Determination of the Potential Use of Rhizosphere Bacteria in in vitro and in vivo Conditions for the Biological Control of Bacterial Seedling Blight Disease in Melon

Aysel Zübeyde Erdevil; Yesim Aysan

Makale detayları

Determination of the Potential Use of Rhizosphere Bacteria in in vitro and in vivo Conditions for the Biological Control of Bacterial Seedling Blight Disease in Melon

Yazarlar:

Aysel Zübeyde Erdevil | Yesim Aysan

Yayıncı:

Cukurova University

Ana Başlıklar:

Ziraat Mühendisliği (Diğer)

Dergi:

Çukurova Tarım ve Gıda Bilimleri Dergisi Vol.39 Issue 2, pp.352-366

Belge Tipi:

Makale

Yayın Tarihi:

12/2024

Belge Dili:

Türkçe

Document ID (Org No.):

2001337438

pISSN:

2636-7874

Anahtar kelimeler:

Özet:

 In this study, the potential use of rhizosphere bacterial isolates in the biological control of Bacterial Seedling Blight Disease in melon caused by Acidovorax citrulli was investigated under in vitro and in vivo conditions. A total of 398 candidate antagonist bacteria, including 241 soil samples from the rhizosphere of various plants and 157 isolates from our laboratory collection, were screened for their in vitro antagonistic and plant growth promoting mechanisms antibacterial activity, siderophore production capability, phosphorus solubilization ability, and indole acetic acid production levels. As a result of these tests, 8 bacterial isolates were selected for use in biological seed trials. In the first seed trial, the 8 applications suppressed disease severity by 0.48-39.45. In the second seed trial with the two most successful isolates, Y90 isolate prevented the disease by 46.37 while Y28 isolate prevented the disease by 18.97. Bacillus amyloliquefaciens subsp. plantarum Y90 and Providencia rettgeri Y28 were identified as successful antagonist bacterial isolates.

 In this study, the potential use of rhizosphere bacterial isolates in the biological control of Bacterial Seedling Blight Disease in melon caused by Acidovorax citrulli was investigated under in vitro and in vivo conditions. A total of 398 candidate antagonist bacteria, including 241 soil samples from the rhizosphere of various plants and 157 isolates from our laboratory collection, were screened for their in vitro antagonistic and plant growth promoting mechanisms antibacterial activity, siderophore production capability, phosphorus solubilization ability, and indole acetic acid production levels. As a result of these tests, 8 bacterial isolates were selected for use in biological seed trials. In the first seed trial, the 8 applications suppressed disease severity by 0.48-39.45. In the second seed trial with the two most successful isolates, Y90 isolate prevented the disease by 46.37 while Y28 isolate prevented the disease by 18.97. Bacillus amyloliquefaciens subsp. plantarum Y90 and Providencia rettgeri Y28 were identified as successful antagonist bacterial isolates.

 In this study, the potential use of rhizosphere bacterial isolates in the biological control of Bacterial Seedling Blight Disease in melon caused by Acidovorax citrulli was investigated under in vitro and in vivo conditions. A total of 398 candidate antagonist bacteria, including 241 soil samples from the rhizosphere of various plants and 157 isolates from our laboratory collection, were screened for their in vitro antagonistic and plant growth promoting mechanisms antibacterial activity, siderophore production capability, phosphorus solubilization ability, and indole acetic acid production levels. As a result of these tests, 8 bacterial isolates were selected for use in biological seed trials. In the first seed trial, the 8 applications suppressed disease severity by 0.48-39.45. In the second seed trial with the two most successful isolates, Y90 isolate prevented the disease by 46.37 while Y28 isolate prevented the disease by 18.97. Bacillus amyloliquefaciens subsp. plantarum Y90 and Providencia rettgeri Y28 were identified as successful antagonist bacterial isolates.

 Bu çalışmada, Acidovorax citrullinin neden olduğu kavunda Bakteriyel Fide Yanıklığı Hastalığının biyolojik mücadelesinde rizosfer bakterilerinin kullanım olanakları in vitro ve in vivo koşullarda araştırılmıştır. Bu amaçla çeşitli bitkilerin rizosferinden alınan toprak örneklerinden 241 adet, laboratuvarımız koleksiyonundan 157 adet olmak üzere toplam 398 aday antagonist bakterinin in vitro antagonistik ve bitki gelişimini teşvik eden etki mekanizmaları antibakteriyel etki, siderofor üretme yeteneği, fosforu çözme yeteneği ve indol asetik asit üretme miktarları incelenmiştir. Bu testler sonucunda, 8 bakteriyel izolatın biyokontrol potansiyelleri in vivo koşullarda tohum denemeleri ile belirlenmiştir. Birinci tohum denemesinde, 8 uygulama hastalık şiddetini 0.48-39.45 oranında baskılamıştır. En başarılı iki izolat ile kurulan ikinci tohum denemesinde ise Y90 izolatı hastalığı 46.37 oranında engellerken Y28 izolatı hastalığı 18.97 oranında baskılamıştır. Başarılı antagonist bakteriyel izolatlar Bacillus amyloliquefaciens subsp. plantarum Y90 ve Providencia rettgeri Y28 olarak tanılanmıştır.

Makale detayları

Determination of the Potential Use of Rhizosphere Bacteria in in vitro and in vivo Conditions for the Biological Control of Bacterial Seedling Blight Disease in Melon

Yazarlar:

Aysel Zübeyde Erdevil | Yesim Aysan

Yayıncı:

Cukurova University

Ana Başlıklar:

Ziraat Mühendisliği (Diğer)

Dergi:

Çukurova Tarım ve Gıda Bilimleri Dergisi Vol.39 Issue 2, pp.352-366

Belge Tipi:

Makale

Yayın Tarihi:

12/2024

Belge Dili:

Türkçe

Document ID (Org No.):

2001337438

pISSN:

2636-7874

Acidovorax citrulli

Melon

Antagonist

Biological ControlAcidovorax citrulli

Kavun

Antagonist

Biyolojik Mücadele

Sayı

Makale detayları

Yazarlar: Aysel Zübeyde Erdevil | Yesim Aysan

Yayıncı: Cukurova University

Ana Başlıklar: Ziraat Mühendisliği (Diğer)

Dergi: Çukurova Tarım ve Gıda Bilimleri Dergisi Vol.39 Issue 2, pp.352-366

Belge Tipi: Makale

Yayın Tarihi: 12/2024

Belge Dili: Türkçe

Document ID (Org No.): 2001337438

pISSN: 2636-7874

Sayı

Yazarlar:

Aysel Zübeyde Erdevil | Yesim Aysan

Yayıncı:

Cukurova University

Ana Başlıklar:

Ziraat Mühendisliği (Diğer)

Dergi:

Çukurova Tarım ve Gıda Bilimleri Dergisi Vol.39 Issue 2, pp.352-366

Belge Tipi:

Makale

Yayın Tarihi:

12/2024

Belge Dili:

Türkçe

Document ID (Org No.):

2001337438

pISSN:

2636-7874