Three Previously Unseen Genotypes Detected in IGFBP - 3 Gene in Buffalo Breed

Yusuf Özşensoy; İnanc Baral

Makale detayları

Three Previously Unseen Genotypes Detected in IGFBP - 3 Gene in Buffalo Breed

Yazarlar:

Yusuf Özşensoy | İnanc Baral

Yayıncı:

Harran University

Ana Başlıklar:

Zootekni | Genetik ve Biyoistatistik | Veteriner Cerrahi

Dergi:

Harran Üniversitesi Veteriner Fakültesi Dergisi Vol.12 Issue 1, pp.35-40

Belge Tipi:

Makale

Yayın Tarihi:

6/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001282259

pISSN:

2146-7188

Anahtar kelimeler:

Özet:

 Anatolian water buffalo breed is Turkeys sole water buffalo breed, and their numbers steadily increased with the national Water Buffalo Breeding by Breeders Project. This study aimed to investigate of the gene region polymorphisms Intron-2, Exon-2Intron-3, Exon-3 of the meat-yield-related Insulin-like Growth Factor Binding Protein-3 IGFBP-3 gene in Anatolian water buffaloes by TaqI, HaeIII, and MspI restriction endonucleases. The phenolchloroform method was used for DNA isolation from 151 blood samples, and extracted DNAs were amplified by touchdown PCR using specific primers. Amplified PCR products were digested with restriction endonucleases REs and separated in 3 agarose gel electrophoresis AGE, then genotypes were determined. Results revealed two genotypes AA 98.68 and AC 1.32 and two alleles A 0.99 and C 0.01 for the Exon-2 to Intron-3 region from HaeIII digestion. TaqI digestion of the Intron-2 region revealed three genotypes AA 7.94, AB 3.97, and BB 88.10 and two alleles A 0.10 and B 0.90. MspI digestion of the Exon-3 region revealed only the AA genotype and A allele, thus revealing monomorphism. Overall, HaeIII digestion revealed insignificant polymorphism P0.05, and TaqI digestion revealed significant polymorphism P0.001 for their respective regions. Gene polymorphisms of these regions were investigated for the first time in Anatolian water buffaloes. Additionally, three novel genotypes for the IGFBP-3 gene one from HaeIII and two from TaqI were determined for the first time. The novel B allele from TaqI digestion was observed to have a substantial frequency.

 Anatolian water buffalo breed is Turkeys sole water buffalo breed, and their numbers steadily increased with the national Water Buffalo Breeding by Breeders Project. This study aimed to investigate of the gene region polymorphisms Intron-2, Exon-2Intron-3, Exon-3 of the meat-yield-related Insulin-like Growth Factor Binding Protein-3 IGFBP-3 gene in Anatolian water buffaloes by TaqI, HaeIII, and MspI restriction endonucleases. The phenolchloroform method was used for DNA isolation from 151 blood samples, and extracted DNAs were amplified by touchdown PCR using specific primers. Amplified PCR products were digested with restriction endonucleases REs and separated in 3 agarose gel electrophoresis AGE, then genotypes were determined. Results revealed two genotypes AA 98.68 and AC 1.32 and two alleles A 0.99 and C 0.01 for the Exon-2 to Intron-3 region from HaeIII digestion. TaqI digestion of the Intron-2 region revealed three genotypes AA 7.94, AB 3.97, and BB 88.10 and two alleles A 0.10 and B 0.90. MspI digestion of the Exon-3 region revealed only the AA genotype and A allele, thus revealing monomorphism. Overall, HaeIII digestion revealed insignificant polymorphism P0.05, and TaqI digestion revealed significant polymorphism P0.001 for their respective regions. Gene polymorphisms of these regions were investigated for the first time in Anatolian water buffaloes. Additionally, three novel genotypes for the IGFBP-3 gene one from HaeIII and two from TaqI were determined for the first time. The novel B allele from TaqI digestion was observed to have a substantial frequency.

 Anatolian water buffalo breed is Turkeys sole water buffalo breed, and their numbers steadily increased with the national Water Buffalo Breeding by Breeders Project. This study aimed to investigate of the gene region polymorphisms Intron-2, Exon-2Intron-3, Exon-3 of the meat-yield-related Insulin-like Growth Factor Binding Protein-3 IGFBP-3 gene in Anatolian water buffaloes by TaqI, HaeIII, and MspI restriction endonucleases. The phenolchloroform method was used for DNA isolation from 151 blood samples, and extracted DNAs were amplified by touchdown PCR using specific primers. Amplified PCR products were digested with restriction endonucleases REs and separated in 3 agarose gel electrophoresis AGE, then genotypes were determined. Results revealed two genotypes AA 98.68 and AC 1.32 and two alleles A 0.99 and C 0.01 for the Exon-2 to Intron-3 region from HaeIII digestion. TaqI digestion of the Intron-2 region revealed three genotypes AA 7.94, AB 3.97, and BB 88.10 and two alleles A 0.10 and B 0.90. MspI digestion of the Exon-3 region revealed only the AA genotype and A allele, thus revealing monomorphism. Overall, HaeIII digestion revealed insignificant polymorphism P0.05, and TaqI digestion revealed significant polymorphism P0.001 for their respective regions. Gene polymorphisms of these regions were investigated for the first time in Anatolian water buffaloes. Additionally, three novel genotypes for the IGFBP-3 gene one from HaeIII and two from TaqI were determined for the first time. The novel B allele from TaqI digestion was observed to have a substantial frequency.

 Anadolu su mandası Türkiyede yetiştirilen tek manda ırkıdır ve sayıları ülke genelinde Halk Elinde Manda Yetiştiriciliği Projesi ile artmıştır. Bu çalışmanın amacı, Anadolu Mandalarında et verimi ile ilgili olan insülin benzeri büyüme faktörü bağlayıcı protein - 3 IGFBP-3 geninin Taq I, Hae III ve Msp I restriksiyon enzimi RE ile gen polimorfizmlerinin İntron 2, Exon 2  İntron 3, Exon 3 araştırılmasıdır. Toplam 151 kan örneğinden FenolKloroform yöntemi kullanılarak DNA izolasyonu yapılmıştır ve elde edilen DNA örnekleri spesifik primerler kullanılarak Touchdown PZR ile çoğaltılmıştır. Yükseltgenen PZR ürünleri restriksiyon enzimleri ile kesilmiş, 3 agaroz jel elektroforez yönteminde ayrıştırılmış ve genotipler belirlenmiştir. Sonuç olarak, Hae III REnde Exon 2  İntron 3 AA 98.68 ve AC 1.32 2 genotip ve A 0.99 ve C 0.01 2 allel tespit edilmiştir. Taq I REnde İntron 2 AA 7.94, AB 3.97 ve BB 88.10 3 genotip ve A 0.10 ve B 0.90 2 allel ve Msp I REnde Exon 3 ise sadece AA genotipi ve A alleli tespit edilmiştir. IGFBP-3 geninde Msp I enzimi monomorfik P0.05, Hae III enzimi polimorfik fakat önemsiz P0.05 bulunurken Taq 1 enzimi yönünden hem polimorfik hem de önemlilik tespit edilmiştir P0.001. IGFBP-3 gen bölgelerinin polimorfizmleri Anadolu mandalarında ilk kez araştırılmıştır. Ayrıca IGFBP-3 geninde diğer manda ırklarında yapılan çalışmalardan farklı olarak ilk kez üç yeni genotip Taq I enzim kesimi sonucunda iki, Hae III enzim kesimi sonucunda bir tespit edilmiştir. Ayrıca Taq I enzim işlemi sonucu yeni tespit edilen B allelinin frekansı diğer alleline göre çok yüksek bulunmuştur.

Makale detayları

Three Previously Unseen Genotypes Detected in IGFBP - 3 Gene in Buffalo Breed

Yazarlar:

Yusuf Özşensoy | İnanc Baral

Yayıncı:

Harran University

Ana Başlıklar:

Zootekni | Genetik ve Biyoistatistik | Veteriner Cerrahi

Dergi:

Harran Üniversitesi Veteriner Fakültesi Dergisi Vol.12 Issue 1, pp.35-40

Belge Tipi:

Makale

Yayın Tarihi:

6/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001282259

pISSN:

2146-7188

Anatolian water buffalo

HaeIII

IGFBP-3

PCR-RFLP

Sivas

TaqI

Anadolu Mandası

HaeIII

IGFBP-3

PZR-RFLP

Sivas

TaqI

Sayı

Makale detayları

Yazarlar: Yusuf Özşensoy | İnanc Baral

Yayıncı: Harran University

Ana Başlıklar: Zootekni | Genetik ve Biyoistatistik | Veteriner Cerrahi

Dergi: Harran Üniversitesi Veteriner Fakültesi Dergisi Vol.12 Issue 1, pp.35-40

Belge Tipi: Makale

Yayın Tarihi: 6/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001282259

pISSN: 2146-7188

Sayı

Yazarlar:

Yusuf Özşensoy | İnanc Baral

Yayıncı:

Harran University

Ana Başlıklar:

Zootekni | Genetik ve Biyoistatistik | Veteriner Cerrahi

Dergi:

Harran Üniversitesi Veteriner Fakültesi Dergisi Vol.12 Issue 1, pp.35-40

Belge Tipi:

Makale

Yayın Tarihi:

6/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001282259

pISSN:

2146-7188