Copper bioremoval by novel bacterial isolates and their identification by 16S rRNA gene sequence analysis

Elif ÖLMEZOĞLU; Binnur Kiratli HERAND; Mehmet Salim ÖNCEL; Kenan TUNÇ; Melek ÖZKAN

Makale detayları

Copper bioremoval by novel bacterial isolates and their identification by 16S rRNA gene sequence analysis

Yazarlar:

Elif ÖLMEZOĞLU | Binnur Kiratli HERAND | Mehmet Salim ÖNCEL | Kenan TUNÇ | Melek ÖZKAN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2012 Vol.36 Issue 4, pp.469-476

Belge Tipi:

Makale

Yayın Tarihi:

3/2012

Belge Dili:

Türkçe

Document ID (Org No.):

2000812646

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Anahtar kelimeler:

Özet:

 

Copper-tolerant bacteria were isolated from soil samples taken from a region where metal industries are located. After selecting 2 isolates with relatively higher bioremoval efficiency, the effects of increasing copper concentration, pH, and temperature on the bioremoval efficiency of the growing isolates were determined. Strain N1c and strain N5a showed maximal bioremoval efficiency of 82% and 75%, respectively, in 20 mg/L copper-containing medium at pH 6.8 and 30 °C. Although the isolates did not grow well at pH 5, a low amount of copper was removed at pH 5. Slow growth of N5a at pH 5 allowed for 26% copper removal at hour 80 of incubation. Optimal copper bioremoval of the cells occurred at pH 6.8 and 30 °C. When grown at 37 °C under aerated conditions, N1c showed 31.7% bioremoval in the presence of 100 mg/L copper, and N5a was much more resistant to copper compared to N1c and E. coli. The isolates were identified by 16S rRNA gene sequence analysis. The 16S rRNA gene sequence of N5a showed 96%-97% similarity to Pseudomonas stutzeri and other Pseudomonas spp. The 16S rRNA gene sequence of N1c was 96% similar to Achromobacter sp., Alcaligenes sp., and a novel genus, Collimonas.

 

Copper-tolerant bacteria were isolated from soil samples taken from a region where metal industries are located. After selecting 2 isolates with relatively higher bioremoval efficiency, the effects of increasing copper concentration, pH, and temperature on the bioremoval efficiency of the growing isolates were determined. Strain N1c and strain N5a showed maximal bioremoval efficiency of 82% and 75%, respectively, in 20 mg/L copper-containing medium at pH 6.8 and 30 °C. Although the isolates did not grow well at pH 5, a low amount of copper was removed at pH 5. Slow growth of N5a at pH 5 allowed for 26% copper removal at hour 80 of incubation. Optimal copper bioremoval of the cells occurred at pH 6.8 and 30 °C. When grown at 37 °C under aerated conditions, N1c showed 31.7% bioremoval in the presence of 100 mg/L copper, and N5a was much more resistant to copper compared to N1c and E. coli. The isolates were identified by 16S rRNA gene sequence analysis. The 16S rRNA gene sequence of N5a showed 96%-97% similarity to Pseudomonas stutzeri and other Pseudomonas spp. The 16S rRNA gene sequence of N1c was 96% similar to Achromobacter sp., Alcaligenes sp., and a novel genus, Collimonas.

Makale detayları

Copper bioremoval by novel bacterial isolates and their identification by 16S rRNA gene sequence analysis

Yazarlar:

Elif ÖLMEZOĞLU | Binnur Kiratli HERAND | Mehmet Salim ÖNCEL | Kenan TUNÇ | Melek ÖZKAN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2012 Vol.36 Issue 4, pp.469-476

Belge Tipi:

Makale

Yayın Tarihi:

3/2012

Belge Dili:

Türkçe

Document ID (Org No.):

2000812646

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Key words: Heavy metal resistant bacteria

copper bioremoval

16S rRNA

Sayı

Makale detayları

Copper bioremoval by novel bacterial isolates and their identification by 16S rRNA gene sequence analysis

Yazarlar: Elif ÖLMEZOĞLU | Binnur Kiratli HERAND | Mehmet Salim ÖNCEL | Kenan TUNÇ | Melek ÖZKAN

Yayıncı: TUBITAK

Dergi: Turkish Journal of Biology 2012 Vol.36 Issue 4, pp.469-476

Belge Tipi: Makale

Yayın Tarihi: 3/2012

Belge Dili: Türkçe

Document ID (Org No.): 2000812646

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

Key words: Heavy metal resistant bacteria

copper bioremoval

16S rRNA

Sayı

Yazarlar:

Elif ÖLMEZOĞLU | Binnur Kiratli HERAND | Mehmet Salim ÖNCEL | Kenan TUNÇ | Melek ÖZKAN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2012 Vol.36 Issue 4, pp.469-476

Belge Tipi:

Makale

Yayın Tarihi:

3/2012

Belge Dili:

Türkçe

Document ID (Org No.):

2000812646

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152