Evaluation of the Effects of Acute Cisplatin Administration on Testicular and Ovarian Tissue in Rats

Betül Yalçın; Kübra Tuğçe Kalkan; Sedat Çarkıt; Özge Cengiz Mat; Gözde Özge Önder; Arzu Yay

Makale detayları

Evaluation of the Effects of Acute Cisplatin Administration on Testicular and Ovarian Tissue in Rats

Yazarlar:

Betül Yalçın | Kübra Tuğçe Kalkan | Sedat Çarkıt | Özge Cengiz Mat | Gözde Özge Önder | Arzu Yay

Yayıncı:

Duzce University

Ana Başlıklar:

Histology and Embryology

Dergi:

Duzce Medical Journal Vol.27 Issue 1, pp.88-94

Belge Tipi:

Makale

Yayın Tarihi:

4/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001338386

pISSN:

1307-671X

Anahtar kelimeler:

Özet:

 Aim: Cisplatin, one of the effective chemotherapeutics in cancer treatment, has the potential to affect the testis and ovary, leading to permanent or temporary infertility. This study aimed to determine the acute effects of cisplatin on testis and ovary histology and to investigate whether it induces any changes in immunohistochemical cyclooxygenase-2 COX2, nuclear factor kappa B p65 NFκB-p65, and heat-shock protein 70 HSP70 levels. Material and Methods: The study was planned as four groups: male control, male cisplatin, female control, and female cisplatin. The cisplatin group rats were adminestered with 7 mgkg cisplatin, and all rats were sacrificed 24 hours later. Hematoxylin-eosin and Massons trichrome stains were applied to testis and ovary tissues to examine their histopathological structure, and an immunohistochemistry staining protocol was applied to determine immunostaining intensity of COX2, NFκB-p65, and HSP70. Results: In the cisplatin group, a decrease in seminiferous tubule epithelium, an elevation in fibrotic response in the interstitial area, and a notable reduction in Johnson testicular biopsy score p0.001 were seen in the testis. In the ovary, atretic follicles p0.006 and luteal structures within the cortex, as well as vascular congestion p0.001, edema p0.001, and fibrotic areas within the medulla, were evident. These alterations resulted in a statistically significant increase in ovarian histoscores, except for leukocyte infiltration p0.322. In both tissues, cisplatin significantly increased the immunostaining intensity of COX2, NFκB-p65, and HSP70 compared to the control group. Conclusion: Acute cisplatin administration can induce tissue damage and pro-inflammatory response in the testis and ovary.

 Aim: Cisplatin, one of the effective chemotherapeutics in cancer treatment, has the potential to affect the testis and ovary, leading to permanent or temporary infertility. This study aimed to determine the acute effects of cisplatin on testis and ovary histology and to investigate whether it induces any changes in immunohistochemical cyclooxygenase-2 COX2, nuclear factor kappa B p65 NFκB-p65, and heat-shock protein 70 HSP70 levels. Material and Methods: The study was planned as four groups: male control, male cisplatin, female control, and female cisplatin. The cisplatin group rats were adminestered with 7 mgkg cisplatin, and all rats were sacrificed 24 hours later. Hematoxylin-eosin and Massons trichrome stains were applied to testis and ovary tissues to examine their histopathological structure, and an immunohistochemistry staining protocol was applied to determine immunostaining intensity of COX2, NFκB-p65, and HSP70. Results: In the cisplatin group, a decrease in seminiferous tubule epithelium, an elevation in fibrotic response in the interstitial area, and a notable reduction in Johnson testicular biopsy score p0.001 were seen in the testis. In the ovary, atretic follicles p0.006 and luteal structures within the cortex, as well as vascular congestion p0.001, edema p0.001, and fibrotic areas within the medulla, were evident. These alterations resulted in a statistically significant increase in ovarian histoscores, except for leukocyte infiltration p0.322. In both tissues, cisplatin significantly increased the immunostaining intensity of COX2, NFκB-p65, and HSP70 compared to the control group. Conclusion: Acute cisplatin administration can induce tissue damage and pro-inflammatory response in the testis and ovary.

 Aim: Cisplatin, one of the effective chemotherapeutics in cancer treatment, has the potential to affect the testis and ovary, leading to permanent or temporary infertility. This study aimed to determine the acute effects of cisplatin on testis and ovary histology and to investigate whether it induces any changes in immunohistochemical cyclooxygenase-2 COX2, nuclear factor kappa B p65 NFκB-p65, and heat-shock protein 70 HSP70 levels. Material and Methods: The study was planned as four groups: male control, male cisplatin, female control, and female cisplatin. The cisplatin group rats were adminestered with 7 mgkg cisplatin, and all rats were sacrificed 24 hours later. Hematoxylin-eosin and Massons trichrome stains were applied to testis and ovary tissues to examine their histopathological structure, and an immunohistochemistry staining protocol was applied to determine immunostaining intensity of COX2, NFκB-p65, and HSP70. Results: In the cisplatin group, a decrease in seminiferous tubule epithelium, an elevation in fibrotic response in the interstitial area, and a notable reduction in Johnson testicular biopsy score p0.001 were seen in the testis. In the ovary, atretic follicles p0.006 and luteal structures within the cortex, as well as vascular congestion p0.001, edema p0.001, and fibrotic areas within the medulla, were evident. These alterations resulted in a statistically significant increase in ovarian histoscores, except for leukocyte infiltration p0.322. In both tissues, cisplatin significantly increased the immunostaining intensity of COX2, NFκB-p65, and HSP70 compared to the control group. Conclusion: Acute cisplatin administration can induce tissue damage and pro-inflammatory response in the testis and ovary.

 Amaç: Kanser tedavisinde etkili kemoterapötiklerden biri olan sisplatin, testis ve overi etkileyerek kalıcı veya geçici infertiliteye yol açma potansiyeline sahiptir. Bu çalışma, sisplatinin testis ve over histolojisi üzerindeki akut etkilerini belirlemeyi ve immünohistokimyasal siklooksijenaz-2 COX2, nükleer faktör kappa B p65 NFκB-p65 ve ısı-şok protein 70 HSP70 düzeylerinde herhangi bir değişikliğe neden olup olmadığını araştırmayı amaçlamıştır. Gereç ve Yöntemler: Çalışma dört grup olarak planlandı: erkek kontrol, erkek sisplatin, dişi kontrol ve dişi sisplatin. Sisplatin grubu sıçanlara 7 mgkg sisplatin uygulandı ve tüm sıçanlar 24 saat sonra sakrifiye edildi. Testis ve over dokularına histopatolojik yapılarını incelemek amacıyla hematoksilen-eozin ve Masson trikrom boyaları uygulandı ve COX2, NFκB-p65 ve HSP70in immün boyama yoğunluğunu belirlemek amacıyla immünohistokimya boyama protokolü uygulandı. Bulgular: Sisplatin grubunda, testiste seminifer tübül epitelinde azalma, interstisyel alanda fibrotik yanıtta artış ve Johnson testiküler biyopsi skorunda kayda değer bir azalma p0,001 görüldü. Overde, kortekste atretik foliküller p0,006 ve luteal yapılar yanı sıra medullada vasküler konjesyon p0,001, ödem p0,001 ve fibrotik alanlar belirgindi. Bu değişiklikler, lökosit infiltrasyonu p0,322 dışında, over histoskorlamasında istatistiksel olarak anlamlı bir artışla sonuçlandı. Her iki dokuda da, sisplatin, kontrol grubuna kıyasla COX2, NFκB-p65 ve HSP70 immün boyanma yoğunluğunu önemli ölçüde artırdı. artışa neden oldu. Sonuç: Akut sisplatin uygulaması, testiste ve overde doku hasarına ve proinflamatuar yanıta yol açabilir.

Makale detayları

Evaluation of the Effects of Acute Cisplatin Administration on Testicular and Ovarian Tissue in Rats

Yazarlar:

Betül Yalçın | Kübra Tuğçe Kalkan | Sedat Çarkıt | Özge Cengiz Mat | Gözde Özge Önder | Arzu Yay

Yayıncı:

Duzce University

Ana Başlıklar:

Histology and Embryology

Dergi:

Duzce Medical Journal Vol.27 Issue 1, pp.88-94

Belge Tipi:

Makale

Yayın Tarihi:

4/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001338386

pISSN:

1307-671X

cisplatin

testis

ovary

cyclooxygenase-2

NF-kappa B p65

heat-shock protein 70sisplatin

testis

over

siklooksijenaz-2

NF-kappa B p65

ısı-şok protein 70

Sayı

Makale detayları

Yazarlar: Betül Yalçın | Kübra Tuğçe Kalkan | Sedat Çarkıt | Özge Cengiz Mat | Gözde Özge Önder | Arzu Yay

Yayıncı: Duzce University

Ana Başlıklar: Histology and Embryology

Dergi: Duzce Medical Journal Vol.27 Issue 1, pp.88-94

Belge Tipi: Makale

Yayın Tarihi: 4/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001338386

pISSN: 1307-671X

Sayı

Yazarlar:

Betül Yalçın | Kübra Tuğçe Kalkan | Sedat Çarkıt | Özge Cengiz Mat | Gözde Özge Önder | Arzu Yay

Yayıncı:

Duzce University

Ana Başlıklar:

Histology and Embryology

Dergi:

Duzce Medical Journal Vol.27 Issue 1, pp.88-94

Belge Tipi:

Makale

Yayın Tarihi:

4/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001338386

pISSN:

1307-671X