The Role of Graphene and BiodentineTM on Proliferation and Odontoblastic Differentiation of Pulp Stem Cells

Periş Çelikel; Sera Şimşek Derelioğlu; Fatih Şengül; Ufuk Okkay

Makale detayları

The Role of Graphene and BiodentineTM on Proliferation and Odontoblastic Differentiation of Pulp Stem Cells

Yazarlar:

Periş Çelikel | Sera Şimşek Derelioğlu | Fatih Şengül | Ufuk Okkay

Yayıncı:

Ataturk University

Ana Başlıklar:

Diş Hekimliği (Diğer)

Dergi:

Current Research in Dental Sciences Vol.34 Issue 4, pp.291-296

Belge Tipi:

Makale

Yayın Tarihi:

10/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001328136

pISSN:

2822-2555

Anahtar kelimeler:

Özet:

 Objective: The need for new biomaterials with biocompatibility, mechanical, and antimicrobial properties continues in regenerative endodontic clinical applications in dentistry. Biodentine is successfully used in vital pulp therapies and has regenerative effects. Additionally, graphene, which possesses good physicochemical, mechanical, and biological properties, promotes cellular biomineralization and osteogenic differentiation in dentistry, along with its neuroregenerative effect. This study aims to investigate the effects of graphene and Biodentine on cell proliferation, oxidative stress, and odontogenic differentiation in human dental pulp stem cells hDPSCs. Methods: Cryopreserved hDPSCs purchased from American Type Culture Collection ATCC were used in our study Cat No: PT-5025. hDPSCs were seeded into the E-plate wells and subsequently four different doses of graphene 12.5, 25, 50 and 100 µgml and Biodentine 2, 4, 8 and 16 µgml were added. Results of MTT, total antioxidant capacity TAC, total oxidant status TOS and alkaline phosphatase ALP tests were obtained at the end of the 24th hr. 96 hr-real time cell index data were collected with xCELLigence system. Resulting data were compared using one-way analysis of variance ANOVA. Results: 12.5 µgml graphene and 2 µgml Biodentine were found to be the subgroups with the highest levels of cell proliferation and the lowest oxidative stress. Antioxidative effect was determined in all Biodentine doses but only in 12.5 µgml graphene. Odontogenic differentiation was observed in all doses of graphene and Biodentine. Conclusion: 12.5 µgml graphene and 2 µgml Biodentine were observed to have positive impacts on the proliferation, oxidative stress and odontogenic differentiations of hDPSCs. Keywords: Cell proliferation. Graphene. Oxidative stress. Odontogenic differentiation. Tricalcium silicate.

 Objective: The need for new biomaterials with biocompatibility, mechanical, and antimicrobial properties continues in regenerative endodontic clinical applications in dentistry. Biodentine is successfully used in vital pulp therapies and has regenerative effects. Additionally, graphene, which possesses good physicochemical, mechanical, and biological properties, promotes cellular biomineralization and osteogenic differentiation in dentistry, along with its neuroregenerative effect. This study aims to investigate the effects of graphene and Biodentine on cell proliferation, oxidative stress, and odontogenic differentiation in human dental pulp stem cells hDPSCs. Methods: Cryopreserved hDPSCs purchased from American Type Culture Collection ATCC were used in our study Cat No: PT-5025. hDPSCs were seeded into the E-plate wells and subsequently four different doses of graphene 12.5, 25, 50 and 100 µgml and Biodentine 2, 4, 8 and 16 µgml were added. Results of MTT, total antioxidant capacity TAC, total oxidant status TOS and alkaline phosphatase ALP tests were obtained at the end of the 24th hr. 96 hr-real time cell index data were collected with xCELLigence system. Resulting data were compared using one-way analysis of variance ANOVA. Results: 12.5 µgml graphene and 2 µgml Biodentine were found to be the subgroups with the highest levels of cell proliferation and the lowest oxidative stress. Antioxidative effect was determined in all Biodentine doses but only in 12.5 µgml graphene. Odontogenic differentiation was observed in all doses of graphene and Biodentine. Conclusion: 12.5 µgml graphene and 2 µgml Biodentine were observed to have positive impacts on the proliferation, oxidative stress and odontogenic differentiations of hDPSCs. Keywords: Cell proliferation. Graphene. Oxidative stress. Odontogenic differentiation. Tricalcium silicate.

 Objective: The need for new biomaterials with biocompatibility, mechanical, and antimicrobial properties continues in regenerative endodontic clinical applications in dentistry. Biodentine is successfully used in vital pulp therapies and has regenerative effects. Additionally, graphene, which possesses good physicochemical, mechanical, and biological properties, promotes cellular biomineralization and osteogenic differentiation in dentistry, along with its neuroregenerative effect. This study aims to investigate the effects of graphene and Biodentine on cell proliferation, oxidative stress, and odontogenic differentiation in human dental pulp stem cells hDPSCs. Methods: Cryopreserved hDPSCs purchased from American Type Culture Collection ATCC were used in our study Cat No: PT-5025. hDPSCs were seeded into the E-plate wells and subsequently four different doses of graphene 12.5, 25, 50 and 100 µgml and Biodentine 2, 4, 8 and 16 µgml were added. Results of MTT, total antioxidant capacity TAC, total oxidant status TOS and alkaline phosphatase ALP tests were obtained at the end of the 24th hr. 96 hr-real time cell index data were collected with xCELLigence system. Resulting data were compared using one-way analysis of variance ANOVA. Results: 12.5 µgml graphene and 2 µgml Biodentine were found to be the subgroups with the highest levels of cell proliferation and the lowest oxidative stress. Antioxidative effect was determined in all Biodentine doses but only in 12.5 µgml graphene. Odontogenic differentiation was observed in all doses of graphene and Biodentine. Conclusion: 12.5 µgml graphene and 2 µgml Biodentine were observed to have positive impacts on the proliferation, oxidative stress and odontogenic differentiations of hDPSCs. Keywords: Cell proliferation. Graphene. Oxidative stress. Odontogenic differentiation. Tricalcium silicate.

 Objective: The need for new biomaterials with biocompatibility, mechanical, and antimicrobial properties continues in regenerative endodontic clinical applications in dentistry. Biodentine is successfully used in vital pulp therapies and has regenerative effects. Additionally, graphene, which possesses good physicochemical, mechanical, and biological properties, promotes cellular biomineralization and osteogenic differentiation in dentistry, along with its neuroregenerative effect. This study aims to investigate the effects of graphene and Biodentine on cell proliferation, oxidative stress, and odontogenic differentiation in human dental pulp stem cells hDPSCs. Methods: Cryopreserved hDPSCs purchased from American Type Culture Collection ATCC were used in our study Cat No: PT-5025. hDPSCs were seeded into the E-plate wells and subsequently four different doses of graphene 12.5, 25, 50 and 100 µgml and Biodentine 2, 4, 8 and 16 µgml were added. Results of MTT, total antioxidant capacity TAC, total oxidant status TOS and alkaline phosphatase ALP tests were obtained at the end of the 24th hr. 96 hr-real time cell index data were collected with xCELLigence system. Resulting data were compared using one-way analysis of variance ANOVA. Results: 12.5 µgml graphene and 2 µgml Biodentine were found to be the subgroups with the highest levels of cell proliferation and the lowest oxidative stress. Antioxidative effect was determined in all Biodentine doses but only in 12.5 µgml graphene. Odontogenic differentiation was observed in all doses of graphene and Biodentine. Conclusion: 12.5 µgml graphene and 2 µgml Biodentine were observed to have positive impacts on the proliferation, oxidative stress and odontogenic differentiations of hDPSCs. Keywords: Cell proliferation. Graphene. Oxidative stress. Odontogenic differentiation. Tricalcium silicate.

Makale detayları

The Role of Graphene and BiodentineTM on Proliferation and Odontoblastic Differentiation of Pulp Stem Cells

Yazarlar: Periş Çelikel | Sera Şimşek Derelioğlu | Fatih Şengül | Ufuk Okkay

Yayıncı: Ataturk University

Ana Başlıklar: Diş Hekimliği (Diğer)

Dergi: Current Research in Dental Sciences Vol.34 Issue 4, pp.291-296

Belge Tipi: Makale

Yayın Tarihi: 10/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001328136

pISSN: 2822-2555

Cell proliferation

Graphene

Oxidative stress

Odontogenic differentiation

Tricalcium silicate

Sayı

Yazarlar:

Periş Çelikel | Sera Şimşek Derelioğlu | Fatih Şengül | Ufuk Okkay

Yayıncı:

Ataturk University

Ana Başlıklar:

Diş Hekimliği (Diğer)

Dergi:

Current Research in Dental Sciences Vol.34 Issue 4, pp.291-296

Belge Tipi:

Makale

Yayın Tarihi:

10/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001328136

pISSN:

2822-2555