Rhizogenesis in Shrub rose cultivated in vitro

Alla Konopelko

Makale detayları

Rhizogenesis in Shrub rose cultivated in vitro

Yazarlar:

Alla Konopelko

Yayıncı:

Trakya University

Ana Başlıklar:

Plant Biotechnology

Dergi:

Trakya University Journal of Natural Sciences Vol.25 Issue 2, pp.187-196

Belge Tipi:

Makale

Yayın Tarihi:

10/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001324500

pISSN:

2528-9691

Anahtar kelimeler:

Özet:

 The study of the reproduction characteristics of roses of the garden class Shrub, the defini-tion of the dependence of the hormonal determination of explant rhizogenesis on the con-centrations of phytohormones that are part of the nutrient medium, are relevant and has both scientific and practical interest. This study presents the results of studies of hormonal de-termination of rhizogenesis in explants of cultivars of roses of the garden class Shrub: Gärt-nerfreude, Lavender Dream, Pomponella, Red Cascade, Sommerabend cultivated in vitro on nutrient medium containing growth regulators. It has been established that of the nutrient medium modified by the addition of 0.2-1.0 mgl α-naphthylacetic acid α-NAA, the most effective was the medium with the content of α-NAA 0.5 mgl, the content of macro- and microelements half of the Murashige and Skoog prescriptions, and a decrease in the sucrose content to 2.0. On this medium, the frequency of rhizogenesis averaged 61.2 for the studied cultivars. Hormonal determination of rhizogenesis and efficiency of root formation in vitro in the Shrub rose regenerants depended on the genotype of the plant: cv. Lavender Dream 66.0 and cv. Sommerabend 67.0 had the highest rhizogenesis ability. The use of the universal growth regulator Humifield in combination with 0.5 mgl α-NAA contribut-ed to an increase in the rooting rate of the studied rose cultivars up to 70.0-86.0.

 The study of the reproduction characteristics of roses of the garden class Shrub, the defini-tion of the dependence of the hormonal determination of explant rhizogenesis on the con-centrations of phytohormones that are part of the nutrient medium, are relevant and has both scientific and practical interest. This study presents the results of studies of hormonal de-termination of rhizogenesis in explants of cultivars of roses of the garden class Shrub: Gärt-nerfreude, Lavender Dream, Pomponella, Red Cascade, Sommerabend cultivated in vitro on nutrient medium containing growth regulators. It has been established that of the nutrient medium modified by the addition of 0.2-1.0 mgl α-naphthylacetic acid α-NAA, the most effective was the medium with the content of α-NAA 0.5 mgl, the content of macro- and microelements half of the Murashige and Skoog prescriptions, and a decrease in the sucrose content to 2.0. On this medium, the frequency of rhizogenesis averaged 61.2 for the studied cultivars. Hormonal determination of rhizogenesis and efficiency of root formation in vitro in the Shrub rose regenerants depended on the genotype of the plant: cv. Lavender Dream 66.0 and cv. Sommerabend 67.0 had the highest rhizogenesis ability. The use of the universal growth regulator Humifield in combination with 0.5 mgl α-NAA contribut-ed to an increase in the rooting rate of the studied rose cultivars up to 70.0-86.0.

 The study of the reproduction characteristics of roses of the garden class Shrub, the defini-tion of the dependence of the hormonal determination of explant rhizogenesis on the con-centrations of phytohormones that are part of the nutrient medium, are relevant and has both scientific and practical interest. This study presents the results of studies of hormonal de-termination of rhizogenesis in explants of cultivars of roses of the garden class Shrub: Gärt-nerfreude, Lavender Dream, Pomponella, Red Cascade, Sommerabend cultivated in vitro on nutrient medium containing growth regulators. It has been established that of the nutrient medium modified by the addition of 0.2-1.0 mgl α-naphthylacetic acid α-NAA, the most effective was the medium with the content of α-NAA 0.5 mgl, the content of macro- and microelements half of the Murashige and Skoog prescriptions, and a decrease in the sucrose content to 2.0. On this medium, the frequency of rhizogenesis averaged 61.2 for the studied cultivars. Hormonal determination of rhizogenesis and efficiency of root formation in vitro in the Shrub rose regenerants depended on the genotype of the plant: cv. Lavender Dream 66.0 and cv. Sommerabend 67.0 had the highest rhizogenesis ability. The use of the universal growth regulator Humifield in combination with 0.5 mgl α-NAA contribut-ed to an increase in the rooting rate of the studied rose cultivars up to 70.0-86.0.

 Çalı güllerinin üreme özelliklerinin incelenmesi ve eksplant kök oluşumunun hormonal kontrolünün kullanılan besleyici ortamın fitohormon konsantrasyonuna bağlı olduğunun tanımlanması önemlidir ve hem bilimsel hem de pratik açıdan üzerinde durulan konulardır. Bu çalışmada, büyüme düzenleyicileri içeren besin ortamı üzerinde in vitro olarak yetiştiri-len çalı gülü kültivarlarının Gärtnerfreude, Lavender Dream, Pomponella, Red Cascade, Sommerabend eksplantlarında kök oluşumunun hormonal belirlenmesine yönelik deneme-lerin sonuçları sunulmuştur. 0,2-1,0 mgl a-naftilasetik asit a-NAA ilavesiyle modifiye edilen besin ortamlarından en etkilisinin, 0,5 mgl a-NAA içerikli, sukroz oranı 2ye kadar düşürülmüş ortam olduğu tespit edilmiştir. Bu ortamda, çalışılan kültivarlar için kök oluşu-mu sıklığı ortalama 61,2 olarak belirlenmiştir. Kullanılan kültivarlardaki kök oluşumunun in vitro etkinliğindeki hormonal katkının kültivarların genotiplerine bağlı olduğu belirlen-miştir: Lavender Dream kültivarı 66,0 ve Sommerabend kültivarı 67,0 en yüksekkök oluşumu yeteneği sergilemişlerdir. Bir büyüme düzenleyicisi olan Humifieldin 0,5 mgl α-NAA ile kombinasyon halinde kullanılması, incelenen gül kültvarlarında köklenme oranının 70,0-86,0a kadar artmasına katkıda bulunmuştur.

Makale detayları

Rhizogenesis in Shrub rose cultivated in vitro

Yazarlar:

Alla Konopelko

Yayıncı:

Trakya University

Ana Başlıklar:

Plant Biotechnology

Dergi:

Trakya University Journal of Natural Sciences Vol.25 Issue 2, pp.187-196

Belge Tipi:

Makale

Yayın Tarihi:

10/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001324500

pISSN:

2528-9691

micropropagation

roses

phytohormones

nutrient medium

determination

rooting

Sayı

Makale detayları

Rhizogenesis in Shrub rose cultivated in vitro

Yazarlar: Alla Konopelko

Yayıncı: Trakya University

Ana Başlıklar: Plant Biotechnology

Dergi: Trakya University Journal of Natural Sciences Vol.25 Issue 2, pp.187-196

Belge Tipi: Makale

Yayın Tarihi: 10/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001324500

pISSN: 2528-9691

micropropagation

roses

phytohormones

nutrient medium

determination

rooting

Sayı

Yazarlar:

Alla Konopelko

Yayıncı:

Trakya University

Ana Başlıklar:

Plant Biotechnology

Dergi:

Trakya University Journal of Natural Sciences Vol.25 Issue 2, pp.187-196

Belge Tipi:

Makale

Yayın Tarihi:

10/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001324500

pISSN:

2528-9691