Production of Monoclonal Antibodies Specific for  Progesterone, Estradiole by Simultaneous Injection  of Different Steroids

Fatima Şahingöz YÜCEL

Makale detayları

Production of Monoclonal Antibodies Specific for Progesterone, Estradiole by Simultaneous Injection of Different Steroids

Yazarlar:

Fatima Şahingöz YÜCEL

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2000 Vol.24 Issue 4, pp.697-706

Belge Tipi:

Makale

Yayın Tarihi:

3/2000

Belge Dili:

Türkçe

Document ID (Org No.):

2000813919

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Anahtar kelimeler:

Özet:

 

We report here the development of hybrid cells producing monoclonal antibodies specific for  two different steroid hormones with mixed immunization using hybridoma technology. BALB/c mice  were immunized with a mixture of three steroid antigens: progesterone, estradiole and testosterone  linked to bovine serum albumine. These mice were used for fusion. In the two fusion experiments,  ELISA tests showed that among 645 wells only 2 hybrids reacted with progesterone (MAM 3C2, MAM  3E3) and one of them reacted specifically with estradiol (MAM 7H7). Three types of monoclonal  antibodies were detected: IgG1, IgG2a and IgG2b and the affinities of these antibodies were found  to be 10 -8 -10 -9 M. Monoclonal antibodies did not cross react with the other steroid hormones.

 

We report here the development of hybrid cells producing monoclonal antibodies specific for  two different steroid hormones with mixed immunization using hybridoma technology. BALB/c mice  were immunized with a mixture of three steroid antigens: progesterone, estradiole and testosterone  linked to bovine serum albumine. These mice were used for fusion. In the two fusion experiments,  ELISA tests showed that among 645 wells only 2 hybrids reacted with progesterone (MAM 3C2, MAM  3E3) and one of them reacted specifically with estradiol (MAM 7H7). Three types of monoclonal  antibodies were detected: IgG1, IgG2a and IgG2b and the affinities of these antibodies were found  to be 10 -8 -10 -9 M. Monoclonal antibodies did not cross react with the other steroid hormones.

Makale detayları

Production of Monoclonal Antibodies Specific for Progesterone, Estradiole by Simultaneous Injection of Different Steroids

Yazarlar:

Fatima Şahingöz YÜCEL

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2000 Vol.24 Issue 4, pp.697-706

Belge Tipi:

Makale

Yayın Tarihi:

3/2000

Belge Dili:

Türkçe

Document ID (Org No.):

2000813919

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Immunodiagnosis

hybrid cell

monoclonal antibody

steroid hormones

ELISA.

Sayı

Makale detayları

Production of Monoclonal Antibodies Specific for Progesterone, Estradiole by Simultaneous Injection of Different Steroids

Yazarlar: Fatima Şahingöz YÜCEL

Yayıncı: TUBITAK

Dergi: Turkish Journal of Biology 2000 Vol.24 Issue 4, pp.697-706

Belge Tipi: Makale

Yayın Tarihi: 3/2000

Belge Dili: Türkçe

Document ID (Org No.): 2000813919

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

Immunodiagnosis

hybrid cell

monoclonal antibody

steroid hormones

ELISA.

Sayı

Yazarlar:

Fatima Şahingöz YÜCEL

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2000 Vol.24 Issue 4, pp.697-706

Belge Tipi:

Makale

Yayın Tarihi:

3/2000

Belge Dili:

Türkçe

Document ID (Org No.):

2000813919

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152