Antioxidant, Anti-urease Activities and Genotoxic Effects of Terfezia Claveryi Methanol Extracts on Human Lymphocytes

Turgut TAŞKIN; Tugce YESİL DEVECİOGLU; İsmail ŞENKARDEŞ

doi:https://doi.org/10.5152/clinexphealthsci.2017.651

Makale detayları

Antioxidant, Anti-urease Activities and Genotoxic Effects of Terfezia Claveryi Methanol Extracts on Human Lymphocytes

Yazarlar:

Turgut TAŞKIN | Tugce YESİL DEVECİOGLU | İsmail ŞENKARDEŞ

Yayıncı:

Marmara University

Ana Başlıklar:

Sağlık teşvikleri

Dergi:

Clinical and Experimental Health Sciences 2018 Vol.8 Issue 3, pp.190-195

Belge Tipi:

Makale

Yayın Tarihi:

9/2018

Belge Dili:

İngilizce

Document ID (Org No.):

2000907152

Sıklık:

Aylık

eISSN:

2459-1459

DOI:

https://doi.org/10.5152/clinexphealthsci.2017.651

Özet:

 


Objective: Terfezia claveryi is a dark brown color truffle and has been used

extensively in human diet in Turkey. Therefore; it is an important issue to determine

their biological activities and toxicity. This study aims to investigate the antioxidant and

anti-urease effects of T. claveryi methanol extracts and to evaluate the genotoxicity

of each extract.

Methods: The antioxidant activities of methanol extracts were examined by

2,2-diphenyl-1-picryl-hydrazyl (DPPH), 2,2’-azino-bis(3-ethylbenzothiazoline-6-

sulfonate (ABTS) and ferric reducing/antioxidant power (FRAP) methods. The

amount of total phenolic compounds contained in the extracts was determined using

the Folin-Ciocalteu reagent (FCR) method. Anti-urease activities of methanol extracts

were evaluated by indophenol method. In addition, potential DNA damaging effects of

T. claveryi extracts on human lymphocytes were determined by the alkaline comet

assay.

Results: UM extract exhibited higher DPPH radical scavenging activity than MM

extract [the concentration of the substance which provides the inhibition of 50% of the

radical (IC50):0.77±0.01 mg/mL, 1.17±0.02 mg/mL, respectively, p<0.0001] however,

lower activity than standard (p<0.0001). Both extracts in different concentrations of

T. claveryi caused DNA damage (p<0.0001) compared with solvent control DMSO.

Also, total comet scores was higher in cells which had been incubated with MM

extract (p<0.05). UM extract exhibited higher ferric reducing and anti-urease activity

than MM extract but not found statistically different; also activities of both extract were

found lower than positive controls of each methods (p<0.0001).

Conclusion: T. claveryi methanol extracts induced genotoxicity on lymphocytes and

showed very low antioxidant activity compared to standards. This preliminary study

should be confirmed with further studies and in diferent cell lines.

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Makale detayları

Antioxidant, Anti-urease Activities and Genotoxic Effects of Terfezia Claveryi Methanol Extracts on Human Lymphocytes

Yazarlar: Turgut TAŞKIN | Tugce YESİL DEVECİOGLU | İsmail ŞENKARDEŞ

Yayıncı: Marmara University

Ana Başlıklar: Sağlık teşvikleri

Dergi: Clinical and Experimental Health Sciences 2018 Vol.8 Issue 3, pp.190-195

Belge Tipi: Makale

Yayın Tarihi: 9/2018

Belge Dili: İngilizce

Document ID (Org No.): 2000907152

Sıklık: Aylık

eISSN: 2459-1459

DOI: https://doi.org/10.5152/clinexphealthsci.2017.651

Sayı

Yazarlar:

Turgut TAŞKIN | Tugce YESİL DEVECİOGLU | İsmail ŞENKARDEŞ

Yayıncı:

Marmara University

Ana Başlıklar:

Sağlık teşvikleri

Dergi:

Clinical and Experimental Health Sciences 2018 Vol.8 Issue 3, pp.190-195

Belge Tipi:

Makale

Yayın Tarihi:

9/2018

Belge Dili:

İngilizce

Document ID (Org No.):

2000907152

Sıklık:

Aylık

eISSN:

2459-1459

DOI:

https://doi.org/10.5152/clinexphealthsci.2017.651