Temporal and Spatial Determination of Germin  Biosynthesis in Wheat Tissues

Mahmut ÇALIŞKAN; Andrew C. CUMING

Makale detayları

Temporal and Spatial Determination of Germin Biosynthesis in Wheat Tissues

Yazarlar:

Mahmut ÇALIŞKAN | Andrew C. CUMING

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2000 Vol.24 Issue 4, pp.775-782

Belge Tipi:

Makale

Yayın Tarihi:

3/2000

Belge Dili:

Türkçe

Document ID (Org No.):

2000813940

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Anahtar kelimeler:

Özet:

 

In the current study, the biosynthesis of germin was determined in the organs of wheat  embryos during germination. The temporal and spatial determination of germin protein was carried  out by employing an anti-germin sera in gel-blot analysis. Germin was detected in the coleoptile, roots  and vascular transition region of and 6-day-old germinated wheat seedlings. It was shown that the leaf  tissue acquires germin accumulation only at the late stage of germination. The pellet fraction of protein  homogenate obtained from embryos at different ages revealed higher germin accumulation than the  supernatant fraction from the same sources.

 

In the current study, the biosynthesis of germin was determined in the organs of wheat  embryos during germination. The temporal and spatial determination of germin protein was carried  out by employing an anti-germin sera in gel-blot analysis. Germin was detected in the coleoptile, roots  and vascular transition region of and 6-day-old germinated wheat seedlings. It was shown that the leaf  tissue acquires germin accumulation only at the late stage of germination. The pellet fraction of protein  homogenate obtained from embryos at different ages revealed higher germin accumulation than the  supernatant fraction from the same sources.

Makale detayları

Temporal and Spatial Determination of Germin Biosynthesis in Wheat Tissues

Yazarlar:

Mahmut ÇALIŞKAN | Andrew C. CUMING

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2000 Vol.24 Issue 4, pp.775-782

Belge Tipi:

Makale

Yayın Tarihi:

3/2000

Belge Dili:

Türkçe

Document ID (Org No.):

2000813940

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Early wheat embryo development

Germin

Western blotting.

Sayı

Makale detayları

Temporal and Spatial Determination of Germin Biosynthesis in Wheat Tissues

Yazarlar: Mahmut ÇALIŞKAN | Andrew C. CUMING

Yayıncı: TUBITAK

Dergi: Turkish Journal of Biology 2000 Vol.24 Issue 4, pp.775-782

Belge Tipi: Makale

Yayın Tarihi: 3/2000

Belge Dili: Türkçe

Document ID (Org No.): 2000813940

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

Early wheat embryo development

Germin

Western blotting.

Sayı

Yazarlar:

Mahmut ÇALIŞKAN | Andrew C. CUMING

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2000 Vol.24 Issue 4, pp.775-782

Belge Tipi:

Makale

Yayın Tarihi:

3/2000

Belge Dili:

Türkçe

Document ID (Org No.):

2000813940

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152