Caffeine Increases Apolipoprotein A-1 and Paraoxonase-1 but not Paraoxonase-3 Protein Levels in Human-Derived Liver (HepG2) Cells

Gülben Sayılan ÖZGÜN; Eray ÖZGÜN; Kıymet TABAKÇIOĞLU; Selma Süer GÖKMEN; Sevgi ESKİOCAK; Erol ÇAKIR

Makale detayları

Caffeine Increases Apolipoprotein A-1 and Paraoxonase-1 but not Paraoxonase-3 Protein Levels in Human-Derived Liver (HepG2) Cells

Yazarlar:

Gülben Sayılan ÖZGÜN | Eray ÖZGÜN | Kıymet TABAKÇIOĞLU | Selma Süer GÖKMEN | Sevgi ESKİOCAK | Erol ÇAKIR

Yayıncı:

Trakya University

Dergi:

Balkan Medical Journal 2017 Vol.34 Issue 6, pp.534-539

Belge Tipi:

Makale

Yayın Tarihi:

10/2017

Belge Dili:

Türkçe

Document ID (Org No.):

2000758976

Sıklık:

İki Aylık

pISSN:

2146-3123

Özet:

 

Background: Apolipoprotein A-1, paraoxonase-1 and paraoxonase-3 are antioxidant and anti-atherosclerotic structural high-density lipoprotein proteins that are mainly synthesized by the liver. No study has ever been performed to specifically examine the effects of caffeine on paraoxonase enzymes and on liver apolipoprotein A-1 protein levels. Aims: To investigate the dose-dependent effects of caffeine on liver apolipoprotein A-1, paraoxonase-1 and paraoxonase-3 protein levels. Study Design: In vitro experimental study.Methods: HepG2 cells were incubated with 0 (control), 10, 50 and 200 μM of caffeine for 24 hours. Cell viability was evaluated by 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assay. Apolipoprotein A-1, paraoxonase-1 and paraoxonase-3 protein levels were measured by western blotting.Results: We observed a significant increase on apolipoprotein A-1 and paraoxonase-1 protein levels in the cells incubated with 50 µM of caffeine and a significant increase on paraoxonase-1 protein level in the cells incubated with 200 µM of caffeine.Conclusion: Our study showed that caffeine does not change paraoxonase-3 protein level, but the higher doses used in our study do cause an increase in both apolipoprotein A-1 and paraoxonase-1 protein levels in liver cells.

Makale detayları

Caffeine Increases Apolipoprotein A-1 and Paraoxonase-1 but not Paraoxonase-3 Protein Levels in Human-Derived Liver (HepG2) Cells

Yazarlar: Gülben Sayılan ÖZGÜN | Eray ÖZGÜN | Kıymet TABAKÇIOĞLU | Selma Süer GÖKMEN | Sevgi ESKİOCAK | Erol ÇAKIR

Yayıncı: Trakya University

Dergi: Balkan Medical Journal 2017 Vol.34 Issue 6, pp.534-539

Belge Tipi: Makale

Yayın Tarihi: 10/2017

Belge Dili: Türkçe

Document ID (Org No.): 2000758976

Sıklık: İki Aylık

pISSN: 2146-3123

Sayı

Yazarlar:

Gülben Sayılan ÖZGÜN | Eray ÖZGÜN | Kıymet TABAKÇIOĞLU | Selma Süer GÖKMEN | Sevgi ESKİOCAK | Erol ÇAKIR

Yayıncı:

Trakya University

Dergi:

Balkan Medical Journal 2017 Vol.34 Issue 6, pp.534-539

Belge Tipi:

Makale

Yayın Tarihi:

10/2017

Belge Dili:

Türkçe

Document ID (Org No.):

2000758976

Sıklık:

İki Aylık

pISSN:

2146-3123