634

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Anahtar kelimeler:

Özet:

 

Background/aim: To investigate the neuroprotective effects of glucagon-like peptide 2 (Glp-2), which increases cerebral blood flow, on the hippocampal complex after cerebral ischemia/reperfusion (I/R) injury in rats.   Materials and methods: Animals were randomized into 4 groups: sham, I/R + 0.9% NaCl, I/R + pre-Glp-2, and I/R + post-Glp-2. Cerebral ischemia was performed via the occlusion of the bilateral internal carotid artery for 40 min and continued with a reperfusion process. At the end of 6 h of reperfusion, animals were decapitated in all groups and brain tissues were removed. Malondialdehyde (MDA) and natural intracellular antioxidant glutathione (GSH) levels and myeloperoxidase (MPO) activities were measured in the left hippocampal tissue. The right hippocampal tissues of all group members were taken for histopathologic study.   Results: MDA levels and MPO activities increased from Group I to Group II and decreased from Group II to Groups III and IV. On the other hand, GSH levels were not significantly different among the groups. The number of apoptotic hippocampal tissue cells increased from Group I to Group II and decreased from Group II to Groups III and IV.  Conclusion: Our preliminary study revealed that Glp-2 treatment may decrease oxidative damage from I/R in cerebral tissue.

 

Background/aim: To investigate the neuroprotective effects of glucagon-like peptide 2 (Glp-2), which increases cerebral blood flow, on the hippocampal complex after cerebral ischemia/reperfusion (I/R) injury in rats.   Materials and methods: Animals were randomized into 4 groups: sham, I/R + 0.9% NaCl, I/R + pre-Glp-2, and I/R + post-Glp-2. Cerebral ischemia was performed via the occlusion of the bilateral internal carotid artery for 40 min and continued with a reperfusion process. At the end of 6 h of reperfusion, animals were decapitated in all groups and brain tissues were removed. Malondialdehyde (MDA) and natural intracellular antioxidant glutathione (GSH) levels and myeloperoxidase (MPO) activities were measured in the left hippocampal tissue. The right hippocampal tissues of all group members were taken for histopathologic study.   Results: MDA levels and MPO activities increased from Group I to Group II and decreased from Group II to Groups III and IV. On the other hand, GSH levels were not significantly different among the groups. The number of apoptotic hippocampal tissue cells increased from Group I to Group II and decreased from Group II to Groups III and IV.  Conclusion: Our preliminary study revealed that Glp-2 treatment may decrease oxidative damage from I/R in cerebral tissue.

Makale detayları

Does Glp-2 have a protective effect on cerebral ischemia/reperfusion model?

Yazarlar:

Naci TOPALOĞLU | Gülsün MEMİ | Tuncay KANER | Mustafa DENİZ | Önder ŞAHİN | Mustafa GÜVEN | Murat ÇOŞAR

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2015 Vol.45 Issue 3, pp.467-473

Belge Tipi:

Makale

Yayın Tarihi:

8/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000752634

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Reperfusion injury

glucagon-like peptide 2

neuroprotective effect

rats

Sayı

Makale detayları

Does Glp-2 have a protective effect on cerebral ischemia/reperfusion model?

Yazarlar: Naci TOPALOĞLU | Gülsün MEMİ | Tuncay KANER | Mustafa DENİZ | Önder ŞAHİN | Mustafa GÜVEN | Murat ÇOŞAR

Yayıncı: TUBITAK

Dergi: Turkish Journal of Medical Sciences 2015 Vol.45 Issue 3, pp.467-473

Belge Tipi: Makale

Yayın Tarihi: 8/2015

Belge Dili: Türkçe

Document ID (Org No.): 2000752634

Sıklık: İki Aylık

eISSN: 1303-6165

pISSN: 1300-0144

Reperfusion injury

glucagon-like peptide 2

neuroprotective effect

rats

Sayı

Yazarlar:

Naci TOPALOĞLU | Gülsün MEMİ | Tuncay KANER | Mustafa DENİZ | Önder ŞAHİN | Mustafa GÜVEN | Murat ÇOŞAR

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2015 Vol.45 Issue 3, pp.467-473

Belge Tipi:

Makale

Yayın Tarihi:

8/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000752634

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144