Partial purification and some properties of aspartate aminotransferase from the mantle tissue of Mytilus galloprovincialis Lam

Özlem DÖNMEZ YURTPINAR; Tuğba YILMAZ ÖZDEN; Ayşe CAN

Makale detayları

Partial purification and some properties of aspartate aminotransferase from the mantle tissue of Mytilus galloprovincialis Lam

Yazarlar:

Özlem DÖNMEZ YURTPINAR | Tuğba YILMAZ ÖZDEN | Ayşe CAN

Yayıncı:

Istanbul University

Ana Başlıklar:

Farmakoloji ve terapötik

Dergi:

Journal of Faculty of Pharmacy of Istanbul University 2014 Vol.44 Issue 1, pp.1-10

Belge Tipi:

Makale

Yayın Tarihi:

1/2015

Belge Dili:

İngilizce

Document ID (Org No.):

2000513200

Sıklık:

Aylık

eISSN:

2587-2087

Özet:

 

Aspartate aminotransferase (E.C.2.6.1.1; AST), is a pyridoxal phosphate dependent enzyme that occurs in virtually all organisms and plays a key role in intermediary nitrogen metabolism. Although AST was purified from a variety of plant and animal sources, it has not been purified previously from mantle tissue of Mytilus galloprovincialis Lam. In the present study we have partially purified AST from the mantle tissue of M. galloprovincialis and investigated some kinetic properties of the enzyme. The partially purified enzyme showed three protein and a single activity band in polyacrylamide gel electrophoresis. It was found that the enzyme exhibited maximum activity at 15ºC and 35ºC and that the activity was decreased at 40ºC and totally lost at 55ºC. AST activity was maximum at pH 7.4 in Tris-HCl buffer. Km values of AST for aspartate and 2-oxoglutarate were 1.64 mM and 2.2x10-2 mM, respectively, and Vmax values for the same substrates were 0.12 U/mL and 0.168 U/mL, respectively.

Makale detayları

Partial purification and some properties of aspartate aminotransferase from the mantle tissue of Mytilus galloprovincialis Lam

Yazarlar: Özlem DÖNMEZ YURTPINAR | Tuğba YILMAZ ÖZDEN | Ayşe CAN

Yayıncı: Istanbul University

Ana Başlıklar: Farmakoloji ve terapötik

Dergi: Journal of Faculty of Pharmacy of Istanbul University 2014 Vol.44 Issue 1, pp.1-10

Belge Tipi: Makale

Yayın Tarihi: 1/2015

Belge Dili: İngilizce

Document ID (Org No.): 2000513200

Sıklık: Aylık

eISSN: 2587-2087

Sayı

Yazarlar:

Özlem DÖNMEZ YURTPINAR | Tuğba YILMAZ ÖZDEN | Ayşe CAN

Yayıncı:

Istanbul University

Ana Başlıklar:

Farmakoloji ve terapötik

Dergi:

Journal of Faculty of Pharmacy of Istanbul University 2014 Vol.44 Issue 1, pp.1-10

Belge Tipi:

Makale

Yayın Tarihi:

1/2015

Belge Dili:

İngilizce

Document ID (Org No.):

2000513200

Sıklık:

Aylık

eISSN:

2587-2087