Temperature-induced stress response in Lymantria dispar neurosecretory neurons

Larisa ILIJIN; Milena VLAHOVIC; Vesna Peric MATARUGA; Ivana KMETIČ; Anja GAVRILOVIĆ; Dragana MATIĆ; Marija MRDAKOVIĆ

Makale detayları

Temperature-induced stress response in Lymantria dispar neurosecretory neurons

Yazarlar:

Larisa ILIJIN | Milena VLAHOVIC | Vesna Peric MATARUGA | Ivana KMETIČ | Anja GAVRILOVIĆ | Dragana MATIĆ | Marija MRDAKOVIĆ

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2014 Vol.38 Issue 2, pp.157-167

Belge Tipi:

Makale

Yayın Tarihi:

5/2014

Belge Dili:

Türkçe

Document ID (Org No.):

2000812224

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Anahtar kelimeler:

Özet:

 

The release of neurosecretory material from A2 neurosecretory neurons (NSNs) was stimulated in Lymantria dispar fourth instar caterpillars exposed to a temperature of 35 °C for 1, 12, and 24 h, as well as those allowed recover after exposure (12 h at 35 °C, then 12 h at 23 °C). The levels of 2 protein forms with the same molecular mass as bombyxin (3-4 and 4-5 kDa) increased with prolonged exposure to 35 °C. The second band was present only in the groups exposed to this stressor. There was intensified synthetic activity and a low level of secretion in L2' NSNs after exposure to 35 °C. We previously found these NSNs to be immunopositive for prothoracicotropic neurohormone. After this stress, densitometric analysis revealed a decreased amount of the 11-12 kDa isoform (present in the control group). The new isoform (13-15 kDa), expressed after exposure of the insects to a high temperature, increased in amount with prolonged exposure and after recovery at 23 °C. Short-term exposure of caterpillars to high temperatures (35 °C) is a stressor and activates carbohydrate metabolism, while PTTH immunopositive NSNs are secretory-inactive during acute thermal stress regimes.

 

The release of neurosecretory material from A2 neurosecretory neurons (NSNs) was stimulated in Lymantria dispar fourth instar caterpillars exposed to a temperature of 35 °C for 1, 12, and 24 h, as well as those allowed recover after exposure (12 h at 35 °C, then 12 h at 23 °C). The levels of 2 protein forms with the same molecular mass as bombyxin (3-4 and 4-5 kDa) increased with prolonged exposure to 35 °C. The second band was present only in the groups exposed to this stressor. There was intensified synthetic activity and a low level of secretion in L2' NSNs after exposure to 35 °C. We previously found these NSNs to be immunopositive for prothoracicotropic neurohormone. After this stress, densitometric analysis revealed a decreased amount of the 11-12 kDa isoform (present in the control group). The new isoform (13-15 kDa), expressed after exposure of the insects to a high temperature, increased in amount with prolonged exposure and after recovery at 23 °C. Short-term exposure of caterpillars to high temperatures (35 °C) is a stressor and activates carbohydrate metabolism, while PTTH immunopositive NSNs are secretory-inactive during acute thermal stress regimes.

Makale detayları

Temperature-induced stress response in Lymantria dispar neurosecretory neurons

Yazarlar:

Larisa ILIJIN | Milena VLAHOVIC | Vesna Peric MATARUGA | Ivana KMETIČ | Anja GAVRILOVIĆ | Dragana MATIĆ | Marija MRDAKOVIĆ

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2014 Vol.38 Issue 2, pp.157-167

Belge Tipi:

Makale

Yayın Tarihi:

5/2014

Belge Dili:

Türkçe

Document ID (Org No.):

2000812224

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Key words: High temperature

gypsy moth

medial and lateral neurosecretory neurons

Sayı

Makale detayları

Temperature-induced stress response in Lymantria dispar neurosecretory neurons

Yazarlar: Larisa ILIJIN | Milena VLAHOVIC | Vesna Peric MATARUGA | Ivana KMETIČ | Anja GAVRILOVIĆ | Dragana MATIĆ | Marija MRDAKOVIĆ

Yayıncı: TUBITAK

Dergi: Turkish Journal of Biology 2014 Vol.38 Issue 2, pp.157-167

Belge Tipi: Makale

Yayın Tarihi: 5/2014

Belge Dili: Türkçe

Document ID (Org No.): 2000812224

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

Key words: High temperature

gypsy moth

medial and lateral neurosecretory neurons

Sayı

Yazarlar:

Larisa ILIJIN | Milena VLAHOVIC | Vesna Peric MATARUGA | Ivana KMETIČ | Anja GAVRILOVIĆ | Dragana MATIĆ | Marija MRDAKOVIĆ

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2014 Vol.38 Issue 2, pp.157-167

Belge Tipi:

Makale

Yayın Tarihi:

5/2014

Belge Dili:

Türkçe

Document ID (Org No.):

2000812224

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152