The choice of auxin analogue for in vitro root induction influences post-induction root development in Eucalyptus grandis

Muhammad NAKHOODA; Maria Paula WATT; David MYCOCK

Makale detayları

The choice of auxin analogue for in vitro root induction influences post-induction root development in Eucalyptus grandis

Yazarlar:

Muhammad NAKHOODA | Maria Paula WATT | David MYCOCK

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Agriculture and Forestry 2014 Vol.38 Issue 2, pp.258-266

Belge Tipi:

Makale

Yayın Tarihi:

5/2014

Belge Dili:

Türkçe

Document ID (Org No.):

2000807445

Sıklık:

İki Aylık

eISSN:

1303-6173

pISSN:

1300-011X

Anahtar kelimeler:

Özet:

 

Previous studies on in vitro rooting for improved micropropagation of eucalypts indicated that root graviperception and post-acclimatisation architecture are determined by the relative exogenous auxin analogue and its stability, supplied during the pre-rooting culture stages. The specific roles of indole-3-acetic acid (IAA) and indole-3-butyric acid (IBA) in the rooting medium on the in vitro root morphological processes were explored using a good-rooting clone. In vitro rooting percentage was significantly reduced when either of the auxin inhibitors 2,3,5-triiodobenzoic acid (TIBA) and ρ-chlorophenoxyisobutyric acid (PCIB) or the auxin antagonist kinetin was supplied at rooting, with or without exogenous auxin. For all treatments, at the time of root induction, shoots did not possess a vascular cambium, only procambial tissue, from where adventitious roots formed. However, when the inhibitors or the antagonist were supplied to the roots 3 days after root induction, they affected root growth and graviperception. Kinetin and PCIB significantly reduced the mean root diameter from 552.8 µm (control) to 129.2 µm and 278.6 µm, respectively, over 3 weeks. While the PCIB treatment resulted in a significant increase in delta root length over this period, the TIBA treatment significantly decreased delta root length and increased mean root diameter to 833.4 µm. Restricting IAA transport with TIBA further altered root vascular patterning and, as with PCIB, resulted in the collapse of the columella region. Nevertheless, only a disruption in IAA transport and subsequent auxin distribution by TIBA treatment resulted in altered root graviperception. The results suggest the necessary inclusion of IAA in eucalypt micropropagation protocols to ensure good quality roots.

 

Previous studies on in vitro rooting for improved micropropagation of eucalypts indicated that root graviperception and post-acclimatisation architecture are determined by the relative exogenous auxin analogue and its stability, supplied during the pre-rooting culture stages. The specific roles of indole-3-acetic acid (IAA) and indole-3-butyric acid (IBA) in the rooting medium on the in vitro root morphological processes were explored using a good-rooting clone. In vitro rooting percentage was significantly reduced when either of the auxin inhibitors 2,3,5-triiodobenzoic acid (TIBA) and ρ-chlorophenoxyisobutyric acid (PCIB) or the auxin antagonist kinetin was supplied at rooting, with or without exogenous auxin. For all treatments, at the time of root induction, shoots did not possess a vascular cambium, only procambial tissue, from where adventitious roots formed. However, when the inhibitors or the antagonist were supplied to the roots 3 days after root induction, they affected root growth and graviperception. Kinetin and PCIB significantly reduced the mean root diameter from 552.8 µm (control) to 129.2 µm and 278.6 µm, respectively, over 3 weeks. While the PCIB treatment resulted in a significant increase in delta root length over this period, the TIBA treatment significantly decreased delta root length and increased mean root diameter to 833.4 µm. Restricting IAA transport with TIBA further altered root vascular patterning and, as with PCIB, resulted in the collapse of the columella region. Nevertheless, only a disruption in IAA transport and subsequent auxin distribution by TIBA treatment resulted in altered root graviperception. The results suggest the necessary inclusion of IAA in eucalypt micropropagation protocols to ensure good quality roots.

Makale detayları

The choice of auxin analogue for in vitro root induction influences post-induction root development in Eucalyptus grandis

Yazarlar:

Muhammad NAKHOODA | Maria Paula WATT | David MYCOCK

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Agriculture and Forestry 2014 Vol.38 Issue 2, pp.258-266

Belge Tipi:

Makale

Yayın Tarihi:

5/2014

Belge Dili:

Türkçe

Document ID (Org No.):

2000807445

Sıklık:

İki Aylık

eISSN:

1303-6173

pISSN:

1300-011X

Key words: Eucalyptus

graviperception

root cap

root quality

Sayı

Makale detayları

The choice of auxin analogue for in vitro root induction influences post-induction root development in Eucalyptus grandis

Yazarlar: Muhammad NAKHOODA | Maria Paula WATT | David MYCOCK

Yayıncı: TUBITAK

Dergi: Turkish Journal of Agriculture and Forestry 2014 Vol.38 Issue 2, pp.258-266

Belge Tipi: Makale

Yayın Tarihi: 5/2014

Belge Dili: Türkçe

Document ID (Org No.): 2000807445

Sıklık: İki Aylık

eISSN: 1303-6173

pISSN: 1300-011X

Key words: Eucalyptus

graviperception

root cap

root quality

Sayı

Yazarlar:

Muhammad NAKHOODA | Maria Paula WATT | David MYCOCK

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Agriculture and Forestry 2014 Vol.38 Issue 2, pp.258-266

Belge Tipi:

Makale

Yayın Tarihi:

5/2014

Belge Dili:

Türkçe

Document ID (Org No.):

2000807445

Sıklık:

İki Aylık

eISSN:

1303-6173

pISSN:

1300-011X