Poly-3-hydroxybutyrate-producing extreme halophilic archaeon: Haloferax sp. MA10 isolated from Çamaltı Saltern, İzmir

Ebru TEKİN; Mustafa ATEŞ; Özge KAHRAMAN

Makale detayları

Poly-3-hydroxybutyrate-producing extreme halophilic archaeon: Haloferax sp. MA10 isolated from Çamaltı Saltern, İzmir

Yazarlar:

Ebru TEKİN | Mustafa ATEŞ | Özge KAHRAMAN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2012 Vol.36 Issue 3, pp.303-312

Belge Tipi:

Makale

Yayın Tarihi:

2/2012

Belge Dili:

Türkçe

Document ID (Org No.):

2000812661

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Anahtar kelimeler:

Özet:

 

İzmir Çamaltı Saltern is the biggest seawater-based saltern in Turkey. To date, it has not been investigated extensively for the existence of poly-3-hydroxybutyrate (PHB)-producing species. In this study, 14 extremely halophilic archaea were isolated, purified, and screened for PHB production. One strain was then selected as the best PHB producer, further cultivated in different PHB media, and compared with a positive control, Haloferax mediterranei ATCC 33500. PHB was extracted from cells and measured with a spectrophotometer, and then the amount of PHB was measured through comparison with standard PHB. The detected high PHB yield was 6.53% of the dry cell weight in the PHB medium supplemented with acetate. Partial 16S rRNA gene sequence analysis showed 99% similarity to Haloferax alexandrinus strain TMT; therefore, the strain was named Haloferax sp. MA10. Haloferax sp. MA10 and Haloferax alexandrinus TMT have some differences in phenotypic and biochemical properties. With this study, the discovery of PHB-producing extreme halophilic archaeon Haloferax sp. MA10 at İzmir's Çamaltı Saltern is reported for the first time.

 

İzmir Çamaltı Saltern is the biggest seawater-based saltern in Turkey. To date, it has not been investigated extensively for the existence of poly-3-hydroxybutyrate (PHB)-producing species. In this study, 14 extremely halophilic archaea were isolated, purified, and screened for PHB production. One strain was then selected as the best PHB producer, further cultivated in different PHB media, and compared with a positive control, Haloferax mediterranei ATCC 33500. PHB was extracted from cells and measured with a spectrophotometer, and then the amount of PHB was measured through comparison with standard PHB. The detected high PHB yield was 6.53% of the dry cell weight in the PHB medium supplemented with acetate. Partial 16S rRNA gene sequence analysis showed 99% similarity to Haloferax alexandrinus strain TMT; therefore, the strain was named Haloferax sp. MA10. Haloferax sp. MA10 and Haloferax alexandrinus TMT have some differences in phenotypic and biochemical properties. With this study, the discovery of PHB-producing extreme halophilic archaeon Haloferax sp. MA10 at İzmir's Çamaltı Saltern is reported for the first time.

Makale detayları

Poly-3-hydroxybutyrate-producing extreme halophilic archaeon: Haloferax sp. MA10 isolated from Çamaltı Saltern, İzmir

Yazarlar:

Ebru TEKİN | Mustafa ATEŞ | Özge KAHRAMAN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2012 Vol.36 Issue 3, pp.303-312

Belge Tipi:

Makale

Yayın Tarihi:

2/2012

Belge Dili:

Türkçe

Document ID (Org No.):

2000812661

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Key words: Extremely halophilic archaeon

poly-3-hydroxybutyrate (PHB)

PHB extraction and quantification

Haloferax sp. MA10

16S rRNA gene

Sayı

Makale detayları

Poly-3-hydroxybutyrate-producing extreme halophilic archaeon: Haloferax sp. MA10 isolated from Çamaltı Saltern, İzmir

Yazarlar: Ebru TEKİN | Mustafa ATEŞ | Özge KAHRAMAN

Yayıncı: TUBITAK

Dergi: Turkish Journal of Biology 2012 Vol.36 Issue 3, pp.303-312

Belge Tipi: Makale

Yayın Tarihi: 2/2012

Belge Dili: Türkçe

Document ID (Org No.): 2000812661

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

Key words: Extremely halophilic archaeon

poly-3-hydroxybutyrate (PHB)

PHB extraction and quantification

Haloferax sp. MA10

16S rRNA gene

Sayı

Yazarlar:

Ebru TEKİN | Mustafa ATEŞ | Özge KAHRAMAN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2012 Vol.36 Issue 3, pp.303-312

Belge Tipi:

Makale

Yayın Tarihi:

2/2012

Belge Dili:

Türkçe

Document ID (Org No.):

2000812661

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152