Assessment of Saccharomyces boulardii effect on rats Staphylococcus aureus induced skin infection: an in-vivo study

Zahraa Amer Hashim

Makale detayları

Assessment of Saccharomyces boulardii effect on rats Staphylococcus aureus induced skin infection: an in-vivo study

Yazarlar:

Zahraa Amer Hashim

Yayıncı:

Marmara University

Ana Başlıklar:

Clinical Pharmacy and Pharmacy Practice

Dergi:

Journal of Research in Pharmacy Vol.26 Issue 5, pp.1342-1351

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001347553

pISSN:

2630-6344

Anahtar kelimeler:

Özet:

 A concerning global health issue is the growing incidence of antibiotic resistance in skin and soft tissue infection. This study aimed to assess the healing potential of topically applied Saccharomyces boulardii on excisional skin wound infected with Staphylococcus aureus using a rat model. The wounded animals were divided into three groups; group 1: uninfected untreated, group 2: infected untreated and group 3: infected S. boulardii-treated. Treatment commenced 2 days post infection. Parameters studied were wound microbial load, rate of wound contraction, serum C-reactive protein CRP level and rat β-defensin 1 RBD-1 gene expression. Staphylococcal bacterial load decline was significantly p  0.05 higher in group 3 when compared to group 2 at day 5, 6 and 7 accompanied by a growing of S. boulardii load on the animals of group 3. A complete wound closure 100 was monitored in study group 3 at day 7 in comparison to the control groups, where partial healing was achieved 67.10 and 91.74 in group 1 and 2 respectively. No significant difference of CRP level among the three groups was encountered indicating no influence of S. boulardii on this protein. RBD-1 gene expression in group 3 was found higher than the two control groups p  0.05 and  0.01 in comparison to group 1 and group 2 respectively. Topical application of S. boulardii was shown able to enhance wound healing and competitively exclude the infecting pathogen. The increased antimicrobial peptide gene expression in S. boulardii treated group might contribute to its healing potential.

 A concerning global health issue is the growing incidence of antibiotic resistance in skin and soft tissue infection. This study aimed to assess the healing potential of topically applied Saccharomyces boulardii on excisional skin wound infected with Staphylococcus aureus using a rat model. The wounded animals were divided into three groups; group 1: uninfected untreated, group 2: infected untreated and group 3: infected S. boulardii-treated. Treatment commenced 2 days post infection. Parameters studied were wound microbial load, rate of wound contraction, serum C-reactive protein CRP level and rat β-defensin 1 RBD-1 gene expression. Staphylococcal bacterial load decline was significantly p  0.05 higher in group 3 when compared to group 2 at day 5, 6 and 7 accompanied by a growing of S. boulardii load on the animals of group 3. A complete wound closure 100 was monitored in study group 3 at day 7 in comparison to the control groups, where partial healing was achieved 67.10 and 91.74 in group 1 and 2 respectively. No significant difference of CRP level among the three groups was encountered indicating no influence of S. boulardii on this protein. RBD-1 gene expression in group 3 was found higher than the two control groups p  0.05 and  0.01 in comparison to group 1 and group 2 respectively. Topical application of S. boulardii was shown able to enhance wound healing and competitively exclude the infecting pathogen. The increased antimicrobial peptide gene expression in S. boulardii treated group might contribute to its healing potential.

 A concerning global health issue is the growing incidence of antibiotic resistance in skin and soft tissue infection. This study aimed to assess the healing potential of topically applied Saccharomyces boulardii on excisional skin wound infected with Staphylococcus aureus using a rat model. The wounded animals were divided into three groups; group 1: uninfected untreated, group 2: infected untreated and group 3: infected S. boulardii-treated. Treatment commenced 2 days post infection. Parameters studied were wound microbial load, rate of wound contraction, serum C-reactive protein CRP level and rat β-defensin 1 RBD-1 gene expression. Staphylococcal bacterial load decline was significantly p  0.05 higher in group 3 when compared to group 2 at day 5, 6 and 7 accompanied by a growing of S. boulardii load on the animals of group 3. A complete wound closure 100 was monitored in study group 3 at day 7 in comparison to the control groups, where partial healing was achieved 67.10 and 91.74 in group 1 and 2 respectively. No significant difference of CRP level among the three groups was encountered indicating no influence of S. boulardii on this protein. RBD-1 gene expression in group 3 was found higher than the two control groups p  0.05 and  0.01 in comparison to group 1 and group 2 respectively. Topical application of S. boulardii was shown able to enhance wound healing and competitively exclude the infecting pathogen. The increased antimicrobial peptide gene expression in S. boulardii treated group might contribute to its healing potential.

 A concerning global health issue is the growing incidence of antibiotic resistance in skin and soft tissue infection. This study aimed to assess the healing potential of topically applied Saccharomyces boulardii on excisional skin wound infected with Staphylococcus aureus using a rat model. The wounded animals were divided into three groups; group 1: uninfected untreated, group 2: infected untreated and group 3: infected S. boulardii-treated. Treatment commenced 2 days post infection. Parameters studied were wound microbial load, rate of wound contraction, serum C-reactive protein CRP level and rat β-defensin 1 RBD-1 gene expression. Staphylococcal bacterial load decline was significantly p  0.05 higher in group 3 when compared to group 2 at day 5, 6 and 7 accompanied by a growing of S. boulardii load on the animals of group 3. A complete wound closure 100 was monitored in study group 3 at day 7 in comparison to the control groups, where partial healing was achieved 67.10 and 91.74 in group 1 and 2 respectively. No significant difference of CRP level among the three groups was encountered indicating no influence of S. boulardii on this protein. RBD-1 gene expression in group 3 was found higher than the two control groups p  0.05 and  0.01 in comparison to group 1 and group 2 respectively. Topical application of S. boulardii was shown able to enhance wound healing and competitively exclude the infecting pathogen. The increased antimicrobial peptide gene expression in S. boulardii treated group might contribute to its healing potential.

Makale detayları

Assessment of Saccharomyces boulardii effect on rats Staphylococcus aureus induced skin infection: an in-vivo study

Yazarlar:

Zahraa Amer Hashim

Yayıncı:

Marmara University

Ana Başlıklar:

Clinical Pharmacy and Pharmacy Practice

Dergi:

Journal of Research in Pharmacy Vol.26 Issue 5, pp.1342-1351

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001347553

pISSN:

2630-6344

Bacterial load

C-reactive protein

defensin

gene expression

Saccharomyces boulardii

skin wound

Staphylococcus aureus

Sayı

Makale detayları

Yazarlar: Zahraa Amer Hashim

Yayıncı: Marmara University

Ana Başlıklar: Clinical Pharmacy and Pharmacy Practice

Dergi: Journal of Research in Pharmacy Vol.26 Issue 5, pp.1342-1351

Belge Tipi: Makale

Yayın Tarihi: 6/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001347553

pISSN: 2630-6344

Sayı

Yazarlar:

Zahraa Amer Hashim

Yayıncı:

Marmara University

Ana Başlıklar:

Clinical Pharmacy and Pharmacy Practice

Dergi:

Journal of Research in Pharmacy Vol.26 Issue 5, pp.1342-1351

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001347553

pISSN:

2630-6344