Quality assessment of marketed food supplements and herbal tea products of olive leaf in Turkey

Etil Güzelmeriç; Dicle Çevik; Erdem Yeşilada

Makale detayları

Quality assessment of marketed food supplements and herbal tea products of olive leaf in Turkey

Yazarlar:

Etil Güzelmeriç | Dicle Çevik | Erdem Yeşilada

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmacognosy

Dergi:

Journal of Research in Pharmacy Vol.24 Issue 1, pp.159-169

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001346761

pISSN:

2630-6344

Anahtar kelimeler:

Özet:

 In this study, qualitative and quantitative analysis of commercially available food supplements composed of olive leaf extract and herbal tea products containing olive leaf were evaluated by High-Performance Thin-Layer Chromatography HPTLC and a newly developed and validated High-Performance Liquid Chromatographic HPLC method for their quality assessment. In addition, leaves of two varieties of Olea europaea L. var. europaea and var. sylvestris grown in Turkey which were assigned as reference plant materials and their chemical compositions were also comparatively analyzed by HPTLC. Then HPTLC fingerprints of reference plant materials were compared to the marketed olive leaf samples. For quantification of oleuropein and luteolin 7-O-glucoside L7G contents in the samples, a simple and fast HPLC method was developed and validated. Consequently, in water and hydroalcoholic extracts of O. europaea var. europaea leaves, oleuropein contents were found to be 15.89 ww and 15.84 ww, while L7G contents were 0.75 and 1.23, respectively. For the reference materials, oleuropein in O. europaea L. var. sylvestris leaves was found to be 12.77 ww, in water extract and 12.36ww, in hydroalcoholic extract, while the concentration of L7G was 0.51 ww and 0.83 ww in water and hydroalcoholic extracts, respectively. Qualitative analysis of the commercial products revealed that fraud was detected in three of eight olive leaf herbal tea bag brands and two of ten olive leaf food supplements. These samples were found either devoid of oleuropein or they had different HPTLC fingerprint profiles than the reference samples.

 In this study, qualitative and quantitative analysis of commercially available food supplements composed of olive leaf extract and herbal tea products containing olive leaf were evaluated by High-Performance Thin-Layer Chromatography HPTLC and a newly developed and validated High-Performance Liquid Chromatographic HPLC method for their quality assessment. In addition, leaves of two varieties of Olea europaea L. var. europaea and var. sylvestris grown in Turkey which were assigned as reference plant materials and their chemical compositions were also comparatively analyzed by HPTLC. Then HPTLC fingerprints of reference plant materials were compared to the marketed olive leaf samples. For quantification of oleuropein and luteolin 7-O-glucoside L7G contents in the samples, a simple and fast HPLC method was developed and validated. Consequently, in water and hydroalcoholic extracts of O. europaea var. europaea leaves, oleuropein contents were found to be 15.89 ww and 15.84 ww, while L7G contents were 0.75 and 1.23, respectively. For the reference materials, oleuropein in O. europaea L. var. sylvestris leaves was found to be 12.77 ww, in water extract and 12.36ww, in hydroalcoholic extract, while the concentration of L7G was 0.51 ww and 0.83 ww in water and hydroalcoholic extracts, respectively. Qualitative analysis of the commercial products revealed that fraud was detected in three of eight olive leaf herbal tea bag brands and two of ten olive leaf food supplements. These samples were found either devoid of oleuropein or they had different HPTLC fingerprint profiles than the reference samples.

 In this study, qualitative and quantitative analysis of commercially available food supplements composed of olive leaf extract and herbal tea products containing olive leaf were evaluated by High-Performance Thin-Layer Chromatography HPTLC and a newly developed and validated High-Performance Liquid Chromatographic HPLC method for their quality assessment. In addition, leaves of two varieties of Olea europaea L. var. europaea and var. sylvestris grown in Turkey which were assigned as reference plant materials and their chemical compositions were also comparatively analyzed by HPTLC. Then HPTLC fingerprints of reference plant materials were compared to the marketed olive leaf samples. For quantification of oleuropein and luteolin 7-O-glucoside L7G contents in the samples, a simple and fast HPLC method was developed and validated. Consequently, in water and hydroalcoholic extracts of O. europaea var. europaea leaves, oleuropein contents were found to be 15.89 ww and 15.84 ww, while L7G contents were 0.75 and 1.23, respectively. For the reference materials, oleuropein in O. europaea L. var. sylvestris leaves was found to be 12.77 ww, in water extract and 12.36ww, in hydroalcoholic extract, while the concentration of L7G was 0.51 ww and 0.83 ww in water and hydroalcoholic extracts, respectively. Qualitative analysis of the commercial products revealed that fraud was detected in three of eight olive leaf herbal tea bag brands and two of ten olive leaf food supplements. These samples were found either devoid of oleuropein or they had different HPTLC fingerprint profiles than the reference samples.

 In this study, qualitative and quantitative analysis of commercially available food supplements composed of olive leaf extract and herbal tea products containing olive leaf were evaluated by High-Performance Thin-Layer Chromatography HPTLC and a newly developed and validated High-Performance Liquid Chromatographic HPLC method for their quality assessment. In addition, leaves of two varieties of Olea europaea L. var. europaea and var. sylvestris grown in Turkey which were assigned as reference plant materials and their chemical compositions were also comparatively analyzed by HPTLC. Then HPTLC fingerprints of reference plant materials were compared to the marketed olive leaf samples. For quantification of oleuropein and luteolin 7-O-glucoside L7G contents in the samples, a simple and fast HPLC method was developed and validated. Consequently, in water and hydroalcoholic extracts of O. europaea var. europaea leaves, oleuropein contents were found to be 15.89 ww and 15.84 ww, while L7G contents were 0.75 and 1.23, respectively. For the reference materials, oleuropein in O. europaea L. var. sylvestris leaves was found to be 12.77 ww, in water extract and 12.36ww, in hydroalcoholic extract, while the concentration of L7G was 0.51 ww and 0.83 ww in water and hydroalcoholic extracts, respectively. Qualitative analysis of the commercial products revealed that fraud was detected in three of eight olive leaf herbal tea bag brands and two of ten olive leaf food supplements. These samples were found either devoid of oleuropein or they had different HPTLC fingerprint profiles than the reference samples.

Makale detayları

Quality assessment of marketed food supplements and herbal tea products of olive leaf in Turkey

Yazarlar:

Etil Güzelmeriç | Dicle Çevik | Erdem Yeşilada

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmacognosy

Dergi:

Journal of Research in Pharmacy Vol.24 Issue 1, pp.159-169

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001346761

pISSN:

2630-6344

Olea europaea

oleuropein

; luteolin 7-O-glucoside

high-performance thin-layer chromatography (HPTLC)

high-performance liquid chromatography (HPLC)

quality control

Sayı

Makale detayları

Quality assessment of marketed food supplements and herbal tea products of olive leaf in Turkey

Yazarlar: Etil Güzelmeriç | Dicle Çevik | Erdem Yeşilada

Yayıncı: Marmara University

Ana Başlıklar: Pharmacognosy

Dergi: Journal of Research in Pharmacy Vol.24 Issue 1, pp.159-169

Belge Tipi: Makale

Yayın Tarihi: 6/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001346761

pISSN: 2630-6344

Olea europaea

oleuropein

; luteolin 7-O-glucoside

high-performance thin-layer chromatography (HPTLC)

high-performance liquid chromatography (HPLC)

quality control

Sayı

Yazarlar:

Etil Güzelmeriç | Dicle Çevik | Erdem Yeşilada

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmacognosy

Dergi:

Journal of Research in Pharmacy Vol.24 Issue 1, pp.159-169

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001346761

pISSN:

2630-6344