In vitro study on chondrogenic differentiation of human adipose-derived stem cells on treated bovine pericardium

My Thi Ngoc NGUYEN; Vu Nguyen DOAN; Ha Le Bao TRAN

Makale detayları

In vitro study on chondrogenic differentiation of human adipose-derived stem cells on treated bovine pericardium

Yazarlar:

My Thi Ngoc NGUYEN | Vu Nguyen DOAN | Ha Le Bao TRAN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2019 Vol.43 Issue 6, pp.360-370

Belge Tipi:

Makale

Yayın Tarihi:

11/2019

Belge Dili:

İngilizce

Document ID (Org No.):

2000811046

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Özet:

 

Bovine pericardium has been proposed as an available material for tissue engineering and bioprosthetic reconstruction. In this  study, bovine pericardium was fabricated into a scaffold for culturing and chondrogenic differentiation of human adipose-derived stem  cells (hADSCs). Bovine pericardium was treated in 10 mM Tris-HCl and 0.15% SDS, followed by crosslinking in 0.1% glutaraldehyde.  Treated bovine pericardium (tBP) was characterized as a slight yellowish thin membrane with enhanced tensile strength and strain  property. The membrane maintained stability under enzymatic conditions for up to 16 days of incubation. The results confirmed tBP  as a cell-friendly scaffold for hADSCs due to low cytotoxicity and its ability to support an appropriate attachment and proliferation of  hADSCs. Moreover, there was an accumulation of the extracellular matrix proteoglycan in tBP seeded with hADSCs after 7 and 14  days of chondrogenic induction. COMP as a specific marker of chondrogenesis was detected after 7 days, whereas type X-a1 collagen  (Col10a1) expression was stable up to day 14. However, minor expression of aggrecan was found. Taken together, these results indicate  that tBP is a potential scaffold for hADSCs for cartilage tissue engineering.

Makale detayları

In vitro study on chondrogenic differentiation of human adipose-derived stem cells on treated bovine pericardium

Yazarlar: My Thi Ngoc NGUYEN | Vu Nguyen DOAN | Ha Le Bao TRAN

Yayıncı: TUBITAK

Dergi: Turkish Journal of Biology 2019 Vol.43 Issue 6, pp.360-370

Belge Tipi: Makale

Yayın Tarihi: 11/2019

Belge Dili: İngilizce

Document ID (Org No.): 2000811046

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

Sayı

Yazarlar:

My Thi Ngoc NGUYEN | Vu Nguyen DOAN | Ha Le Bao TRAN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2019 Vol.43 Issue 6, pp.360-370

Belge Tipi:

Makale

Yayın Tarihi:

11/2019

Belge Dili:

İngilizce

Document ID (Org No.):

2000811046

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152