EPD Coating for β-TCP/ Chitosan Using a Biomimetic Approach

Yasemin Tabak

Makale detayları

EPD Coating for β-TCP/ Chitosan Using a Biomimetic Approach

Yazarlar:

Yasemin Tabak

Yayıncı:

Giresun University

Ana Başlıklar:

Malzeme Üretim Teknolojileri

Dergi:

Karadeniz Fen Bilimleri Dergisi Vol.13 Issue 2, pp.347-362

Belge Tipi:

Makale

Yayın Tarihi:

6/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001290994

pISSN:

2564-7377

Anahtar kelimeler:

Özet:

 In this study coating trials are carried out on a functionally graded Si3N4 based ceramic substrate by electrophoretic deposition EPD method, for biomaterial applications. Coating solution is prepared in two stages, as Suspension 1 and Suspension 2, using tricalcium phosphate TCP, gelatine, chitosan, acetic acid, and water. Initially chitosan and acetic acid are mixed for 5 hours, and TCP and water are mixed for 4 hours. The latter is mixed for 1 more hour after addition of gelatine. Suspension 1 is formed by combining these two mixtures which is then mixed for 2 hours. On the other hand, Suspension 2 is prepared by mixing TCP, gelatine and water for 2 hours. The Si3N4 material is connected the negative terminal of the power supply as cathode and stainless steel material is connected to the positive terminal as anode. They are submerged into the coating solution as positioned parallel to each other. Coating process is carried out by applying 30 V potential between the electrodes that are kept 0,5 cm apart. Solution is continuously stirred during the process and three coating trials are carried out for 1, 2 and, 3 min. duration times. Coated samples are examined by scanning electron microscope SEM to investigate the effect of coating parameters on the coating thicknesses. X-ray diffraction XRD studies are performed to compare the coated and uncoated samples. Coated samples are kept in simulated body fluid SBF for 21 days and their SEM and XRD data revealed the achievement of biocompatible coatings. The effects of the coating were investigated by making surface roughness, contact angle and FTIR analyzes on the coated samples. As a result of the analyses made, it was seen that the sample with the best coating EPD-4 code.

 In this study coating trials are carried out on a functionally graded Si3N4 based ceramic substrate by electrophoretic deposition EPD method, for biomaterial applications. Coating solution is prepared in two stages, as Suspension 1 and Suspension 2, using tricalcium phosphate TCP, gelatine, chitosan, acetic acid, and water. Initially chitosan and acetic acid are mixed for 5 hours, and TCP and water are mixed for 4 hours. The latter is mixed for 1 more hour after addition of gelatine. Suspension 1 is formed by combining these two mixtures which is then mixed for 2 hours. On the other hand, Suspension 2 is prepared by mixing TCP, gelatine and water for 2 hours. The Si3N4 material is connected the negative terminal of the power supply as cathode and stainless steel material is connected to the positive terminal as anode. They are submerged into the coating solution as positioned parallel to each other. Coating process is carried out by applying 30 V potential between the electrodes that are kept 0,5 cm apart. Solution is continuously stirred during the process and three coating trials are carried out for 1, 2 and, 3 min. duration times. Coated samples are examined by scanning electron microscope SEM to investigate the effect of coating parameters on the coating thicknesses. X-ray diffraction XRD studies are performed to compare the coated and uncoated samples. Coated samples are kept in simulated body fluid SBF for 21 days and their SEM and XRD data revealed the achievement of biocompatible coatings. The effects of the coating were investigated by making surface roughness, contact angle and FTIR analyzes on the coated samples. As a result of the analyses made, it was seen that the sample with the best coating EPD-4 code.

 In this study coating trials are carried out on a functionally graded Si3N4 based ceramic substrate by electrophoretic deposition EPD method, for biomaterial applications. Coating solution is prepared in two stages, as Suspension 1 and Suspension 2, using tricalcium phosphate TCP, gelatine, chitosan, acetic acid, and water. Initially chitosan and acetic acid are mixed for 5 hours, and TCP and water are mixed for 4 hours. The latter is mixed for 1 more hour after addition of gelatine. Suspension 1 is formed by combining these two mixtures which is then mixed for 2 hours. On the other hand, Suspension 2 is prepared by mixing TCP, gelatine and water for 2 hours. The Si3N4 material is connected the negative terminal of the power supply as cathode and stainless steel material is connected to the positive terminal as anode. They are submerged into the coating solution as positioned parallel to each other. Coating process is carried out by applying 30 V potential between the electrodes that are kept 0,5 cm apart. Solution is continuously stirred during the process and three coating trials are carried out for 1, 2 and, 3 min. duration times. Coated samples are examined by scanning electron microscope SEM to investigate the effect of coating parameters on the coating thicknesses. X-ray diffraction XRD studies are performed to compare the coated and uncoated samples. Coated samples are kept in simulated body fluid SBF for 21 days and their SEM and XRD data revealed the achievement of biocompatible coatings. The effects of the coating were investigated by making surface roughness, contact angle and FTIR analyzes on the coated samples. As a result of the analyses made, it was seen that the sample with the best coating EPD-4 code.

 Bu çalışmada, biyomalzeme uygulamalarında kullanılmak üzere, elektroforetik kaplama yöntemi EPD ile fonksiyonel derecelendirilmiş Si3N4 esaslı seramik altlık üzerine kaplama denemeleri yapılmıştır. Kaplama çözeltisi trikalsiyum fosfat TCP, jelatin, kitosan, asetik asit ve su ile hazırlanmıştır. Çözeltinin hazırlanması Süspansiyon-1 ve Süspansiyon-2nin hazırlanması şeklinde iki aşamada gerçekleştirilmiştir. İlk olarak kitosan ve asetik asit 5 saat boyunca karıştırılmıştır. Buna paralel olarak TCP ve su 4 saat boyunca karıştırılmış, TCP ve su karışımına jelatin ilave edildikten sonra 1 saat daha karıştırılmıştır. Hazırlanan bu iki karışım birleştirilerek Süspansiyon 1 oluşturulmuştur. Ardından Süspansiyon 1 karışımı 2 saat boyunca karıştırılmıştır. Süspansiyon 2nin hazırlanması için ise TCP, jelatin ve su karışımı 2 saat boyunca karıştırılmıştır. Güç kaynağının katot - kutbuna bağlanmış olan Si3N4 malzeme ve anot kutbuna  bağlanmış olan paslanmaz çelik malzeme, birbirlerine paralel duracak şekilde kaplama çözeltisi içerisine daldırılmıştır. Sonrasında güç kaynağından 30 V voltaj uygulanarak kaplama uygulaması yapılmıştır. Kaplama esnasında çözeltilerin karıştırılmasına devam edilmiş olup; 3 farklı denemede hazırlanan kaplama uygulamalarında elektrotlar arası mesafe 0,5 cm, uygulama süresi sırasıyla 1 dk, 2dk ve 3dk olarak denenmiştir. Kaplanan numunelerin taramalı elektron mikroskobu SEM ile yapılan mikroyapı analizlerinde uygulama esnasındaki parametrelerin kaplama kalınlıklarına etkisi incelenmiştir. X-ışını difraksiyonu XRD ile kaplanmış ve kaplanmamış numuneler karşılaştırılmıştır. Yapay vücut sıvısı SBF içerisinde 21 gün bekletilen kaplanmış numunelerin SEM ve XRD analiz sonuçları irdelendiğinde biyouyumlu kaplamaların geliştirildiği görülmüştür. Kaplamalı numunelere yüzey pürüzlüğü, temas açısı ve FTIR analizleri yapılarak kaplamanın etkileri incelenmiştir. Yapılan analizler sonucu en iyi kaplama EPD-4 koduna sahip numune olduğu görülmüştür.

Makale detayları

EPD Coating for β-TCP/ Chitosan Using a Biomimetic Approach

Yazarlar:

Yasemin Tabak

Yayıncı:

Giresun University

Ana Başlıklar:

Malzeme Üretim Teknolojileri

Dergi:

Karadeniz Fen Bilimleri Dergisi Vol.13 Issue 2, pp.347-362

Belge Tipi:

Makale

Yayın Tarihi:

6/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001290994

pISSN:

2564-7377

Electrophoretic deposition

Silicon nitride

TCP

ChitosanElektroforetik kaplama

Silisyum nitrür

TCP

Kitosan

Sayı

Makale detayları

Yazarlar: Yasemin Tabak

Yayıncı: Giresun University

Ana Başlıklar: Malzeme Üretim Teknolojileri

Dergi: Karadeniz Fen Bilimleri Dergisi Vol.13 Issue 2, pp.347-362

Belge Tipi: Makale

Yayın Tarihi: 6/2023

Belge Dili: Türkçe

Document ID (Org No.): 2001290994

pISSN: 2564-7377

Sayı

Yazarlar:

Yasemin Tabak

Yayıncı:

Giresun University

Ana Başlıklar:

Malzeme Üretim Teknolojileri

Dergi:

Karadeniz Fen Bilimleri Dergisi Vol.13 Issue 2, pp.347-362

Belge Tipi:

Makale

Yayın Tarihi:

6/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001290994

pISSN:

2564-7377