Osajin is a promising candidate for sepsis-induced brain damage via suppression of the 8-OHdG/Bax/Caspase-3 pathway in a rat model of sepsis

Mohammad Alhilal; Serkan Yıldırım; Hüseyin Serkan Erol; Suzan Alhilal; Metin Kılıçlıoğlu; Berrah Gözegir; Murat Koç; Mesut Bünyami Halıcı

Makale detayları

Osajin is a promising candidate for sepsis-induced brain damage via suppression of the 8-OHdG/Bax/Caspase-3 pathway in a rat model of sepsis

Yazarlar:

Mohammad Alhilal | Serkan Yıldırım | Hüseyin Serkan Erol | Suzan Alhilal | Metin Kılıçlıoğlu | Berrah Gözegir | Murat Koç | Mesut Bünyami Halıcı

Yayıncı:

MediHealth Academy Yayıncılık

Ana Başlıklar:

Eczacılık Biyokimyası | Klinik Kimya | Patoloji

Dergi:

Journal of Health Sciences and Medicine Vol.8 Issue 2, pp.191-196

Belge Tipi:

Makale

Yayın Tarihi:

3/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001343100

pISSN:

2636-8579

Anahtar kelimeler:

Özet:

 Aims: We examined the protective effect of the natural product osajin against sepsis-induced brain damage by targeting the 8-hydroxydeoxyguanosine 8-OHdGBcl-2-associatedprotein Baxcaspase-3 pathway in the brain tissue of septic rats. Methods: Osajin was isolated from Maclura pomifera fruit, the structure was confirmed, and a rat model of brain damage was established by the cecal ligation and puncture CLP method. Osajin was administered to the animals with sepsis-associated brain damage at 150 and 300 mgkg. Following euthanasia, histopathological examination, detection of 8-OHdG by immunohistochemistry, and the estimation of Bax and caspase-3 expression using an immunofluorescent technique in the brain tissue were performed. Results: Histopathological examination revealed the presence of severe inflammation, marked degeneration, and necrosis in the brains of rats with sepsis. The results of immunohistochemical and immunofluorescent assays revealed that the CLP technique induced marked 8-OHdG, Bax, and caspase-3 expression in the brain tissues of septic rats compared with those in healthy rats. Osajin administration at a dose of 150 mgkg p0.05 and 300 mgkg p0.0022 reversed the histopathological changes and significantly ameliorated the increased 8-OHdG, Bax, and caspase-3 expression compared with that in septic rats. Conclusion: The histopathological, immunohistochemical, and immunofluorescent evidence indicates that osajin can reverse brain damage caused by sepsis by inhibiting the 8-OHdGBaxcaspase-3 pathway. Accordingly, this natural product represents a promising candidate for the management of brain damage in septic patients.

 Aims: We examined the protective effect of the natural product osajin against sepsis-induced brain damage by targeting the 8-hydroxydeoxyguanosine 8-OHdGBcl-2-associatedprotein Baxcaspase-3 pathway in the brain tissue of septic rats. Methods: Osajin was isolated from Maclura pomifera fruit, the structure was confirmed, and a rat model of brain damage was established by the cecal ligation and puncture CLP method. Osajin was administered to the animals with sepsis-associated brain damage at 150 and 300 mgkg. Following euthanasia, histopathological examination, detection of 8-OHdG by immunohistochemistry, and the estimation of Bax and caspase-3 expression using an immunofluorescent technique in the brain tissue were performed. Results: Histopathological examination revealed the presence of severe inflammation, marked degeneration, and necrosis in the brains of rats with sepsis. The results of immunohistochemical and immunofluorescent assays revealed that the CLP technique induced marked 8-OHdG, Bax, and caspase-3 expression in the brain tissues of septic rats compared with those in healthy rats. Osajin administration at a dose of 150 mgkg p0.05 and 300 mgkg p0.0022 reversed the histopathological changes and significantly ameliorated the increased 8-OHdG, Bax, and caspase-3 expression compared with that in septic rats. Conclusion: The histopathological, immunohistochemical, and immunofluorescent evidence indicates that osajin can reverse brain damage caused by sepsis by inhibiting the 8-OHdGBaxcaspase-3 pathway. Accordingly, this natural product represents a promising candidate for the management of brain damage in septic patients.

 Aims: We examined the protective effect of the natural product osajin against sepsis-induced brain damage by targeting the 8-hydroxydeoxyguanosine 8-OHdGBcl-2-associatedprotein Baxcaspase-3 pathway in the brain tissue of septic rats. Methods: Osajin was isolated from Maclura pomifera fruit, the structure was confirmed, and a rat model of brain damage was established by the cecal ligation and puncture CLP method. Osajin was administered to the animals with sepsis-associated brain damage at 150 and 300 mgkg. Following euthanasia, histopathological examination, detection of 8-OHdG by immunohistochemistry, and the estimation of Bax and caspase-3 expression using an immunofluorescent technique in the brain tissue were performed. Results: Histopathological examination revealed the presence of severe inflammation, marked degeneration, and necrosis in the brains of rats with sepsis. The results of immunohistochemical and immunofluorescent assays revealed that the CLP technique induced marked 8-OHdG, Bax, and caspase-3 expression in the brain tissues of septic rats compared with those in healthy rats. Osajin administration at a dose of 150 mgkg p0.05 and 300 mgkg p0.0022 reversed the histopathological changes and significantly ameliorated the increased 8-OHdG, Bax, and caspase-3 expression compared with that in septic rats. Conclusion: The histopathological, immunohistochemical, and immunofluorescent evidence indicates that osajin can reverse brain damage caused by sepsis by inhibiting the 8-OHdGBaxcaspase-3 pathway. Accordingly, this natural product represents a promising candidate for the management of brain damage in septic patients.

 Amaçlar: Doğal ürün olan osajinin, sepsis kaynaklı beyin hasarına karşı koruyucu etkisini, sepsisli sıçanların beyin dokusundaki 8-hidroksideoksiguanozin 8-OHdGBcl-2 ile ilişkili  protein Baxkaspaz-3 yolunu hedef alarak inceledik. Metotlar: Osajin, Maclura pomiferanın meyvesinden izole edilip yapısı doğrulandı. Çekal ligasyon-punksiyon CLP yöntemi ile sıçanlarda beyin hasarı modeli oluşturuldu. Osajin, sepsise bağlı beyin hasarı olan hayvanlara 150 ve 300 mgkg dozlarda uygulanmıştır. Ötenazi sonrasında histopatolojik inceleme, immünohistokimya ile 8-OHdGnin tespiti ve immünfloresan teknik kullanılarak Bax ve kaspaz-3 ekspresyonunun tahmini beyin dokusunda yapıldı. Bulgular: Histopatolojik incelemede sepsisli sıçanların beyinlerinde şiddetli düzeyde inflamasyon, belirgin dejenerasyon ve nekroz görüldü. İmmünohistokimyasal ve immünfloresan analizlerin bulguları, CLP tekniğinin, sağlıklı sıçanlarla karşılaştırıldığında sepsisli sıçanların beyin dokularında belirgin 8-OHdG, Bax ve kaspaz-3 ekspresyonunu indüklediğini ortaya çıkardı. 150 mgkg p  0.05 ve 300 mgkg p  0.0022 dozlarda osajinin uygulanması, histopatolojik değişiklikleri tersine çevirdiği ve sepsisli sıçanlarla kıyaslandığında artan 8-OHdG, Bax ve kaspaz-3 ekspresyonunu önemli ölçüde iyileştirdiği gözlendi. Sonuç: Histopatolojik, immünohistokimyasal ve immünfloresan kanıtlar, osajinin, 8-OHdGBaxkaspaz-3 yolunu inhibe ederek sepsisin neden olduğu beyin hasarını tersine çevirebileceğini göstermektedir. Buna göre bu doğal ürünün, sepsisli hastalardaki beyin hasarının tedavisi için umut verici bir aday olabileceği gösterilmiştir.

Makale detayları

Osajin is a promising candidate for sepsis-induced brain damage via suppression of the 8-OHdG/Bax/Caspase-3 pathway in a rat model of sepsis

Yazarlar:

Mohammad Alhilal | Serkan Yıldırım | Hüseyin Serkan Erol | Suzan Alhilal | Metin Kılıçlıoğlu | Berrah Gözegir | Murat Koç | Mesut Bünyami Halıcı

Yayıncı:

MediHealth Academy Yayıncılık

Ana Başlıklar:

Eczacılık Biyokimyası | Klinik Kimya | Patoloji

Dergi:

Journal of Health Sciences and Medicine Vol.8 Issue 2, pp.191-196

Belge Tipi:

Makale

Yayın Tarihi:

3/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001343100

pISSN:

2636-8579

Bax

caspase-3

osajin

sepsis

brain damage

8-OHdGsBax

Kaspaz-3

Osajin

Sepsis

Beyin hasarı

8-OHdG

Sayı

Makale detayları

Yazarlar: Mohammad Alhilal | Serkan Yıldırım | Hüseyin Serkan Erol | Suzan Alhilal | Metin Kılıçlıoğlu | Berrah Gözegir | Murat Koç | Mesut Bünyami Halıcı

Yayıncı: MediHealth Academy Yayıncılık

Ana Başlıklar: Eczacılık Biyokimyası | Klinik Kimya | Patoloji

Dergi: Journal of Health Sciences and Medicine Vol.8 Issue 2, pp.191-196

Belge Tipi: Makale

Yayın Tarihi: 3/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001343100

pISSN: 2636-8579

Sayı

Yazarlar:

Mohammad Alhilal | Serkan Yıldırım | Hüseyin Serkan Erol | Suzan Alhilal | Metin Kılıçlıoğlu | Berrah Gözegir | Murat Koç | Mesut Bünyami Halıcı

Yayıncı:

MediHealth Academy Yayıncılık

Ana Başlıklar:

Eczacılık Biyokimyası | Klinik Kimya | Patoloji

Dergi:

Journal of Health Sciences and Medicine Vol.8 Issue 2, pp.191-196

Belge Tipi:

Makale

Yayın Tarihi:

3/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001343100

pISSN:

2636-8579