Determination of the Antimicrobial and Biochemical Content of Black Carrot (Daucus carota ssp. sativus var. atrorubens Alef.)

Gizem Gül; Cenker Yaman; Dilay Turu; Atakan Benek; Rüveyde Pala; Merve Ülkü Sayım; Mustafa Eray Bozyel; Kerem Canlı

Makale detayları

Determination of the Antimicrobial and Biochemical Content of Black Carrot (Daucus carota ssp. sativus var. atrorubens Alef.)

Yazarlar:

Gizem Gül | Cenker Yaman | Dilay Turu | Atakan Benek | Rüveyde Pala | Merve Ülkü Sayım | Mustafa Eray Bozyel | Kerem Canlı

Yayıncı:

Afyon Kocatepe University

Ana Başlıklar:

Ekoloji (Diğer)

Dergi:

Afyon Kocatepe Üniversitesi Fen Ve Mühendislik Bilimleri Dergisi Vol.25 Issue 2, pp.253-260

Belge Tipi:

Makale

Yayın Tarihi:

11/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001344400

pISSN:

2149-3367

Anahtar kelimeler:

Özet:

 This study aimed to assess the antimicrobial activity of Daucus carota ssp. sativus var. atrorubens Alef. plant through disk diffusion and MIC Minimum Inhibitory Concentration methods, evaluate its antioxidant activity via the DPPH 2,2-diphenyl-1-picrylhydrazyl method, and analyze its biochemical composition using GC-MS Gas Chromatography-Mass Spectrometry. A total of 28 microorganisms were utilized, comprising 10 standard isolates, 1 clinical isolate, 7 food isolates, 9 multidrug-resistant strains, and 1 standard yeast. The most significant inhibition zones in the disk diffusion test were observed with measurements of 8-8-10 mm for the Staphylococcus aureus strain, 9-11-16 mm for the Enterococcus faecium strain, and 9-9-9 mm for the Streptococcus pneumonia strain. The MIC test revealed the highest inhibition at a concentration of 1.18 mgml against Enterococcus faecium strain. Antioxidant activity, determined using the DPPH method, exhibited the highest activity at a concentration of 1000 µgmL. GC-MS analysis identified Linoelaidic acid 18.30 as the predominant compound. Overall, the study detected antimicrobial activity against 21 out of the 28 tested microorganisms, highlighting the potent antimicrobial and antioxidant properties of D. carota ssp. sativus var. atrorubens, alongside its rich biochemical composition.

 This study aimed to assess the antimicrobial activity of Daucus carota ssp. sativus var. atrorubens Alef. plant through disk diffusion and MIC Minimum Inhibitory Concentration methods, evaluate its antioxidant activity via the DPPH 2,2-diphenyl-1-picrylhydrazyl method, and analyze its biochemical composition using GC-MS Gas Chromatography-Mass Spectrometry. A total of 28 microorganisms were utilized, comprising 10 standard isolates, 1 clinical isolate, 7 food isolates, 9 multidrug-resistant strains, and 1 standard yeast. The most significant inhibition zones in the disk diffusion test were observed with measurements of 8-8-10 mm for the Staphylococcus aureus strain, 9-11-16 mm for the Enterococcus faecium strain, and 9-9-9 mm for the Streptococcus pneumonia strain. The MIC test revealed the highest inhibition at a concentration of 1.18 mgml against Enterococcus faecium strain. Antioxidant activity, determined using the DPPH method, exhibited the highest activity at a concentration of 1000 µgmL. GC-MS analysis identified Linoelaidic acid 18.30 as the predominant compound. Overall, the study detected antimicrobial activity against 21 out of the 28 tested microorganisms, highlighting the potent antimicrobial and antioxidant properties of D. carota ssp. sativus var. atrorubens, alongside its rich biochemical composition.

 This study aimed to assess the antimicrobial activity of Daucus carota ssp. sativus var. atrorubens Alef. plant through disk diffusion and MIC Minimum Inhibitory Concentration methods, evaluate its antioxidant activity via the DPPH 2,2-diphenyl-1-picrylhydrazyl method, and analyze its biochemical composition using GC-MS Gas Chromatography-Mass Spectrometry. A total of 28 microorganisms were utilized, comprising 10 standard isolates, 1 clinical isolate, 7 food isolates, 9 multidrug-resistant strains, and 1 standard yeast. The most significant inhibition zones in the disk diffusion test were observed with measurements of 8-8-10 mm for the Staphylococcus aureus strain, 9-11-16 mm for the Enterococcus faecium strain, and 9-9-9 mm for the Streptococcus pneumonia strain. The MIC test revealed the highest inhibition at a concentration of 1.18 mgml against Enterococcus faecium strain. Antioxidant activity, determined using the DPPH method, exhibited the highest activity at a concentration of 1000 µgmL. GC-MS analysis identified Linoelaidic acid 18.30 as the predominant compound. Overall, the study detected antimicrobial activity against 21 out of the 28 tested microorganisms, highlighting the potent antimicrobial and antioxidant properties of D. carota ssp. sativus var. atrorubens, alongside its rich biochemical composition.

 Bu çalışmada, Daucus carota ssp. sativus var. atrorubens Alef. bitkisinin disk difüzyon ve MİK yöntemi ile antimikrobiyal aktivitesinin, DPPH yöntemi ile antioksidan aktivitesinin ve GC-MS yöntemi ile biyokimyasal içeriğinin belirlenmesi amaçlanmıştır. 10 standart izole, 1 klinik izole, 7 gıda izole, 9 çoklu ilaca dirençli suş ve 1 standart maya olmak üzere toplamda 28 mikroorganizma kullanılmıştır. Disk difüzyon testinde en yüksek sonuçlar Staphylococcus aureus suşunda 8-8-10 mm, Enterococcus faecium suşunda 9-11-16 mm ve Streptococcus pneumonia suşunda 9-9-9 mm zonlar gözlemlenmiştir. MİC testinde en yüksek sonuç Enterococcus faecium suşunda 1,18 mgml olarak tespit edilmiştir. Antioksidan aktivitesi DPPH yöntemi ile belirlenmiştir ve 1000 µgmL konsantrasyonunda en yüksek antioksidan aktiviteyi göstermiştir. GC-MS analizinin sonucunda majör madde olarak Linoelaidik asit 18,30 tespit edilmiştir. Sonuç olarak 28 mikroorganizma ile yapılan bu testte 21 mikroorganizmaya karşı antimikrobiyal etkinlik saptanmıştır. D. carota ssp. sativus var. atrorubensin güçlü bir antimikrobiyal ve antioksidan aktiviteye sahip olduğu ve bununla birlikte zengin bir biyokimyasal içerik barındırdığı tespit edilmiştir.

Makale detayları

Determination of the Antimicrobial and Biochemical Content of Black Carrot (Daucus carota ssp. sativus var. atrorubens Alef.)

Yazarlar:

Gizem Gül | Cenker Yaman | Dilay Turu | Atakan Benek | Rüveyde Pala | Merve Ülkü Sayım | Mustafa Eray Bozyel | Kerem Canlı

Yayıncı:

Afyon Kocatepe University

Ana Başlıklar:

Ekoloji (Diğer)

Dergi:

Afyon Kocatepe Üniversitesi Fen Ve Mühendislik Bilimleri Dergisi Vol.25 Issue 2, pp.253-260

Belge Tipi:

Makale

Yayın Tarihi:

11/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001344400

pISSN:

2149-3367

Daucus carota ssp. sativus var. atrorubens Alef.

Antimicrobial activity

Antioxidant activity

DPPH

GC-MS

Linoelaidic acidDaucus carota ssp. sativus var. atrorubens Alef.

Antimikrobiyal aktivite

Antioksidan aktivite

DPPH

GC-MS

Linoelaidik asit

Sayı

Makale detayları

Yazarlar: Gizem Gül | Cenker Yaman | Dilay Turu | Atakan Benek | Rüveyde Pala | Merve Ülkü Sayım | Mustafa Eray Bozyel | Kerem Canlı

Yayıncı: Afyon Kocatepe University

Ana Başlıklar: Ekoloji (Diğer)

Dergi: Afyon Kocatepe Üniversitesi Fen Ve Mühendislik Bilimleri Dergisi Vol.25 Issue 2, pp.253-260

Belge Tipi: Makale

Yayın Tarihi: 11/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001344400

pISSN: 2149-3367

Sayı

Yazarlar:

Gizem Gül | Cenker Yaman | Dilay Turu | Atakan Benek | Rüveyde Pala | Merve Ülkü Sayım | Mustafa Eray Bozyel | Kerem Canlı

Yayıncı:

Afyon Kocatepe University

Ana Başlıklar:

Ekoloji (Diğer)

Dergi:

Afyon Kocatepe Üniversitesi Fen Ve Mühendislik Bilimleri Dergisi Vol.25 Issue 2, pp.253-260

Belge Tipi:

Makale

Yayın Tarihi:

11/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001344400

pISSN:

2149-3367