DETERMINATION OF GROWTH CHARACTERISTICS OF THE FOOT AND MOUTH VIRUSES (A, O, ASIA-1) IN BHK-21 AN30 AND BHK-21 AN73 CELL CULTURES

Neslihan TAŞÇENE; Banu Bayri ÖZBİLGE; Sadık Onur KARAÇAM; Veli GÜLYAZ; Mustafa HASÖKSÜZ

Makale detayları

DETERMINATION OF GROWTH CHARACTERISTICS OF THE FOOT AND MOUTH VIRUSES (A, O, ASIA-1) IN BHK-21 AN30 AND BHK-21 AN73 CELL CULTURES

Yazarlar:

Neslihan TAŞÇENE | Banu Bayri ÖZBİLGE | Sadık Onur KARAÇAM | Veli GÜLYAZ | Mustafa HASÖKSÜZ

Yayıncı:

İlker ÇAMKERTEN

Ana Başlıklar:

Hayvancılık

Dergi:

Journal of Advances in VetBio Science and Techniques 2017 Vol.2 Issue 1, pp.1-5

Belge Tipi:

Makale

Yayın Tarihi:

4/2017

Belge Dili:

İngilizce

Document ID (Org No.):

2000489132

Sıklık:

Üç Yıllık

eISSN:

2548-1150

Özet:

 

In
this study, it was aimed to investigate BHK-21An73 and BHK-21An30
growth rate of cell cultures and the effects on foot and mouth disease (FMD)
vaccine virus strains titers. For this purpose, growth rate of suspended cell
cultures of BHK-21An73 and BHK-21An30 were determined. A
TUR 11, O TUR 07 and Asia-1/11 strains of foot and mouth disease (FMD) vaccine
virus strains were produced separately on BHK-21An30 and BHK-21An73.
BHK-21An30 cell amounts during seven incubation days of
reached to peak level in monolayer form at the 4th day with 1,3x106/ml
cell numbers, in suspension form at the 4th day with 4,1x105 cell
numbers. BHK-21An73 reached to peak level in monolayer form at the
6th day 2,2x106/ml cell numbers while in suspension form at the 3rd
day with 2,8 x105 cell numbers. After the production of A, O and
Asia-1 vaccine viruses, in suspension BHK-21An30 cell cultures
average of 146S values respectively; 0,51 μg/ml, 0,18 μg/ml and 0,16 μg/ml were
detected while infective titers were determined as average 6,87 (Plaque Forming
Units) pfu/ml, 6,22 pfu/ml and 6,49 pfu/ml. After the production of A, O and
Asia-1 vaccine viruses, in suspended BHK-21An73 cell cultures
average of 146S values respectively; 2,11 μg/ml, 2,59 μg/ml and 0,53 μg/ml were
detected while infective titers were determined as average 6,99 pfu/ml, 7,82
pfu/ml and 6,37 pfu/ml. As a result, in the production of A, O and Asia-1
vaccine viruses in BHK-21An73, resulted in higher 146S values and
higher infective titers than BHK-21An30. It is concluded that for
FMD vaccine production the using of BHK-21An73 cell culture is more
appropriate for both of cost of manufacture and productive time.

 





Sonuç olarak, aşı üretiminde kullanılan A, O ve Asia-1
aşı viruslarının BHK-21 An73 hücre kültüründe daha yüksek 146S ve
enfektif titrede üreme gösterdiği ve bundan dolayı şap aşısı üretiminde BHK-21
An73 hücre kültürlerinin kullanılmasının gerek üretim zamanı gerekse
maliyet olarak BHK-21 An30 hücre kültürlerinden daha uygun olduğu
kanaatine varılmıştır. 


.

Makale detayları

DETERMINATION OF GROWTH CHARACTERISTICS OF THE FOOT AND MOUTH VIRUSES (A, O, ASIA-1) IN BHK-21 AN30 AND BHK-21 AN73 CELL CULTURES

Yazarlar: Neslihan TAŞÇENE | Banu Bayri ÖZBİLGE | Sadık Onur KARAÇAM | Veli GÜLYAZ | Mustafa HASÖKSÜZ

Yayıncı: İlker ÇAMKERTEN

Ana Başlıklar: Hayvancılık

Dergi: Journal of Advances in VetBio Science and Techniques 2017 Vol.2 Issue 1, pp.1-5

Belge Tipi: Makale

Yayın Tarihi: 4/2017

Belge Dili: İngilizce

Document ID (Org No.): 2000489132

Sıklık: Üç Yıllık

eISSN: 2548-1150

Sayı

Yazarlar:

Neslihan TAŞÇENE | Banu Bayri ÖZBİLGE | Sadık Onur KARAÇAM | Veli GÜLYAZ | Mustafa HASÖKSÜZ

Yayıncı:

İlker ÇAMKERTEN

Ana Başlıklar:

Hayvancılık

Dergi:

Journal of Advances in VetBio Science and Techniques 2017 Vol.2 Issue 1, pp.1-5

Belge Tipi:

Makale

Yayın Tarihi:

4/2017

Belge Dili:

İngilizce

Document ID (Org No.):

2000489132

Sıklık:

Üç Yıllık

eISSN:

2548-1150