Regeneration and Histological Analysis of Regeneration in Bottle Gourd (Lagenaria siceraria (Molina) Stand.)

Yeşim YALÇIN MENDİ; Muzaffer İPEK; Nihal BUZKAN; Yıldız AKA KAÇAR; Sebahattin ÇÜRÜK

Makale detayları

Regeneration and Histological Analysis of Regeneration in Bottle Gourd (Lagenaria siceraria (Molina) Stand.)

Yazarlar:

Yeşim YALÇIN MENDİ | Muzaffer İPEK | Nihal BUZKAN | Yıldız AKA KAÇAR | Sebahattin ÇÜRÜK

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Agriculture and Forestry 2009 Vol.33 Issue 2, pp.165-172

Belge Tipi:

Makale

Yayın Tarihi:

1/2009

Belge Dili:

Türkçe

Document ID (Org No.):

2000808502

Sıklık:

İki Aylık

eISSN:

1303-6173

pISSN:

1300-011X

Anahtar kelimeler:

Özet:

 

Two different types of explant (proximal and flamingo-bill) from Emphasis seedlings, a hybrid cultivar of bottle gourd (Lagenaria siceraria (Molina) Stand.), germinated under dark and light conditions were cultured on 9 regeneration MS media containing various combinations of BA (0, 1.0, and 2.0 mg l-1) and IAA (0, 0.25, and 0.5 mg l-1). Comparison of the explant types showed that the flamingo-bill type explant had better shoot formation than did the proximal explant. The MS medium containing 1 mg l-1 of BA was optimal for shoot formation capacity when flamingo-bill type explants germinated in the dark (44%) and light (36%) were used. Histological analysis showed that explant cell division began after 3 days in regeneration medium and formation of primordium was observed in the tissues in culture between days 5 and 7. Differentiation of meristimatic structures was first observed after 9 days and development completed after 9-12 days in the culture.

 

Two different types of explant (proximal and flamingo-bill) from Emphasis seedlings, a hybrid cultivar of bottle gourd (Lagenaria siceraria (Molina) Stand.), germinated under dark and light conditions were cultured on 9 regeneration MS media containing various combinations of BA (0, 1.0, and 2.0 mg l-1) and IAA (0, 0.25, and 0.5 mg l-1). Comparison of the explant types showed that the flamingo-bill type explant had better shoot formation than did the proximal explant. The MS medium containing 1 mg l-1 of BA was optimal for shoot formation capacity when flamingo-bill type explants germinated in the dark (44%) and light (36%) were used. Histological analysis showed that explant cell division began after 3 days in regeneration medium and formation of primordium was observed in the tissues in culture between days 5 and 7. Differentiation of meristimatic structures was first observed after 9 days and development completed after 9-12 days in the culture.

Makale detayları

Regeneration and Histological Analysis of Regeneration in Bottle Gourd (Lagenaria siceraria (Molina) Stand.)

Yazarlar:

Yeşim YALÇIN MENDİ | Muzaffer İPEK | Nihal BUZKAN | Yıldız AKA KAÇAR | Sebahattin ÇÜRÜK

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Agriculture and Forestry 2009 Vol.33 Issue 2, pp.165-172

Belge Tipi:

Makale

Yayın Tarihi:

1/2009

Belge Dili:

Türkçe

Document ID (Org No.):

2000808502

Sıklık:

İki Aylık

eISSN:

1303-6173

pISSN:

1300-011X

Bottle Gourd

Lagenaria siceraria

regeneration

histology

explant type

Sayı

Makale detayları

Regeneration and Histological Analysis of Regeneration in Bottle Gourd (Lagenaria siceraria (Molina) Stand.)

Yazarlar: Yeşim YALÇIN MENDİ | Muzaffer İPEK | Nihal BUZKAN | Yıldız AKA KAÇAR | Sebahattin ÇÜRÜK

Yayıncı: TUBITAK

Dergi: Turkish Journal of Agriculture and Forestry 2009 Vol.33 Issue 2, pp.165-172

Belge Tipi: Makale

Yayın Tarihi: 1/2009

Belge Dili: Türkçe

Document ID (Org No.): 2000808502

Sıklık: İki Aylık

eISSN: 1303-6173

pISSN: 1300-011X

Bottle Gourd

Lagenaria siceraria

regeneration

histology

explant type

Sayı

Yazarlar:

Yeşim YALÇIN MENDİ | Muzaffer İPEK | Nihal BUZKAN | Yıldız AKA KAÇAR | Sebahattin ÇÜRÜK

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Agriculture and Forestry 2009 Vol.33 Issue 2, pp.165-172

Belge Tipi:

Makale

Yayın Tarihi:

1/2009

Belge Dili:

Türkçe

Document ID (Org No.):

2000808502

Sıklık:

İki Aylık

eISSN:

1303-6173

pISSN:

1300-011X