Chemical composition and antibacterial activity of Azadirachta indica seed oil from Chad

Brahim Mahamat Ousman; Omar Belmehdi

Makale detayları

Chemical composition and antibacterial activity of Azadirachta indica seed oil from Chad

Yazarlar:

Brahim Mahamat Ousman | Omar Belmehdi

Yayıncı:

İzzet KARA

Ana Başlıklar:

Microbiology (Other)

Dergi:

International Journal of Secondary Metabolite Vol.12 Issue 2, pp.429-447

Belge Tipi:

Makale

Yayın Tarihi:

1/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001340464

pISSN:

2148-6905

Anahtar kelimeler:

Özet:

 This study focuses on extracting oil from the seeds of the Azadirachta indica species A. Juss, known in Chad as mim or neem, utilizing a cold-press extraction method. It aims to characterize the oils chemical composition through Gas Chromatography-Mass Spectrometry GC-MS analysis and evaluate its antibacterial efficacy using the well diffusion and microdilution techniques. The antibacterial potential was assessed against four food-borne pathogens: Staphylococcus aureus, Methicillin-resistant Staphylococcus aureus MRSA, Escherichia coli, Salmonella sp., and a soil bacterium, Bacillus subtilis. The results from the GC-MS analysis indicated a predominant presence of fatty alcohols, notably Stigmasta-3,5-diene 49.00, a steroid alcohol, and Tetradecen-11-yn-1-ol 35.37, a long-chain fatty alcohol. Additionally, lesser quantities of compounds such as E, E-7,11,15-Trimethyl-3-methylene-hexadeca-1,6,10,14-tetraene a diterpene at 9.68, Squalene a triterpene at 1.77, and 2,4,4-Trimethyl-3-hydroxymethyl-5a-3-methyl-but-2-enyl-cyclohexene a sesquiterpene at 4.19 were identified. The analysis showed that neem oil is rich in fatty alcohols and phytosterols, with lower terpenes and phenolic compounds. It showed no significant antibacterial activity against the tested bacteria. This suggests that from cold-press extraction, neem oil may not effectively combat food-borne pathogens and soil bacteria due to its fatty alcohol and phytosterol content, along with the bacterias resistance. Increasing the concentration of crude oil in the antibacterial test could lead to positive results. The findings indicate a need for further research to isolate stronger antibacterial molecules in neem oil by separating its components, focusing on extraction methods and solvent polarity.

 This study focuses on extracting oil from the seeds of the Azadirachta indica species A. Juss, known in Chad as mim or neem, utilizing a cold-press extraction method. It aims to characterize the oils chemical composition through Gas Chromatography-Mass Spectrometry GC-MS analysis and evaluate its antibacterial efficacy using the well diffusion and microdilution techniques. The antibacterial potential was assessed against four food-borne pathogens: Staphylococcus aureus, Methicillin-resistant Staphylococcus aureus MRSA, Escherichia coli, Salmonella sp., and a soil bacterium, Bacillus subtilis. The results from the GC-MS analysis indicated a predominant presence of fatty alcohols, notably Stigmasta-3,5-diene 49.00, a steroid alcohol, and Tetradecen-11-yn-1-ol 35.37, a long-chain fatty alcohol. Additionally, lesser quantities of compounds such as E, E-7,11,15-Trimethyl-3-methylene-hexadeca-1,6,10,14-tetraene a diterpene at 9.68, Squalene a triterpene at 1.77, and 2,4,4-Trimethyl-3-hydroxymethyl-5a-3-methyl-but-2-enyl-cyclohexene a sesquiterpene at 4.19 were identified. The analysis showed that neem oil is rich in fatty alcohols and phytosterols, with lower terpenes and phenolic compounds. It showed no significant antibacterial activity against the tested bacteria. This suggests that from cold-press extraction, neem oil may not effectively combat food-borne pathogens and soil bacteria due to its fatty alcohol and phytosterol content, along with the bacterias resistance. Increasing the concentration of crude oil in the antibacterial test could lead to positive results. The findings indicate a need for further research to isolate stronger antibacterial molecules in neem oil by separating its components, focusing on extraction methods and solvent polarity.

 This study focuses on extracting oil from the seeds of the Azadirachta indica species A. Juss, known in Chad as mim or neem, utilizing a cold-press extraction method. It aims to characterize the oils chemical composition through Gas Chromatography-Mass Spectrometry GC-MS analysis and evaluate its antibacterial efficacy using the well diffusion and microdilution techniques. The antibacterial potential was assessed against four food-borne pathogens: Staphylococcus aureus, Methicillin-resistant Staphylococcus aureus MRSA, Escherichia coli, Salmonella sp., and a soil bacterium, Bacillus subtilis. The results from the GC-MS analysis indicated a predominant presence of fatty alcohols, notably Stigmasta-3,5-diene 49.00, a steroid alcohol, and Tetradecen-11-yn-1-ol 35.37, a long-chain fatty alcohol. Additionally, lesser quantities of compounds such as E, E-7,11,15-Trimethyl-3-methylene-hexadeca-1,6,10,14-tetraene a diterpene at 9.68, Squalene a triterpene at 1.77, and 2,4,4-Trimethyl-3-hydroxymethyl-5a-3-methyl-but-2-enyl-cyclohexene a sesquiterpene at 4.19 were identified. The analysis showed that neem oil is rich in fatty alcohols and phytosterols, with lower terpenes and phenolic compounds. It showed no significant antibacterial activity against the tested bacteria. This suggests that from cold-press extraction, neem oil may not effectively combat food-borne pathogens and soil bacteria due to its fatty alcohol and phytosterol content, along with the bacterias resistance. Increasing the concentration of crude oil in the antibacterial test could lead to positive results. The findings indicate a need for further research to isolate stronger antibacterial molecules in neem oil by separating its components, focusing on extraction methods and solvent polarity.

 This study focuses on extracting oil from the seeds of the Azadirachta indica species A. Juss, known in Chad as mim or neem, utilizing a cold-press extraction method. It aims to characterize the oils chemical composition through Gas Chromatography-Mass Spectrometry GC-MS analysis and evaluate its antibacterial efficacy using the well diffusion and microdilution techniques. The antibacterial potential was assessed against four food-borne pathogens: Staphylococcus aureus, Methicillin-resistant Staphylococcus aureus MRSA, Escherichia coli, Salmonella sp., and a soil bacterium, Bacillus subtilis. The results from the GC-MS analysis indicated a predominant presence of fatty alcohols, notably Stigmasta-3,5-diene 49.00, a steroid alcohol, and Tetradecen-11-yn-1-ol 35.37, a long-chain fatty alcohol. Additionally, lesser quantities of compounds such as E, E-7,11,15-Trimethyl-3-methylene-hexadeca-1,6,10,14-tetraene a diterpene at 9.68, Squalene a triterpene at 1.77, and 2,4,4-Trimethyl-3-hydroxymethyl-5a-3-methyl-but-2-enyl-cyclohexene a sesquiterpene at 4.19 were identified. The analysis showed that neem oil is rich in fatty alcohols and phytosterols, with lower terpenes and phenolic compounds. It showed no significant antibacterial activity against the tested bacteria. This suggests that from cold-press extraction, neem oil may not effectively combat food-borne pathogens and soil bacteria due to its fatty alcohol and phytosterol content, along with the bacterias resistance. Increasing the concentration of crude oil in the antibacterial test could lead to positive results. The findings indicate a need for further research to isolate stronger antibacterial molecules in neem oil by separating its components, focusing on extraction methods and solvent polarity.

Makale detayları

Chemical composition and antibacterial activity of Azadirachta indica seed oil from Chad

Yazarlar:

Brahim Mahamat Ousman | Omar Belmehdi

Yayıncı:

İzzet KARA

Ana Başlıklar:

Microbiology (Other)

Dergi:

International Journal of Secondary Metabolite Vol.12 Issue 2, pp.429-447

Belge Tipi:

Makale

Yayın Tarihi:

1/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001340464

pISSN:

2148-6905

Azadirachta indica

Neem oil

GC-MS

Antibacterial activity

ChadAzadirachta indica

Neem oil

GC-MS

Antibacterial activity

Chad

Sayı

Makale detayları

Yazarlar: Brahim Mahamat Ousman | Omar Belmehdi

Yayıncı: İzzet KARA

Ana Başlıklar: Microbiology (Other)

Dergi: International Journal of Secondary Metabolite Vol.12 Issue 2, pp.429-447

Belge Tipi: Makale

Yayın Tarihi: 1/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001340464

pISSN: 2148-6905

Sayı

Yazarlar:

Brahim Mahamat Ousman | Omar Belmehdi

Yayıncı:

İzzet KARA

Ana Başlıklar:

Microbiology (Other)

Dergi:

International Journal of Secondary Metabolite Vol.12 Issue 2, pp.429-447

Belge Tipi:

Makale

Yayın Tarihi:

1/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001340464

pISSN:

2148-6905