The Effects of Biotics Derived from Lactiplantibacillus plantarum EIR/IF-1 on the Modulation of Periodontal Inflammation

Hikmet Can; Hazal Kibar Demirhan; Emine Omer Oglou; Fadime Kıran

Makale detayları

The Effects of Biotics Derived from Lactiplantibacillus plantarum EIR/IF-1 on the Modulation of Periodontal Inflammation

Yazarlar:

Hikmet Can | Hazal Kibar Demirhan | Emine Omer Oglou | Fadime Kıran

Yayıncı:

Istanbul Aydin University

Ana Başlıklar:

Periodontoloji

Dergi:

Aydın Dental Journal Vol.11 Issue 1, pp.1-15

Belge Tipi:

Makale

Yayın Tarihi:

4/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001347238

pISSN:

2149-5572

Anahtar kelimeler:

Özet:

 Objective: This study aims to investigate the immunomodulatory effects of postbiotics and paraprobiotics derived from Lactiplantibacillus plantarum EIRIF-1, isolated from infant fecal microbiota. Materials and Methods: Human periodontal ligament fibroblast hPDLF cells were co-incubated with the release and bound forms of exopolysaccharides 10-1,000 μgmL, cell lysate 0.1-1,000 μgmL, cell surface proteins 0.1-100 μgmL, and inactivated cells 10⁶-10¹⁰ CFUmL derived from Lactiplantibacillus plantarum EIRIF-1 for 24 hours. Cell viability was assessed using the MTT assay. Lipopolysaccharide LPS from Porphyromonas gingivalis, a key pathogen involved in periodontal disease, was used to induce inflammation in hPDLF cells. Non-toxic doses of the biotics were subsequently tested for their impact on cytokine production in LPS induced hPDLF cells. Cytokine levels IL-10, IL-8, and IFN-γ were quantified using the ELISA protocol. Results: After 24 hours incubation of hPDLF cells with 1 µgmL LPS and selected concentrations of the biotics derived from the EIRIF-1 strain that did not exhibit toxic effects, it was observed that LPS from P. gingivalis induced IL-8 production. However, the biotics extracted from the EIRIF-1 strain significantly reduced IL-8 production p0.0001. Furthermore, these biotics increased the production of the anti-inflammatory cytokine IL-10 p0.0001. Conclusion: The obtained data suggest that the biotics have the potential to counteract the pro-inflammatory effects induced by LPS. The findings highlight the potential of postbiotics and paraprobiotics, containing biologically active and effective components, as a promising natural and reliable approach for reducing and preventing periodontal inflammation. In conclusion, our results suggest that microbiota-derived biotics could serve as effective adjuncts in the management of periodontal diseases.

 Objective: This study aims to investigate the immunomodulatory effects of postbiotics and paraprobiotics derived from Lactiplantibacillus plantarum EIRIF-1, isolated from infant fecal microbiota. Materials and Methods: Human periodontal ligament fibroblast hPDLF cells were co-incubated with the release and bound forms of exopolysaccharides 10-1,000 μgmL, cell lysate 0.1-1,000 μgmL, cell surface proteins 0.1-100 μgmL, and inactivated cells 10⁶-10¹⁰ CFUmL derived from Lactiplantibacillus plantarum EIRIF-1 for 24 hours. Cell viability was assessed using the MTT assay. Lipopolysaccharide LPS from Porphyromonas gingivalis, a key pathogen involved in periodontal disease, was used to induce inflammation in hPDLF cells. Non-toxic doses of the biotics were subsequently tested for their impact on cytokine production in LPS induced hPDLF cells. Cytokine levels IL-10, IL-8, and IFN-γ were quantified using the ELISA protocol. Results: After 24 hours incubation of hPDLF cells with 1 µgmL LPS and selected concentrations of the biotics derived from the EIRIF-1 strain that did not exhibit toxic effects, it was observed that LPS from P. gingivalis induced IL-8 production. However, the biotics extracted from the EIRIF-1 strain significantly reduced IL-8 production p0.0001. Furthermore, these biotics increased the production of the anti-inflammatory cytokine IL-10 p0.0001. Conclusion: The obtained data suggest that the biotics have the potential to counteract the pro-inflammatory effects induced by LPS. The findings highlight the potential of postbiotics and paraprobiotics, containing biologically active and effective components, as a promising natural and reliable approach for reducing and preventing periodontal inflammation. In conclusion, our results suggest that microbiota-derived biotics could serve as effective adjuncts in the management of periodontal diseases.

 Objective: This study aims to investigate the immunomodulatory effects of postbiotics and paraprobiotics derived from Lactiplantibacillus plantarum EIRIF-1, isolated from infant fecal microbiota. Materials and Methods: Human periodontal ligament fibroblast hPDLF cells were co-incubated with the release and bound forms of exopolysaccharides 10-1,000 μgmL, cell lysate 0.1-1,000 μgmL, cell surface proteins 0.1-100 μgmL, and inactivated cells 10⁶-10¹⁰ CFUmL derived from Lactiplantibacillus plantarum EIRIF-1 for 24 hours. Cell viability was assessed using the MTT assay. Lipopolysaccharide LPS from Porphyromonas gingivalis, a key pathogen involved in periodontal disease, was used to induce inflammation in hPDLF cells. Non-toxic doses of the biotics were subsequently tested for their impact on cytokine production in LPS induced hPDLF cells. Cytokine levels IL-10, IL-8, and IFN-γ were quantified using the ELISA protocol. Results: After 24 hours incubation of hPDLF cells with 1 µgmL LPS and selected concentrations of the biotics derived from the EIRIF-1 strain that did not exhibit toxic effects, it was observed that LPS from P. gingivalis induced IL-8 production. However, the biotics extracted from the EIRIF-1 strain significantly reduced IL-8 production p0.0001. Furthermore, these biotics increased the production of the anti-inflammatory cytokine IL-10 p0.0001. Conclusion: The obtained data suggest that the biotics have the potential to counteract the pro-inflammatory effects induced by LPS. The findings highlight the potential of postbiotics and paraprobiotics, containing biologically active and effective components, as a promising natural and reliable approach for reducing and preventing periodontal inflammation. In conclusion, our results suggest that microbiota-derived biotics could serve as effective adjuncts in the management of periodontal diseases.

 Amaç: Bu çalışma, bebek fekal mikrobiyotasından izole edilen Lactiplantibacillus plantarum EIRIF-1 suşundan elde edilen postbiyotiklerin ve paraprobiyotiklerin immünmodülatör etkilerini araştırmayı amaçlamaktadır. Materyal ve Yöntem: İnsan periodontal ligament fibroblast iPDLF hücreleri, Lactiplantibacillus plantarum EIRIF-1 kaynaklı salınan ve bağlı formlarda bulunan eksopolizakkaritler 101.000 μgmL, hücre lizatı 0,11.000 μgmL, hücre yüzeyi proteinleri 0,1100 μgmL ve inaktif hücreler 10⁶10¹⁰ KOBmL ile 24 saat süreyle birlikte inkübe edilmiştir. Hücre canlılığı MTT testi ile değerlendirilmiştir. Periodontal hastalıkla ilişkili önemli bir patojen olan Porphyromonas gingivalisten elde edilen lipopolisakkarit LPS, iPDLF hücrelerinde inflamasyonu indüklemek için kullanılmıştır. Biyotiklerin, LPS ile uyarılmış iPDLF hücrelerinde sitokin üretimine olan etkisi, toksik olmayan dozlarla birlikte inkübasyon neticesinde test edilmiştir. Sitokin seviyeleri IL-10, IL-8 ve IFN-γ ELİZA protokolü ile belirlenmiştir. Bulgular: Hücreler 1 µgmL LPS ve toksik etkisi olmayan seçilen biyotik dozları ile 24 saat inkübe edildikten sonra, P. gingivalis kaynaklı LPSnin IL-8 üretimini indüklediği gözlemlenmiştir. Ancak, EIRIF-1 suşundan elde edilen biyotikler, IL-8 üretimini önemli ölçüde azaltmıştır p0.0001. Ayrıca, bu biyotikler anti-inflamatuvar bir sitokin olan IL-10 üretimini artırmıştır p0.0001. Sonuç: Elde edilen veriler, biyotiklerin LPSnin oluşturduğu pro-inflamatuvar etkileri azaltabilecek potansiyele sahip olduğunu göstermektedir. Bu bulgular, biyolojik olarak aktif ve etkin bileşenler içeren postbiyotikler ve paraprobiyotiklerin periodontal inflamasyonu azaltmak ve önlemek için umut verici, doğal ve güvenilir bir yaklaşım olarak kullanılabileceğini vurgulamaktadır. Sonuç olarak, mikrobiyota kaynaklı biyotiklerin periodontal hastalıkların yönetiminde etkili yardımcı bir tedavi olarak kullanılabileceği önerilmektedir.

Makale detayları

The Effects of Biotics Derived from Lactiplantibacillus plantarum EIR/IF-1 on the Modulation of Periodontal Inflammation

Yazarlar:

Hikmet Can | Hazal Kibar Demirhan | Emine Omer Oglou | Fadime Kıran

Yayıncı:

Istanbul Aydin University

Ana Başlıklar:

Periodontoloji

Dergi:

Aydın Dental Journal Vol.11 Issue 1, pp.1-15

Belge Tipi:

Makale

Yayın Tarihi:

4/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001347238

pISSN:

2149-5572

Inflammation

Lactiplantibacillus plantarum

Paraprobiotics

Postbioticsİnflamasyon

Lactiplantibacillus plantarum

Paraprobiyotikler

Postbiyotikler

Sayı

Makale detayları

Yazarlar: Hikmet Can | Hazal Kibar Demirhan | Emine Omer Oglou | Fadime Kıran

Yayıncı: Istanbul Aydin University

Ana Başlıklar: Periodontoloji

Dergi: Aydın Dental Journal Vol.11 Issue 1, pp.1-15

Belge Tipi: Makale

Yayın Tarihi: 4/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001347238

pISSN: 2149-5572

Sayı

Yazarlar:

Hikmet Can | Hazal Kibar Demirhan | Emine Omer Oglou | Fadime Kıran

Yayıncı:

Istanbul Aydin University

Ana Başlıklar:

Periodontoloji

Dergi:

Aydın Dental Journal Vol.11 Issue 1, pp.1-15

Belge Tipi:

Makale

Yayın Tarihi:

4/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001347238

pISSN:

2149-5572