Effects of Glycation on Erythrocyte Carbonic  Anhydrase-I and II in Patients With Diabetes  Mellitus

Hasan ÖZDEMİR

Makale detayları

Effects of Glycation on Erythrocyte Carbonic Anhydrase-I and II in Patients With Diabetes Mellitus

Yazarlar:

Hasan ÖZDEMİR

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2000 Vol.30 Issue 2, pp.135-142

Belge Tipi:

Makale

Yayın Tarihi:

1/2000

Belge Dili:

Türkçe

Document ID (Org No.):

2000757679

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Anahtar kelimeler:

Özet:

 

In order to investigate the effect  of high blood glucose levels of long duration  on carbonic anhydrase, and to evaluate the  relation of the enzyme to its inhibitors in  uncontrolled Type 1 diabetic patients,  erythrocyte CA -I and CA-II activities and  their kinetic parameters were determined.  The effects of glycation on erythrocyte  carbonic anhydrase-I and II (CA-I and CA-II)  in patients with Type-I diabetes mellitus  (uncontrolled) were investigated using kinetic  parameters. After blood glucose and  hemolysate total esterase activity levels had  been measured in both 10 control and 10  diabetic subjects, CA-I and CA-II in  hemolysate were purified separately by  affinity chromatography. The enzyme  activity, fructosamines, V max , K M , and K i  values of CA-I and CA-II were determined.  The means of the blood glucose and  hemolysate total esterase activity levels were  significantly higher in the diabetics than in  the controls ( p  0.05). The means of the K i values of  both CA-I and CA-II, using acetazolamide and  sulfanilamide inhibitors, were significantly  lower in the diabetics than in the controls ( p<  0.001). Glycation was found to increase both  CA activity and the inhibitory effect of  acetazolamide and sulfonamide on CA  activity. Since CA is a well-known enzyme  regulating pH in most of the tissues in the  body, changes in CA activity may be  associated with metabolic diseases, especially  in diabetes mellitus. Therefore, dosages of  CA inhibitors should be considered carefully  in the treatment of diabetic patients.

 

In order to investigate the effect  of high blood glucose levels of long duration  on carbonic anhydrase, and to evaluate the  relation of the enzyme to its inhibitors in  uncontrolled Type 1 diabetic patients,  erythrocyte CA -I and CA-II activities and  their kinetic parameters were determined.  The effects of glycation on erythrocyte  carbonic anhydrase-I and II (CA-I and CA-II)  in patients with Type-I diabetes mellitus  (uncontrolled) were investigated using kinetic  parameters. After blood glucose and  hemolysate total esterase activity levels had  been measured in both 10 control and 10  diabetic subjects, CA-I and CA-II in  hemolysate were purified separately by  affinity chromatography. The enzyme  activity, fructosamines, V max , K M , and K i  values of CA-I and CA-II were determined.  The means of the blood glucose and  hemolysate total esterase activity levels were  significantly higher in the diabetics than in  the controls ( p  0.05). The means of the K i values of  both CA-I and CA-II, using acetazolamide and  sulfanilamide inhibitors, were significantly  lower in the diabetics than in the controls ( p<  0.001). Glycation was found to increase both  CA activity and the inhibitory effect of  acetazolamide and sulfonamide on CA  activity. Since CA is a well-known enzyme  regulating pH in most of the tissues in the  body, changes in CA activity may be  associated with metabolic diseases, especially  in diabetes mellitus. Therefore, dosages of  CA inhibitors should be considered carefully  in the treatment of diabetic patients.

Makale detayları

Effects of Glycation on Erythrocyte Carbonic Anhydrase-I and II in Patients With Diabetes Mellitus

Yazarlar:

Hasan ÖZDEMİR

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2000 Vol.30 Issue 2, pp.135-142

Belge Tipi:

Makale

Yayın Tarihi:

1/2000

Belge Dili:

Türkçe

Document ID (Org No.):

2000757679

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Carbonic anhydrase

glycation

enzyme kinetics.

Sayı

Makale detayları

Effects of Glycation on Erythrocyte Carbonic Anhydrase-I and II in Patients With Diabetes Mellitus

Yazarlar: Hasan ÖZDEMİR

Yayıncı: TUBITAK

Dergi: Turkish Journal of Medical Sciences 2000 Vol.30 Issue 2, pp.135-142

Belge Tipi: Makale

Yayın Tarihi: 1/2000

Belge Dili: Türkçe

Document ID (Org No.): 2000757679

Sıklık: İki Aylık

eISSN: 1303-6165

pISSN: 1300-0144

Carbonic anhydrase

glycation

enzyme kinetics.

Sayı

Yazarlar:

Hasan ÖZDEMİR

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2000 Vol.30 Issue 2, pp.135-142

Belge Tipi:

Makale

Yayın Tarihi:

1/2000

Belge Dili:

Türkçe

Document ID (Org No.):

2000757679

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144