HPLC fluorescence determination of ochratoxin A utilizing a double internal standard and its application to poultry feed

Muzaffer TUNÇEL; Elif Mine Öncü KAYA; Ülkü Dilek UYSAL; Tufan GÜRAY

Makale detayları

HPLC fluorescence determination of ochratoxin A utilizing a double internal standard and its application to poultry feed

Yazarlar:

Muzaffer TUNÇEL | Elif Mine Öncü KAYA | Ülkü Dilek UYSAL | Tufan GÜRAY

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Chemistry 2015 Vol.39 Issue 2

Belge Tipi:

Makale

Yayın Tarihi:

8/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000726935

Sıklık:

İki Aylık

eISSN:

1303-6130

pISSN:

1300-0527

Özet:

 

A validated liquid chromatography method employing a  fluorescence detector for the determination of ochratoxin A (OTA) was  developed with double internal standard and it was applied to ten different  poultry feeds. The analysis was performed in an octadecyl silane column  using a solvent system [ACN:water:formic acid (50:50:1.25, v/v/v)] by  isocratic elution. The flow rate and injection volume were 1 mL min^{-1}  and 12 \mu L, respectively. Signals were detected at  278(\lambda_{ex})/315(\lambda_{em}) and 330(\lambda_{ex})/450(\lambda_{em}) nm  between 0 and 8, and 8.01 and 20.0 min, respectively. The method was  validated with precision, linearity, accuracy, limit of detection, limit of  quantification, robustness, and stability. Good linearity (r^2 =  0.9998--0.9999) was achieved over a concentration range of 1.60 \times   10^{-8} M to 6.40 \times  10^{-6} M  for OTA. LOD and LOQ values  were 7.83 \times  10^{-10} M and 2.37 \times  10^{-9} M, and  2.01 \times  10^{-9} M and 6.10 \times  10^{-9} M for internal  standard 1 (IS1) and internal standard 2 (IS2), respectively, on an interday  basis. The method was applied to poultry feed samples. Good recovery data  ranged between 79.10% and 85.57%, and 71.98% and 76.66%, and the  RSD% values were in the range of 1.36--11.70 and 2.07--2.34 for IS1 and  IS2, respectively.

 

A validated liquid chromatography method employing a  fluorescence detector for the determination of ochratoxin A (OTA) was  developed with double internal standard and it was applied to ten different  poultry feeds. The analysis was performed in an octadecyl silane column  using a solvent system [ACN:water:formic acid (50:50:1.25, v/v/v)] by  isocratic elution. The flow rate and injection volume were 1 mL min^{-1}  and 12 \mu L, respectively. Signals were detected at  278(\lambda_{ex})/315(\lambda_{em}) and 330(\lambda_{ex})/450(\lambda_{em}) nm  between 0 and 8, and 8.01 and 20.0 min, respectively. The method was  validated with precision, linearity, accuracy, limit of detection, limit of  quantification, robustness, and stability. Good linearity (r^2 =  0.9998--0.9999) was achieved over a concentration range of 1.60 \times   10^{-8} M to 6.40 \times  10^{-6} M  for OTA. LOD and LOQ values  were 7.83 \times  10^{-10} M and 2.37 \times  10^{-9} M, and  2.01 \times  10^{-9} M and 6.10 \times  10^{-9} M for internal  standard 1 (IS1) and internal standard 2 (IS2), respectively, on an interday  basis. The method was applied to poultry feed samples. Good recovery data  ranged between 79.10% and 85.57%, and 71.98% and 76.66%, and the  RSD% values were in the range of 1.36--11.70 and 2.07--2.34 for IS1 and  IS2, respectively.

Makale detayları

HPLC fluorescence determination of ochratoxin A utilizing a double internal standard and its application to poultry feed

Yazarlar: Muzaffer TUNÇEL | Elif Mine Öncü KAYA | Ülkü Dilek UYSAL | Tufan GÜRAY

Yayıncı: TUBITAK

Dergi: Turkish Journal of Chemistry 2015 Vol.39 Issue 2

Belge Tipi: Makale

Yayın Tarihi: 8/2015

Belge Dili: Türkçe

Document ID (Org No.): 2000726935

Sıklık: İki Aylık

eISSN: 1303-6130

pISSN: 1300-0527

Sayı

Yazarlar:

Muzaffer TUNÇEL | Elif Mine Öncü KAYA | Ülkü Dilek UYSAL | Tufan GÜRAY

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Chemistry 2015 Vol.39 Issue 2

Belge Tipi:

Makale

Yayın Tarihi:

8/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000726935

Sıklık:

İki Aylık

eISSN:

1303-6130

pISSN:

1300-0527