Characterization, Antioxidant and Antimicrobial Activities of Iron Nanoparticles Synthesized Using Firethorn Fruit (Pyracantha coccinea Roemer) Extracts

Volkan Karakullukçu; Bülent Akar; Cemallettin Baltacı; Azer Özad Düzgün; Ömer Karpuz

Makale detayları

Characterization, Antioxidant and Antimicrobial Activities of Iron Nanoparticles Synthesized Using Firethorn Fruit (Pyracantha coccinea Roemer) Extracts

Yazarlar:

Volkan Karakullukçu | Bülent Akar | Cemallettin Baltacı | Azer Özad Düzgün | Ömer Karpuz

Yayıncı:

Zonguldak Bulent Ecevit University

Ana Başlıklar:

Mühendislik

Dergi:

Karaelmas Fen ve Mühendislik Dergisi Vol.13 Issue 2, pp.255-265

Belge Tipi:

Makale

Yayın Tarihi:

12/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001307982

pISSN:

2146-7277

Anahtar kelimeler:

Özet:

 The main focus of this study was to explore the biological activity of metal nanoparticles that were green-synthesized from firethorn fruits. Specifically, iron nanoparticles Fe-NP were synthesized from extracts obtained from fruits and seeds of the firethorn plant Pyracantha coccinea grown on Gumushane Universitys campus for landscaping purposes. The antioxidant and antimicrobial properties of both extracts and synthesized iron nanoparticles were evaluated. To characterize the Fe-NPs, FT-IR spectroscopy, scanning electron microscopy SEM, and X-Ray Diffraction XRD analyses were performed. The antioxidant activity of the fruit extracts and Fe-NPs was assessed using various antioxidant activity tests, including ABTS radical scavenging activity, DPPH radical scavenging activity, ferric reducing antioxidant power FRAP, total phenolic content TPC, total flavonoid content TFC, and total antioxidant content TAC. The results of all antioxidant activity tests indicated that the iron nanoparticles exhibited higher antioxidant activity than the fruit extracts. Moreover, the minimum inhibitory concentrations MIC of the fruit extracts and nanoparticles against Gram-negative bacteria Yersinia pseudotuberculosis ATCC 911, Escherichia coli ATCC 25922, Proteus vulgaris ATCC 13315, and Pseudomonas aeruginosa ATCC 43288 and Gram-positive bacteria Streptococcus pyogenes ATCC 19615, Staphylococcus aureus ATCC 25923, and Bacillus subtilis ATCC 6633 were determined the using the medium microdilution procedure. Similar to the antioxidant activity findings, the Fe-NPs demonstrated stronger antimicrobial activity compared to the fruit extracts.

 The main focus of this study was to explore the biological activity of metal nanoparticles that were green-synthesized from firethorn fruits. Specifically, iron nanoparticles Fe-NP were synthesized from extracts obtained from fruits and seeds of the firethorn plant Pyracantha coccinea grown on Gumushane Universitys campus for landscaping purposes. The antioxidant and antimicrobial properties of both extracts and synthesized iron nanoparticles were evaluated. To characterize the Fe-NPs, FT-IR spectroscopy, scanning electron microscopy SEM, and X-Ray Diffraction XRD analyses were performed. The antioxidant activity of the fruit extracts and Fe-NPs was assessed using various antioxidant activity tests, including ABTS radical scavenging activity, DPPH radical scavenging activity, ferric reducing antioxidant power FRAP, total phenolic content TPC, total flavonoid content TFC, and total antioxidant content TAC. The results of all antioxidant activity tests indicated that the iron nanoparticles exhibited higher antioxidant activity than the fruit extracts. Moreover, the minimum inhibitory concentrations MIC of the fruit extracts and nanoparticles against Gram-negative bacteria Yersinia pseudotuberculosis ATCC 911, Escherichia coli ATCC 25922, Proteus vulgaris ATCC 13315, and Pseudomonas aeruginosa ATCC 43288 and Gram-positive bacteria Streptococcus pyogenes ATCC 19615, Staphylococcus aureus ATCC 25923, and Bacillus subtilis ATCC 6633 were determined the using the medium microdilution procedure. Similar to the antioxidant activity findings, the Fe-NPs demonstrated stronger antimicrobial activity compared to the fruit extracts.

 The main focus of this study was to explore the biological activity of metal nanoparticles that were green-synthesized from firethorn fruits. Specifically, iron nanoparticles Fe-NP were synthesized from extracts obtained from fruits and seeds of the firethorn plant Pyracantha coccinea grown on Gumushane Universitys campus for landscaping purposes. The antioxidant and antimicrobial properties of both extracts and synthesized iron nanoparticles were evaluated. To characterize the Fe-NPs, FT-IR spectroscopy, scanning electron microscopy SEM, and X-Ray Diffraction XRD analyses were performed. The antioxidant activity of the fruit extracts and Fe-NPs was assessed using various antioxidant activity tests, including ABTS radical scavenging activity, DPPH radical scavenging activity, ferric reducing antioxidant power FRAP, total phenolic content TPC, total flavonoid content TFC, and total antioxidant content TAC. The results of all antioxidant activity tests indicated that the iron nanoparticles exhibited higher antioxidant activity than the fruit extracts. Moreover, the minimum inhibitory concentrations MIC of the fruit extracts and nanoparticles against Gram-negative bacteria Yersinia pseudotuberculosis ATCC 911, Escherichia coli ATCC 25922, Proteus vulgaris ATCC 13315, and Pseudomonas aeruginosa ATCC 43288 and Gram-positive bacteria Streptococcus pyogenes ATCC 19615, Staphylococcus aureus ATCC 25923, and Bacillus subtilis ATCC 6633 were determined the using the medium microdilution procedure. Similar to the antioxidant activity findings, the Fe-NPs demonstrated stronger antimicrobial activity compared to the fruit extracts.

 Bu çalışmanın amacı, ateş dikeni meyvelerinden yeşil sentez yolu ile elde edilmiş metal nanopartiküllerin biyolojik aktivitesini belirlemektir. Gümüşhane Üniversitesi kampüsünde peyzaj amacıyla yetiştirilen ateş dikeni bitkisinin Pyracantha coccinea meyve ve tohumlarından elde edilen ekstraktlardan demir nanopartikülleri Fe-NP sentezlenmiştir. Hem ekstraktların hem de sentezlenen demir nanopartiküllerinin antioksidan ve antimikrobiyal özellikleri değerlendirilmiştir. Fe-NPleri karakterize etmek için FT-IR spektroskopisi, taramalı elektron mikroskobu SEM ve X-Işını Kırınımı XRD analizleri gerçekleştirilmiştir. Meyve özlerinin ve Fe-NPlerin antioksidan aktivite etkinliği, ABTS radikal süpürme aktivitesi, DPPH radikal süpürme aktivitesi, demir indirgeyici antioksidan güç FRAP, toplam fenolik içerik TPC, toplam flavonoid TFC ve toplam antioksidan içeriği TAC gibi antioksidan aktivite belirleme testleri kullanılarak tespit edilmiştir. Tüm antioksidan aktivite testlerinin sonuçları, demir nanopartiküllerinin meyve ekstraktlarından daha yüksek antioksidan aktivite sergilediğini göstermiştir. Ayrıca Gram-negatif bakterilere Yersinia pseudotuberculosis ATCC 911, Escherichia coli ATCC 25922, Proteus vulgaris ATCC 13315 ve Pseudomonas aeruginosa ATCC 43288 ve Gram-pozitif bakterilere Bacillus subtilis ATCC 6633, Staphylococcus aureus ATCC 25923, Streptococcus pyogenes ATCC 19615 karşı meyve ekstraktlarının ve nanopartiküllerin minimum inhibe edici konsantrasyonu MIK, mikrodilüsyon besiyeri yöntemi kullanılarak belirlenmiştir. Antioksidan aktivite bulgularına benzer şekilde, Fe-NPler meyve özlerine kıyasla daha güçlü antimikrobiyal aktivite göstermiştir.

Makale detayları

Characterization, Antioxidant and Antimicrobial Activities of Iron Nanoparticles Synthesized Using Firethorn Fruit (Pyracantha coccinea Roemer) Extracts

Yazarlar:

Volkan Karakullukçu | Bülent Akar | Cemallettin Baltacı | Azer Özad Düzgün | Ömer Karpuz

Yayıncı:

Zonguldak Bulent Ecevit University

Ana Başlıklar:

Mühendislik

Dergi:

Karaelmas Fen ve Mühendislik Dergisi Vol.13 Issue 2, pp.255-265

Belge Tipi:

Makale

Yayın Tarihi:

12/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001307982

pISSN:

2146-7277

Antimicrobial

Antioxidant

Firethorn

Iron nanoparticle

Pyracantha coccineaAntimikrobiyal

Antioksidan

Ateş dikeni

Demir nanopartikül

Pyracantha coccinea

Sayı

Makale detayları

Yazarlar: Volkan Karakullukçu | Bülent Akar | Cemallettin Baltacı | Azer Özad Düzgün | Ömer Karpuz

Yayıncı: Zonguldak Bulent Ecevit University

Ana Başlıklar: Mühendislik

Dergi: Karaelmas Fen ve Mühendislik Dergisi Vol.13 Issue 2, pp.255-265

Belge Tipi: Makale

Yayın Tarihi: 12/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001307982

pISSN: 2146-7277

Sayı

Yazarlar:

Volkan Karakullukçu | Bülent Akar | Cemallettin Baltacı | Azer Özad Düzgün | Ömer Karpuz

Yayıncı:

Zonguldak Bulent Ecevit University

Ana Başlıklar:

Mühendislik

Dergi:

Karaelmas Fen ve Mühendislik Dergisi Vol.13 Issue 2, pp.255-265

Belge Tipi:

Makale

Yayın Tarihi:

12/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001307982

pISSN:

2146-7277