Gingipain Injection Affects Intestinal OxidantAntioxidant Status and Alkaline Phosphatase in Overfed Zebrafish

Gizem Gündüz; Merih Beler; İsmail Ünal; Derya Cansız; Ebru Emekli Alturfan; Kemal Naci Kose

Makale detayları

Gingipain Injection Affects Intestinal OxidantAntioxidant Status and Alkaline Phosphatase in Overfed Zebrafish

Yazarlar:

Gizem Gündüz | Merih Beler | İsmail Ünal | Derya Cansız | Ebru Emekli Alturfan | Kemal Naci Kose

Yayıncı:

Istanbul University

Ana Başlıklar:

Clinical Sciences (Other)

Dergi:

Experimed Vol.13 Issue 2, pp.80-85

Belge Tipi:

Makale

Yayın Tarihi:

9/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001298771

pISSN:

2667-5846

Anahtar kelimeler:

Özet:

 Objective: Porphyromonas gingivalis P. gingivalis, a major periodontopathogen, is associated with overfeeding disorders, including metabolic syndrome. Gingipains are one of the most powerful endotoxins of P. gingivalis. Our aim was to reveal the effects of gingipain injections on the intestinal oxidant-antioxidant status and alkaline phosphatase ALP activity in overfed zebrafish. Materials and Methods: Four groups of healthy adult zebrafish were placed in random tanks as C: Control n15; GP: Gingipain n15; OF: Overfeeding n15; and OFGP: OverfeedingGingipain n15 groups. At the end of the experiment, levels of intestinal lipid peroxidation LPO and ALP, glutathione S-transferase GST, and catalase CAT activities were evaluated. Results: Intestinal LPO was significantly lower in the GP and OF groups compared to C. Gingipain injection in OF OFGP significantly elevated LPO when compared to C, GP, and OF groups. ALP activities decreased significantly in the GP, OF, and OFGP compared to C. GST activities increased significantly in the GP when compared to C. Decreased GST activities were observed in the OF and OFGP. This decrease was less in OFGP. CAT activities significantly decreased in all groups when compared to C. Conclusion: Our findings demonstrate that gingipain injection alters the ALP activity and intestinal oxidant-antioxidant status in overfed zebrafish.

 Objective: Porphyromonas gingivalis P. gingivalis, a major periodontopathogen, is associated with overfeeding disorders, including metabolic syndrome. Gingipains are one of the most powerful endotoxins of P. gingivalis. Our aim was to reveal the effects of gingipain injections on the intestinal oxidant-antioxidant status and alkaline phosphatase ALP activity in overfed zebrafish. Materials and Methods: Four groups of healthy adult zebrafish were placed in random tanks as C: Control n15; GP: Gingipain n15; OF: Overfeeding n15; and OFGP: OverfeedingGingipain n15 groups. At the end of the experiment, levels of intestinal lipid peroxidation LPO and ALP, glutathione S-transferase GST, and catalase CAT activities were evaluated. Results: Intestinal LPO was significantly lower in the GP and OF groups compared to C. Gingipain injection in OF OFGP significantly elevated LPO when compared to C, GP, and OF groups. ALP activities decreased significantly in the GP, OF, and OFGP compared to C. GST activities increased significantly in the GP when compared to C. Decreased GST activities were observed in the OF and OFGP. This decrease was less in OFGP. CAT activities significantly decreased in all groups when compared to C. Conclusion: Our findings demonstrate that gingipain injection alters the ALP activity and intestinal oxidant-antioxidant status in overfed zebrafish.

 Objective: Porphyromonas gingivalis P. gingivalis, a major periodontopathogen, is associated with overfeeding disorders, including metabolic syndrome. Gingipains are one of the most powerful endotoxins of P. gingivalis. Our aim was to reveal the effects of gingipain injections on the intestinal oxidant-antioxidant status and alkaline phosphatase ALP activity in overfed zebrafish. Materials and Methods: Four groups of healthy adult zebrafish were placed in random tanks as C: Control n15; GP: Gingipain n15; OF: Overfeeding n15; and OFGP: OverfeedingGingipain n15 groups. At the end of the experiment, levels of intestinal lipid peroxidation LPO and ALP, glutathione S-transferase GST, and catalase CAT activities were evaluated. Results: Intestinal LPO was significantly lower in the GP and OF groups compared to C. Gingipain injection in OF OFGP significantly elevated LPO when compared to C, GP, and OF groups. ALP activities decreased significantly in the GP, OF, and OFGP compared to C. GST activities increased significantly in the GP when compared to C. Decreased GST activities were observed in the OF and OFGP. This decrease was less in OFGP. CAT activities significantly decreased in all groups when compared to C. Conclusion: Our findings demonstrate that gingipain injection alters the ALP activity and intestinal oxidant-antioxidant status in overfed zebrafish.

 Objective: Porphyromonas gingivalis P. gingivalis, a major periodontopathogen, is associated with overfeeding disorders, including metabolic syndrome. Gingipains are one of the most powerful endotoxins of P. gingivalis. Our aim was to reveal the effects of gingipain injections on the intestinal oxidant-antioxidant status and alkaline phosphatase ALP activity in overfed zebrafish. Materials and Methods: Four groups of healthy adult zebrafish were placed in random tanks as C: Control n15; GP: Gingipain n15; OF: Overfeeding n15; and OFGP: OverfeedingGingipain n15 groups. At the end of the experiment, levels of intestinal lipid peroxidation LPO and ALP, glutathione S-transferase GST, and catalase CAT activities were evaluated. Results: Intestinal LPO was significantly lower in the GP and OF groups compared to C. Gingipain injection in OF OFGP significantly elevated LPO when compared to C, GP, and OF groups. ALP activities decreased significantly in the GP, OF, and OFGP compared to C. GST activities increased significantly in the GP when compared to C. Decreased GST activities were observed in the OF and OFGP. This decrease was less in OFGP. CAT activities significantly decreased in all groups when compared to C. Conclusion: Our findings demonstrate that gingipain injection alters the ALP activity and intestinal oxidant-antioxidant status in overfed zebrafish.

Makale detayları

Gingipain Injection Affects Intestinal OxidantAntioxidant Status and Alkaline Phosphatase in Overfed Zebrafish

Yazarlar:

Gizem Gündüz | Merih Beler | İsmail Ünal | Derya Cansız | Ebru Emekli Alturfan | Kemal Naci Kose

Yayıncı:

Istanbul University

Ana Başlıklar:

Clinical Sciences (Other)

Dergi:

Experimed Vol.13 Issue 2, pp.80-85

Belge Tipi:

Makale

Yayın Tarihi:

9/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001298771

pISSN:

2667-5846

Gingipain

zebrafish

oxidative stress

Sayı

Makale detayları

Gingipain Injection Affects Intestinal OxidantAntioxidant Status and Alkaline Phosphatase in Overfed Zebrafish

Yazarlar: Gizem Gündüz | Merih Beler | İsmail Ünal | Derya Cansız | Ebru Emekli Alturfan | Kemal Naci Kose

Yayıncı: Istanbul University

Ana Başlıklar: Clinical Sciences (Other)

Dergi: Experimed Vol.13 Issue 2, pp.80-85

Belge Tipi: Makale

Yayın Tarihi: 9/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001298771

pISSN: 2667-5846

Gingipain

zebrafish

oxidative stress

Sayı

Yazarlar:

Gizem Gündüz | Merih Beler | İsmail Ünal | Derya Cansız | Ebru Emekli Alturfan | Kemal Naci Kose

Yayıncı:

Istanbul University

Ana Başlıklar:

Clinical Sciences (Other)

Dergi:

Experimed Vol.13 Issue 2, pp.80-85

Belge Tipi:

Makale

Yayın Tarihi:

9/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001298771

pISSN:

2667-5846