The effects of adding methylprednisolone to low-potassium dextran solution on preserved ischemic lungs

Ali YEGİNSU; Makbule ERGİN; Hüseyin ÖZYURT; Çiğdem ELMAS; Güleser ÇAĞLAR GÖKTAŞ

Makale detayları

The effects of adding methylprednisolone to low-potassium dextran solution on preserved ischemic lungs

Yazarlar:

Ali YEGİNSU | Makbule ERGİN | Hüseyin ÖZYURT | Çiğdem ELMAS | Güleser ÇAĞLAR GÖKTAŞ

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2010 Vol.40 Issue 1, pp.39-46

Belge Tipi:

Makale

Yayın Tarihi:

12/2009

Belge Dili:

Türkçe

Document ID (Org No.):

2000755189

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Anahtar kelimeler:

Özet:

 

To investigate the effects of adding methylprednisolone to low-potassium dextran solution on cold-preserved ischemic lungs.   Materials and methods: The study included 42 rat lung blocks that were divided into 7 groups (n = 6). Lung blocks were preserved in 40 mL of cold Perfadex solution, to which 5, 15, 30, 60, and 120 mg kg-1 (approximately 40, 120, 240, 480, and 960 mg L-1) of methylprednisolone were added. After a 24-h preservation period, the lungs were removed from the solution and examined.   Results: As compared to the Perfadex only group, cell death and degeneration were significantly higher in the Perfadex + methylprednisolone groups (P < 0.05). MDA and IL-8 levels were higher in all Perfadex + methylprednisolone groups. Statistically significant differences were observed between some of these groups. Antioxidant enzyme activity was generally higher in the Perfadex + methylprednisolone groups than in the Perfadex only group (P < 0.05).  Conclusion: Adding methylprednisolone to low-potassium dextran solution does not have any beneficial effects but has possible deleterious effects in rat lung preservation.

 

To investigate the effects of adding methylprednisolone to low-potassium dextran solution on cold-preserved ischemic lungs.   Materials and methods: The study included 42 rat lung blocks that were divided into 7 groups (n = 6). Lung blocks were preserved in 40 mL of cold Perfadex solution, to which 5, 15, 30, 60, and 120 mg kg-1 (approximately 40, 120, 240, 480, and 960 mg L-1) of methylprednisolone were added. After a 24-h preservation period, the lungs were removed from the solution and examined.   Results: As compared to the Perfadex only group, cell death and degeneration were significantly higher in the Perfadex + methylprednisolone groups (P < 0.05). MDA and IL-8 levels were higher in all Perfadex + methylprednisolone groups. Statistically significant differences were observed between some of these groups. Antioxidant enzyme activity was generally higher in the Perfadex + methylprednisolone groups than in the Perfadex only group (P < 0.05).  Conclusion: Adding methylprednisolone to low-potassium dextran solution does not have any beneficial effects but has possible deleterious effects in rat lung preservation.

Makale detayları

The effects of adding methylprednisolone to low-potassium dextran solution on preserved ischemic lungs

Yazarlar:

Ali YEGİNSU | Makbule ERGİN | Hüseyin ÖZYURT | Çiğdem ELMAS | Güleser ÇAĞLAR GÖKTAŞ

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2010 Vol.40 Issue 1, pp.39-46

Belge Tipi:

Makale

Yayın Tarihi:

12/2009

Belge Dili:

Türkçe

Document ID (Org No.):

2000755189

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Key words: Lung transplantation

organ preservation

Perfadex

methylprednisolone

rat

Sayı

Makale detayları

The effects of adding methylprednisolone to low-potassium dextran solution on preserved ischemic lungs

Yazarlar: Ali YEGİNSU | Makbule ERGİN | Hüseyin ÖZYURT | Çiğdem ELMAS | Güleser ÇAĞLAR GÖKTAŞ

Yayıncı: TUBITAK

Dergi: Turkish Journal of Medical Sciences 2010 Vol.40 Issue 1, pp.39-46

Belge Tipi: Makale

Yayın Tarihi: 12/2009

Belge Dili: Türkçe

Document ID (Org No.): 2000755189

Sıklık: İki Aylık

eISSN: 1303-6165

pISSN: 1300-0144

Key words: Lung transplantation

organ preservation

Perfadex

methylprednisolone

rat

Sayı

Yazarlar:

Ali YEGİNSU | Makbule ERGİN | Hüseyin ÖZYURT | Çiğdem ELMAS | Güleser ÇAĞLAR GÖKTAŞ

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2010 Vol.40 Issue 1, pp.39-46

Belge Tipi:

Makale

Yayın Tarihi:

12/2009

Belge Dili:

Türkçe

Document ID (Org No.):

2000755189

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144