Antiviral and molecular docking analysis of methoxyflavones isolated from Melicope latifolia leaves against HCV

N.A.

Makale detayları

Antiviral and molecular docking analysis of methoxyflavones isolated from Melicope latifolia leaves against HCV

Yazarlar:

N.A.

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmacology and Pharmaceutical Sciences (Other)

Dergi:

Journal of Research in Pharmacy Vol.27 Issue 3, pp.1301-1312

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001347906

pISSN:

2630-6344

Anahtar kelimeler:

Özet:

 Treatments of hepatitis C virus have been developed and increased the sustained virology response SVR, however, there are several reports of drug resistance, high-cost issue, and limited access to current hepatitis C virus HCV treatment that remain become a problem. This necessitates to search for complementary and alternative drugs against HCV, therefore the investigation of active compounds from plant extracts such as Melicope latifolia, a plant that has been reported as anti-HCV, will be provided in this study. The anti-HCV activities were tested using in vitro cultured cells of hepatocyte cell line Huh 7.5 and HCV genotype 2a J6JFH1. Ethanol extract of M. latifolia leaves was separated by chromatographic methods and the chemical structures of the isolated compounds were established based on mass spectrometry, 1D, and 2D nuclear magnetic resonance spectral data, as well as comparison with reported data. The interaction of the compound with the protein, which involves to HCV activity, was determined by docking analysis. Three known methoxyflavone compounds identified as 5,4-dihydroxy-7-prenyloxy-3,8,5-trimethoxyflavone 1; 5,3- dihydroxy-3,7,8,4-tetramethoxyflavone 2; and 5-hydroxy-3,7,8,3,4-pentamethoxyflavone 3 were isolated from the ethanolic extract of M. latifolia leaves. Anti-HCV activities revealed that compound 1 strongly inhibited HCV J6JFH1 with a 50 inhibitory concentration IC50 value of 6.70.4 µgmL and 50 cytotoxic concentration CC50 of 19.3 µgmL. The docking analysis revealed an interaction with the 4GAG, a protein that involves in the entry step of HCV, and the 4EAW protein which plays an important role during HCV replication.

 Treatments of hepatitis C virus have been developed and increased the sustained virology response SVR, however, there are several reports of drug resistance, high-cost issue, and limited access to current hepatitis C virus HCV treatment that remain become a problem. This necessitates to search for complementary and alternative drugs against HCV, therefore the investigation of active compounds from plant extracts such as Melicope latifolia, a plant that has been reported as anti-HCV, will be provided in this study. The anti-HCV activities were tested using in vitro cultured cells of hepatocyte cell line Huh 7.5 and HCV genotype 2a J6JFH1. Ethanol extract of M. latifolia leaves was separated by chromatographic methods and the chemical structures of the isolated compounds were established based on mass spectrometry, 1D, and 2D nuclear magnetic resonance spectral data, as well as comparison with reported data. The interaction of the compound with the protein, which involves to HCV activity, was determined by docking analysis. Three known methoxyflavone compounds identified as 5,4-dihydroxy-7-prenyloxy-3,8,5-trimethoxyflavone 1; 5,3- dihydroxy-3,7,8,4-tetramethoxyflavone 2; and 5-hydroxy-3,7,8,3,4-pentamethoxyflavone 3 were isolated from the ethanolic extract of M. latifolia leaves. Anti-HCV activities revealed that compound 1 strongly inhibited HCV J6JFH1 with a 50 inhibitory concentration IC50 value of 6.70.4 µgmL and 50 cytotoxic concentration CC50 of 19.3 µgmL. The docking analysis revealed an interaction with the 4GAG, a protein that involves in the entry step of HCV, and the 4EAW protein which plays an important role during HCV replication.

 Treatments of hepatitis C virus have been developed and increased the sustained virology response SVR, however, there are several reports of drug resistance, high-cost issue, and limited access to current hepatitis C virus HCV treatment that remain become a problem. This necessitates to search for complementary and alternative drugs against HCV, therefore the investigation of active compounds from plant extracts such as Melicope latifolia, a plant that has been reported as anti-HCV, will be provided in this study. The anti-HCV activities were tested using in vitro cultured cells of hepatocyte cell line Huh 7.5 and HCV genotype 2a J6JFH1. Ethanol extract of M. latifolia leaves was separated by chromatographic methods and the chemical structures of the isolated compounds were established based on mass spectrometry, 1D, and 2D nuclear magnetic resonance spectral data, as well as comparison with reported data. The interaction of the compound with the protein, which involves to HCV activity, was determined by docking analysis. Three known methoxyflavone compounds identified as 5,4-dihydroxy-7-prenyloxy-3,8,5-trimethoxyflavone 1; 5,3- dihydroxy-3,7,8,4-tetramethoxyflavone 2; and 5-hydroxy-3,7,8,3,4-pentamethoxyflavone 3 were isolated from the ethanolic extract of M. latifolia leaves. Anti-HCV activities revealed that compound 1 strongly inhibited HCV J6JFH1 with a 50 inhibitory concentration IC50 value of 6.70.4 µgmL and 50 cytotoxic concentration CC50 of 19.3 µgmL. The docking analysis revealed an interaction with the 4GAG, a protein that involves in the entry step of HCV, and the 4EAW protein which plays an important role during HCV replication.

 Treatments of hepatitis C virus have been developed and increased the sustained virology response SVR, however, there are several reports of drug resistance, high-cost issue, and limited access to current hepatitis C virus HCV treatment that remain become a problem. This necessitates to search for complementary and alternative drugs against HCV, therefore the investigation of active compounds from plant extracts such as Melicope latifolia, a plant that has been reported as anti-HCV, will be provided in this study. The anti-HCV activities were tested using in vitro cultured cells of hepatocyte cell line Huh 7.5 and HCV genotype 2a J6JFH1. Ethanol extract of M. latifolia leaves was separated by chromatographic methods and the chemical structures of the isolated compounds were established based on mass spectrometry, 1D, and 2D nuclear magnetic resonance spectral data, as well as comparison with reported data. The interaction of the compound with the protein, which involves to HCV activity, was determined by docking analysis. Three known methoxyflavone compounds identified as 5,4-dihydroxy-7-prenyloxy-3,8,5-trimethoxyflavone 1; 5,3- dihydroxy-3,7,8,4-tetramethoxyflavone 2; and 5-hydroxy-3,7,8,3,4-pentamethoxyflavone 3 were isolated from the ethanolic extract of M. latifolia leaves. Anti-HCV activities revealed that compound 1 strongly inhibited HCV J6JFH1 with a 50 inhibitory concentration IC50 value of 6.70.4 µgmL and 50 cytotoxic concentration CC50 of 19.3 µgmL. The docking analysis revealed an interaction with the 4GAG, a protein that involves in the entry step of HCV, and the 4EAW protein which plays an important role during HCV replication.

Makale detayları

Antiviral and molecular docking analysis of methoxyflavones isolated from Melicope latifolia leaves against HCV

Yazarlar:

N.A.

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmacology and Pharmaceutical Sciences (Other)

Dergi:

Journal of Research in Pharmacy Vol.27 Issue 3, pp.1301-1312

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001347906

pISSN:

2630-6344

AntiHCV

Infectious disease

Melicope latifolia

medicine

hepatitis

health

Sayı

Makale detayları

Antiviral and molecular docking analysis of methoxyflavones isolated from Melicope latifolia leaves against HCV

Yazarlar: N.A.

Yayıncı: Marmara University

Ana Başlıklar: Pharmacology and Pharmaceutical Sciences (Other)

Dergi: Journal of Research in Pharmacy Vol.27 Issue 3, pp.1301-1312

Belge Tipi: Makale

Yayın Tarihi: 6/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001347906

pISSN: 2630-6344

AntiHCV

Infectious disease

Melicope latifolia

medicine

hepatitis

health

Sayı

Yazarlar:

N.A.

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmacology and Pharmaceutical Sciences (Other)

Dergi:

Journal of Research in Pharmacy Vol.27 Issue 3, pp.1301-1312

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001347906

pISSN:

2630-6344