Effects of Methyl Jasmonate on Phenolic Acid Production in in vitro Root Cultures of Gentiana lutea and Gentiana boissieri

İlknur Albayrak; Alper Cessur; Tunahan Demirci; Nilgün Göktürk Baydar

Makale detayları

Effects of Methyl Jasmonate on Phenolic Acid Production in in vitro Root Cultures of Gentiana lutea and Gentiana boissieri

Yazarlar:

İlknur Albayrak | Alper Cessur | Tunahan Demirci | Nilgün Göktürk Baydar

Yayıncı:

Süleyman Demirel University

Ana Başlıklar:

Plant Biotechnology

Dergi:

Süleyman Demirel University Faculty of Arts and Science Journal of Science Vol.20 Issue 1, pp.25-40

Belge Tipi:

Makale

Yayın Tarihi:

5/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001348847

pISSN:

1306-7575

Anahtar kelimeler:

Özet:

 This research examines the impact of varying durations and concentrations of methyl jasmonate MeJA treatments on the phenolic acid production in in vitro roots of Gentiana lutea and Gentiana boissieri. These species are highly valued for their bioactive metabolites in the pharmaceutical and food industries, as well as their ornamental appeal in horticulture. Nevertheless, both species face threats from overharvesting and human-induced pressures. The study utilized root cultures grown in liquid media, followed by MeJA elicitation at concentrations of 0.1, 0.5, and 1.0 mM for durations of 2, 4, 6, 8, and 10 days. Phenolic acids, including gallic acid GA, chlorogenic acid CGA, caffeic acid CA, p-coumaric acid p-QA, o-coumaric acid o-QA, ferulic acid FA, cinnamic acid CNA, and rosmarinic acid RA, were quantified in the harvested roots using HPLC. The results revealed that phenolic acid levels were influenced by both MeJA concentration and application duration. The findings highlight that MeJA, when optimally applied, can enhance phenolic acid production in in vitro root cultures of G. lutea and G. boissieri effectively.

 This research examines the impact of varying durations and concentrations of methyl jasmonate MeJA treatments on the phenolic acid production in in vitro roots of Gentiana lutea and Gentiana boissieri. These species are highly valued for their bioactive metabolites in the pharmaceutical and food industries, as well as their ornamental appeal in horticulture. Nevertheless, both species face threats from overharvesting and human-induced pressures. The study utilized root cultures grown in liquid media, followed by MeJA elicitation at concentrations of 0.1, 0.5, and 1.0 mM for durations of 2, 4, 6, 8, and 10 days. Phenolic acids, including gallic acid GA, chlorogenic acid CGA, caffeic acid CA, p-coumaric acid p-QA, o-coumaric acid o-QA, ferulic acid FA, cinnamic acid CNA, and rosmarinic acid RA, were quantified in the harvested roots using HPLC. The results revealed that phenolic acid levels were influenced by both MeJA concentration and application duration. The findings highlight that MeJA, when optimally applied, can enhance phenolic acid production in in vitro root cultures of G. lutea and G. boissieri effectively.

 This research examines the impact of varying durations and concentrations of methyl jasmonate MeJA treatments on the phenolic acid production in in vitro roots of Gentiana lutea and Gentiana boissieri. These species are highly valued for their bioactive metabolites in the pharmaceutical and food industries, as well as their ornamental appeal in horticulture. Nevertheless, both species face threats from overharvesting and human-induced pressures. The study utilized root cultures grown in liquid media, followed by MeJA elicitation at concentrations of 0.1, 0.5, and 1.0 mM for durations of 2, 4, 6, 8, and 10 days. Phenolic acids, including gallic acid GA, chlorogenic acid CGA, caffeic acid CA, p-coumaric acid p-QA, o-coumaric acid o-QA, ferulic acid FA, cinnamic acid CNA, and rosmarinic acid RA, were quantified in the harvested roots using HPLC. The results revealed that phenolic acid levels were influenced by both MeJA concentration and application duration. The findings highlight that MeJA, when optimally applied, can enhance phenolic acid production in in vitro root cultures of G. lutea and G. boissieri effectively.

 This research examines the impact of varying durations and concentrations of methyl jasmonate MeJA treatments on the phenolic acid production in in vitro roots of Gentiana lutea and Gentiana boissieri. These species are highly valued for their bioactive metabolites in the pharmaceutical and food industries, as well as their ornamental appeal in horticulture. Nevertheless, both species face threats from overharvesting and human-induced pressures. The study utilized root cultures grown in liquid media, followed by MeJA elicitation at concentrations of 0.1, 0.5, and 1.0 mM for durations of 2, 4, 6, 8, and 10 days. Phenolic acids, including gallic acid GA, chlorogenic acid CGA, caffeic acid CA, p-coumaric acid p-QA, o-coumaric acid o-QA, ferulic acid FA, cinnamic acid CNA, and rosmarinic acid RA, were quantified in the harvested roots using HPLC. The results revealed that phenolic acid levels were influenced by both MeJA concentration and application duration. The findings highlight that MeJA, when optimally applied, can enhance phenolic acid production in in vitro root cultures of G. lutea and G. boissieri effectively.

Makale detayları

Effects of Methyl Jasmonate on Phenolic Acid Production in in vitro Root Cultures of Gentiana lutea and Gentiana boissieri

Yazarlar:

İlknur Albayrak | Alper Cessur | Tunahan Demirci | Nilgün Göktürk Baydar

Yayıncı:

Süleyman Demirel University

Ana Başlıklar:

Plant Biotechnology

Dergi:

Süleyman Demirel University Faculty of Arts and Science Journal of Science Vol.20 Issue 1, pp.25-40

Belge Tipi:

Makale

Yayın Tarihi:

5/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001348847

pISSN:

1306-7575

Plant Biotechnology

Secondary Metabolite Production

In vitro Techniques

Antioxidant Activity

HPLC

Sayı

Makale detayları

Effects of Methyl Jasmonate on Phenolic Acid Production in in vitro Root Cultures of Gentiana lutea and Gentiana boissieri

Yazarlar: İlknur Albayrak | Alper Cessur | Tunahan Demirci | Nilgün Göktürk Baydar

Yayıncı: Süleyman Demirel University

Ana Başlıklar: Plant Biotechnology

Dergi: Süleyman Demirel University Faculty of Arts and Science Journal of Science Vol.20 Issue 1, pp.25-40

Belge Tipi: Makale

Yayın Tarihi: 5/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001348847

pISSN: 1306-7575

Plant Biotechnology

Secondary Metabolite Production

In vitro Techniques

Antioxidant Activity

HPLC

Sayı

Yazarlar:

İlknur Albayrak | Alper Cessur | Tunahan Demirci | Nilgün Göktürk Baydar

Yayıncı:

Süleyman Demirel University

Ana Başlıklar:

Plant Biotechnology

Dergi:

Süleyman Demirel University Faculty of Arts and Science Journal of Science Vol.20 Issue 1, pp.25-40

Belge Tipi:

Makale

Yayın Tarihi:

5/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001348847

pISSN:

1306-7575