Antimycotic Potency of Lantana camara, Alchornea cordifolia, and Tabernaemontana pachysiphon against the Anthracnose Pathogen Colletotrichum destructivum

David Nwazuo Enyiukwu; Anderson Chidi Amadıoha

Makale detayları

Antimycotic Potency of Lantana camara, Alchornea cordifolia, and Tabernaemontana pachysiphon against the Anthracnose Pathogen Colletotrichum destructivum

Yazarlar:

David Nwazuo Enyiukwu | Anderson Chidi Amadıoha

Yayıncı:

Istanbul University

Ana Başlıklar:

Biochemistry and Cell Biology (Other)

Dergi:

European Journal of Biology Vol.84 Issue 1, pp.20-29

Belge Tipi:

Makale

Yayın Tarihi:

5/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001355096

pISSN:

2602-2575

Anahtar kelimeler:

Özet:

 Objective: Cowpeas are important components of tropical dishes. Attacks of anthracnose Colletotrichum destructivum cause huge yield losses on the crop. Antimicrobial resistance, and mammalian toxicity of many synthetic fungicides, have spurred search for alternative plant-based pesticides. This study therefore, assessed the antifungal effectiveness of fractions of Alchornea cordifolia, Lantana camara and Tabernaemontana pachysiphon against C. desructivum, and their phytochemical constituents. Materials and Methods: Methanol extracts of the test plant materials were fractionated in a column with solvents of varying polarity. Each fraction was tested against growth and spore germination of the pathogen using poison bait technique. The experiment was set up in a CRD consisting of 31 treatments replicated 3 times. The phytochemical determinations of the fractions were carried out using standard spectrophotometric analysis. Results: Fractions of L. camara, recorded the highest spore germination and radial growth inhibitions 54.25-73.13  and 60.12-78.81  followed by T. pachysiphon 29.16-62.12  and 32.93-66.05  and lastly, A. cordifolia 21.31-49.18  and 38.00-54.55  respectively. Generally, their fungitoxicity was L. camara  T. pachysiphon A. cordifolia. The total phenolic and tannins contents ranged from 623-980 and 313-529 GAEg of extract respectively, total alkaloids content 191-343 AEg of extract and total flavonoids contents were 106-701 mg ruting extract. Conclusion: The fractions of L. camara L₆₈, L₅₆, L₄₂ and L₄ and, T. pachysiphon fractions B₆₀, B₆₃ and B₈₇ demonstrated the highest levels of mycotoxicity. Hence, characterizing these fractions for eco-friendly antifungals in the control of C. destructivum is highly recommended to increase food-legume production.

 Objective: Cowpeas are important components of tropical dishes. Attacks of anthracnose Colletotrichum destructivum cause huge yield losses on the crop. Antimicrobial resistance, and mammalian toxicity of many synthetic fungicides, have spurred search for alternative plant-based pesticides. This study therefore, assessed the antifungal effectiveness of fractions of Alchornea cordifolia, Lantana camara and Tabernaemontana pachysiphon against C. desructivum, and their phytochemical constituents. Materials and Methods: Methanol extracts of the test plant materials were fractionated in a column with solvents of varying polarity. Each fraction was tested against growth and spore germination of the pathogen using poison bait technique. The experiment was set up in a CRD consisting of 31 treatments replicated 3 times. The phytochemical determinations of the fractions were carried out using standard spectrophotometric analysis. Results: Fractions of L. camara, recorded the highest spore germination and radial growth inhibitions 54.25-73.13  and 60.12-78.81  followed by T. pachysiphon 29.16-62.12  and 32.93-66.05  and lastly, A. cordifolia 21.31-49.18  and 38.00-54.55  respectively. Generally, their fungitoxicity was L. camara  T. pachysiphon A. cordifolia. The total phenolic and tannins contents ranged from 623-980 and 313-529 GAEg of extract respectively, total alkaloids content 191-343 AEg of extract and total flavonoids contents were 106-701 mg ruting extract. Conclusion: The fractions of L. camara L₆₈, L₅₆, L₄₂ and L₄ and, T. pachysiphon fractions B₆₀, B₆₃ and B₈₇ demonstrated the highest levels of mycotoxicity. Hence, characterizing these fractions for eco-friendly antifungals in the control of C. destructivum is highly recommended to increase food-legume production.

 Objective: Cowpeas are important components of tropical dishes. Attacks of anthracnose Colletotrichum destructivum cause huge yield losses on the crop. Antimicrobial resistance, and mammalian toxicity of many synthetic fungicides, have spurred search for alternative plant-based pesticides. This study therefore, assessed the antifungal effectiveness of fractions of Alchornea cordifolia, Lantana camara and Tabernaemontana pachysiphon against C. desructivum, and their phytochemical constituents. Materials and Methods: Methanol extracts of the test plant materials were fractionated in a column with solvents of varying polarity. Each fraction was tested against growth and spore germination of the pathogen using poison bait technique. The experiment was set up in a CRD consisting of 31 treatments replicated 3 times. The phytochemical determinations of the fractions were carried out using standard spectrophotometric analysis. Results: Fractions of L. camara, recorded the highest spore germination and radial growth inhibitions 54.25-73.13  and 60.12-78.81  followed by T. pachysiphon 29.16-62.12  and 32.93-66.05  and lastly, A. cordifolia 21.31-49.18  and 38.00-54.55  respectively. Generally, their fungitoxicity was L. camara  T. pachysiphon A. cordifolia. The total phenolic and tannins contents ranged from 623-980 and 313-529 GAEg of extract respectively, total alkaloids content 191-343 AEg of extract and total flavonoids contents were 106-701 mg ruting extract. Conclusion: The fractions of L. camara L₆₈, L₅₆, L₄₂ and L₄ and, T. pachysiphon fractions B₆₀, B₆₃ and B₈₇ demonstrated the highest levels of mycotoxicity. Hence, characterizing these fractions for eco-friendly antifungals in the control of C. destructivum is highly recommended to increase food-legume production.

 Objective: Cowpeas are important components of tropical dishes. Attacks of anthracnose Colletotrichum destructivum cause huge yield losses on the crop. Antimicrobial resistance, and mammalian toxicity of many synthetic fungicides, have spurred search for alternative plant-based pesticides. This study therefore, assessed the antifungal effectiveness of fractions of Alchornea cordifolia, Lantana camara and Tabernaemontana pachysiphon against C. desructivum, and their phytochemical constituents. Materials and Methods: Methanol extracts of the test plant materials were fractionated in a column with solvents of varying polarity. Each fraction was tested against growth and spore germination of the pathogen using poison bait technique. The experiment was set up in a CRD consisting of 31 treatments replicated 3 times. The phytochemical determinations of the fractions were carried out using standard spectrophotometric analysis. Results: Fractions of L. camara, recorded the highest spore germination and radial growth inhibitions 54.25-73.13  and 60.12-78.81  followed by T. pachysiphon 29.16-62.12  and 32.93-66.05  and lastly, A. cordifolia 21.31-49.18  and 38.00-54.55  respectively. Generally, their fungitoxicity was L. camara  T. pachysiphon A. cordifolia. The total phenolic and tannins contents ranged from 623-980 and 313-529 GAEg of extract respectively, total alkaloids content 191-343 AEg of extract and total flavonoids contents were 106-701 mg ruting extract. Conclusion: The fractions of L. camara L₆₈, L₅₆, L₄₂ and L₄ and, T. pachysiphon fractions B₆₀, B₆₃ and B₈₇ demonstrated the highest levels of mycotoxicity. Hence, characterizing these fractions for eco-friendly antifungals in the control of C. destructivum is highly recommended to increase food-legume production.

Makale detayları

Antimycotic Potency of Lantana camara, Alchornea cordifolia, and Tabernaemontana pachysiphon against the Anthracnose Pathogen Colletotrichum destructivum

Yazarlar:

David Nwazuo Enyiukwu | Anderson Chidi Amadıoha

Yayıncı:

Istanbul University

Ana Başlıklar:

Biochemistry and Cell Biology (Other)

Dergi:

European Journal of Biology Vol.84 Issue 1, pp.20-29

Belge Tipi:

Makale

Yayın Tarihi:

5/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001355096

pISSN:

2602-2575

Biopesticides

Colletotrichum destructivum

Lantana camara

Alchornea cordifolia

Tabernaemonta pachysiphon

Anthracnose

Sayı

Makale detayları

Antimycotic Potency of Lantana camara, Alchornea cordifolia, and Tabernaemontana pachysiphon against the Anthracnose Pathogen Colletotrichum destructivum

Yazarlar: David Nwazuo Enyiukwu | Anderson Chidi Amadıoha

Yayıncı: Istanbul University

Ana Başlıklar: Biochemistry and Cell Biology (Other)

Dergi: European Journal of Biology Vol.84 Issue 1, pp.20-29

Belge Tipi: Makale

Yayın Tarihi: 5/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001355096

pISSN: 2602-2575

Biopesticides

Colletotrichum destructivum

Lantana camara

Alchornea cordifolia

Tabernaemonta pachysiphon

Anthracnose

Sayı

Yazarlar:

David Nwazuo Enyiukwu | Anderson Chidi Amadıoha

Yayıncı:

Istanbul University

Ana Başlıklar:

Biochemistry and Cell Biology (Other)

Dergi:

European Journal of Biology Vol.84 Issue 1, pp.20-29

Belge Tipi:

Makale

Yayın Tarihi:

5/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001355096

pISSN:

2602-2575