Development of short gSSRs in G. arboreum and their utilization in phylogenetic studies

Tayyaba SHAHEEN; Yusuf ZAFAR; James M. STEWART; Mehboob-ur RAHMAN

Makale detayları

Development of short gSSRs in G. arboreum and their utilization in phylogenetic studies

Yazarlar:

Tayyaba SHAHEEN | Yusuf ZAFAR | James M. STEWART | Mehboob-ur RAHMAN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Agriculture and Forestry 2013 Vol.37 Issue 3, pp.288-299

Belge Tipi:

Makale

Yayın Tarihi:

2/2013

Belge Dili:

Türkçe

Document ID (Org No.):

2000807661

Sıklık:

İki Aylık

eISSN:

1303-6173

pISSN:

1300-011X

Anahtar kelimeler:

Özet:

 

Microsatellite regions in DNA fragments of Gossypium arboreum were explored and PCR products 500-800 bp long were analyzed for genomic simple sequence repeats (gSSRs). From 39 segments, 23 short gSSRs were identified; on average they occurred every 1.6 kb. Primers were used to amplify fragments from 18 diploid species representing 6 diploid genomes and 1 tetraploid species, G. hirsutum (AD). The phylogeny of diploid genomes was subsequently determined. No primer amplified the DNA from all genotypes; however, amplification occurred in 59% of 437 PCR events. All genomes were grouped into 2 clusters (a and b). The obtained phylogeny of the species parallels previous reports. The strategy adopted here is an efficient method of identifying new gSSRs from species not extensively explored.

 

Microsatellite regions in DNA fragments of Gossypium arboreum were explored and PCR products 500-800 bp long were analyzed for genomic simple sequence repeats (gSSRs). From 39 segments, 23 short gSSRs were identified; on average they occurred every 1.6 kb. Primers were used to amplify fragments from 18 diploid species representing 6 diploid genomes and 1 tetraploid species, G. hirsutum (AD). The phylogeny of diploid genomes was subsequently determined. No primer amplified the DNA from all genotypes; however, amplification occurred in 59% of 437 PCR events. All genomes were grouped into 2 clusters (a and b). The obtained phylogeny of the species parallels previous reports. The strategy adopted here is an efficient method of identifying new gSSRs from species not extensively explored.

Makale detayları

Development of short gSSRs in G. arboreum and their utilization in phylogenetic studies

Yazarlar:

Tayyaba SHAHEEN | Yusuf ZAFAR | James M. STEWART | Mehboob-ur RAHMAN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Agriculture and Forestry 2013 Vol.37 Issue 3, pp.288-299

Belge Tipi:

Makale

Yayın Tarihi:

2/2013

Belge Dili:

Türkçe

Document ID (Org No.):

2000807661

Sıklık:

İki Aylık

eISSN:

1303-6173

pISSN:

1300-011X

Key words: Gossypium

phylogenetic assessment

RAPD

short gSSRs

Sayı

Makale detayları

Development of short gSSRs in G. arboreum and their utilization in phylogenetic studies

Yazarlar: Tayyaba SHAHEEN | Yusuf ZAFAR | James M. STEWART | Mehboob-ur RAHMAN

Yayıncı: TUBITAK

Dergi: Turkish Journal of Agriculture and Forestry 2013 Vol.37 Issue 3, pp.288-299

Belge Tipi: Makale

Yayın Tarihi: 2/2013

Belge Dili: Türkçe

Document ID (Org No.): 2000807661

Sıklık: İki Aylık

eISSN: 1303-6173

pISSN: 1300-011X

Key words: Gossypium

phylogenetic assessment

RAPD

short gSSRs

Sayı

Yazarlar:

Tayyaba SHAHEEN | Yusuf ZAFAR | James M. STEWART | Mehboob-ur RAHMAN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Agriculture and Forestry 2013 Vol.37 Issue 3, pp.288-299

Belge Tipi:

Makale

Yayın Tarihi:

2/2013

Belge Dili:

Türkçe

Document ID (Org No.):

2000807661

Sıklık:

İki Aylık

eISSN:

1303-6173

pISSN:

1300-011X