Plant Regeneration from Unfertilized Ovaries of Sugar Beet ( Beta vulgaris L.)  Cultured In Vitro

Ekrem GÜREL

Makale detayları

Plant Regeneration from Unfertilized Ovaries of Sugar Beet ( Beta vulgaris L.) Cultured In Vitro

Yazarlar:

Ekrem GÜREL

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Botany 1998 Vol.22 Issue 4, pp.233-238

Belge Tipi:

Makale

Yayın Tarihi:

3/1998

Belge Dili:

Türkçe

Document ID (Org No.):

2000724871

Sıklık:

İki Aylık

eISSN:

1303-6106

pISSN:

1300-008X

Özet:

 

A. method is described for plant regeneration from unfertilized ovaries isolated from a diploid male sterile sugar beet  ( Beta vulgaris L.) breeding line that was developed at the Sugar Institute, Ankara, Turkey. Ovary explants were cultured on  Murashige & Skoog (MS) medium containing 2.0 mg/l benzylaminopurine (BAP). Two treatments were tested by incubating all of  the explants in darkness for 15 days, and then transferring one half to light and keeping the other half in darkness throughout the  culture. Callus formation occurred in both treatments more or less at similar rates: however, there was a significant difference  between the treatments with regard to the shoot-forming capacity of the explants, those transferred to light after an initial incbation  in darkness producing more shoots (14.6%) than those kept in darkness continuously (4.2%). Root induction was readilyachieved  within two weeks when shoots were transferred to MS medium supplemented with 2.0 mg/l naphthaleneacetic acid (NAA) and 2.0  mg/l silver nitrate (AgNO 3 ). An inverse relationship between the callus and shoot-forming capacity of the individual explants was  apparent. The determination of ploidy levels of the regenerants was performed by chromosome counting in leaf samples of  regenerated plants and the results revealed that all of the regenerants were diploid.

 

A. method is described for plant regeneration from unfertilized ovaries isolated from a diploid male sterile sugar beet  ( Beta vulgaris L.) breeding line that was developed at the Sugar Institute, Ankara, Turkey. Ovary explants were cultured on  Murashige & Skoog (MS) medium containing 2.0 mg/l benzylaminopurine (BAP). Two treatments were tested by incubating all of  the explants in darkness for 15 days, and then transferring one half to light and keeping the other half in darkness throughout the  culture. Callus formation occurred in both treatments more or less at similar rates: however, there was a significant difference  between the treatments with regard to the shoot-forming capacity of the explants, those transferred to light after an initial incbation  in darkness producing more shoots (14.6%) than those kept in darkness continuously (4.2%). Root induction was readilyachieved  within two weeks when shoots were transferred to MS medium supplemented with 2.0 mg/l naphthaleneacetic acid (NAA) and 2.0  mg/l silver nitrate (AgNO 3 ). An inverse relationship between the callus and shoot-forming capacity of the individual explants was  apparent. The determination of ploidy levels of the regenerants was performed by chromosome counting in leaf samples of  regenerated plants and the results revealed that all of the regenerants were diploid.

Makale detayları

Plant Regeneration from Unfertilized Ovaries of Sugar Beet ( Beta vulgaris L.) Cultured In Vitro

Yazarlar: Ekrem GÜREL

Yayıncı: TUBITAK

Dergi: Turkish Journal of Botany 1998 Vol.22 Issue 4, pp.233-238

Belge Tipi: Makale

Yayın Tarihi: 3/1998

Belge Dili: Türkçe

Document ID (Org No.): 2000724871

Sıklık: İki Aylık

eISSN: 1303-6106

pISSN: 1300-008X

Sayı

Yazarlar:

Ekrem GÜREL

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Botany 1998 Vol.22 Issue 4, pp.233-238

Belge Tipi:

Makale

Yayın Tarihi:

3/1998

Belge Dili:

Türkçe

Document ID (Org No.):

2000724871

Sıklık:

İki Aylık

eISSN:

1303-6106

pISSN:

1300-008X