Staphylococcus aureus and Staphylococcal enterotoxin detection in raw milk and cheese origin coagulase positive isolates

Tuba YILDIRIM; Farid SADATİ; Berna KOCAMAN; Belgin SİRİKEN

doi:https://doi.org/10.38058/ijsl.596007

Makale detayları

Staphylococcus aureus and Staphylococcal enterotoxin detection in raw milk and cheese origin coagulase positive isolates

Yazarlar:

Tuba YILDIRIM | Farid SADATİ | Berna KOCAMAN | Belgin SİRİKEN

Yayıncı:

Amasya University

Ana Başlıklar:

Astronomi ve ilgili bilimler

Dergi:

International Journal of Science Letters 2019 Vol.1 Issue 1, pp.30-41

Belge Tipi:

Makale

Yayın Tarihi:

8/2019

Belge Dili:

İngilizce

Document ID (Org No.):

2000471227

Sıklık:

İki Yıllık

eISSN:

2687-4733

DOI:

https://doi.org/10.38058/ijsl.596007

Özet:

 


In
the present study, a total 110 (60 cheese and 50 cows’ raw milk) samples was analyzed
for CPS according to Food Drug Administration (FDA, 2001) previously. After the
isolation, to confirmation of the isolates, catalase test, microscopic
examination, coagulase test in tube and glucose-mannitol fermentation tests
were applied. After the tests, we obtained 97 CPS isolates. The 97 CPS isolates
were used as a material. For the confirmation of the isolates being Staphylococci species, 16S rRNA was
detected by using PCR assay. For the detection of the CPS isolates being S. aureus, nuc gene detected in the CPS isolates using PCR assay. To
determination of the staphylococcal enterotoxin, PCR assay was applied. The 16 S rRNA was detected in a total 97
(35 milk origin and 62 cheese origin) isolates. Therefore, these isolates were
evaluated as coagulase-positive
staphylococci (CPS).  The nuc gene was detected in 50 out of 97
CPS isolates. So, the 50 (18 cheese origin and 32 milk origin) isolates were
evaluated as S. aureus. However, none of the staphylococcal enterotoxin
genes 


(SE A,B,C,D,E,G,H,I,J)
was detected in 97 CPS or 50 S. aureus isolates.


.

Makale detayları

Staphylococcus aureus and Staphylococcal enterotoxin detection in raw milk and cheese origin coagulase positive isolates

Yazarlar: Tuba YILDIRIM | Farid SADATİ | Berna KOCAMAN | Belgin SİRİKEN

Yayıncı: Amasya University

Ana Başlıklar: Astronomi ve ilgili bilimler

Dergi: International Journal of Science Letters 2019 Vol.1 Issue 1, pp.30-41

Belge Tipi: Makale

Yayın Tarihi: 8/2019

Belge Dili: İngilizce

Document ID (Org No.): 2000471227

Sıklık: İki Yıllık

eISSN: 2687-4733

DOI: https://doi.org/10.38058/ijsl.596007

Sayı

Yazarlar:

Tuba YILDIRIM | Farid SADATİ | Berna KOCAMAN | Belgin SİRİKEN

Yayıncı:

Amasya University

Ana Başlıklar:

Astronomi ve ilgili bilimler

Dergi:

International Journal of Science Letters 2019 Vol.1 Issue 1, pp.30-41

Belge Tipi:

Makale

Yayın Tarihi:

8/2019

Belge Dili:

İngilizce

Document ID (Org No.):

2000471227

Sıklık:

İki Yıllık

eISSN:

2687-4733

DOI:

https://doi.org/10.38058/ijsl.596007