Assessment of cfDNA Levels in Saliva Samples of Stressed Young Adults: Preliminary Study

Ekin Çelik

Makale detayları

Assessment of cfDNA Levels in Saliva Samples of Stressed Young Adults: Preliminary Study

Yazarlar:

Ekin Çelik

Yayıncı:

Kirsehir Ahi Evran University

Ana Başlıklar:

Tıbbi Genetik (Kanser Genetiği hariç)

Dergi:

Ahi Evran Tıp Dergisi Vol.9 Issue 1, pp.12-16

Belge Tipi:

Makale

Yayın Tarihi:

4/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001344990

pISSN:

2619-9203

Anahtar kelimeler:

Özet:

 Purpose: The aim of this pilot study was to examine the levels of cell-free DNA cfDNA in the saliva samples of second-year medical students before and after a stress-inducing event, exams. Materials and Methods: Saliva samples were collected from 20 students, whose stress profiles were assessed using the DASS-21 scale, both before and after the exams. Cell-free DNA was isolated from these samples, and measured using spectrophotometry and automated electrophoresis system. Results: Spectrophotometric analysis revealed a significant decrease in cfDNA levels in the saliva samples collected after the exams p0.05. This difference was confirmed by the automated electrophoresis system, particularly showing a reduction in cfDNA amounts in the 40-200 bp range with the reduction of stress p0.05. Conclusion: Psychosocial stress affects the release of cell-free DNA. This study reports that the amount of cfDNA found in saliva samples significantly changes in relation to stress. This preliminary study suggests that cfDNA in saliva samples could potentially serve as a biological marker for stress in healthy individuals.

 Purpose: The aim of this pilot study was to examine the levels of cell-free DNA cfDNA in the saliva samples of second-year medical students before and after a stress-inducing event, exams. Materials and Methods: Saliva samples were collected from 20 students, whose stress profiles were assessed using the DASS-21 scale, both before and after the exams. Cell-free DNA was isolated from these samples, and measured using spectrophotometry and automated electrophoresis system. Results: Spectrophotometric analysis revealed a significant decrease in cfDNA levels in the saliva samples collected after the exams p0.05. This difference was confirmed by the automated electrophoresis system, particularly showing a reduction in cfDNA amounts in the 40-200 bp range with the reduction of stress p0.05. Conclusion: Psychosocial stress affects the release of cell-free DNA. This study reports that the amount of cfDNA found in saliva samples significantly changes in relation to stress. This preliminary study suggests that cfDNA in saliva samples could potentially serve as a biological marker for stress in healthy individuals.

 Purpose: The aim of this pilot study was to examine the levels of cell-free DNA cfDNA in the saliva samples of second-year medical students before and after a stress-inducing event, exams. Materials and Methods: Saliva samples were collected from 20 students, whose stress profiles were assessed using the DASS-21 scale, both before and after the exams. Cell-free DNA was isolated from these samples, and measured using spectrophotometry and automated electrophoresis system. Results: Spectrophotometric analysis revealed a significant decrease in cfDNA levels in the saliva samples collected after the exams p0.05. This difference was confirmed by the automated electrophoresis system, particularly showing a reduction in cfDNA amounts in the 40-200 bp range with the reduction of stress p0.05. Conclusion: Psychosocial stress affects the release of cell-free DNA. This study reports that the amount of cfDNA found in saliva samples significantly changes in relation to stress. This preliminary study suggests that cfDNA in saliva samples could potentially serve as a biological marker for stress in healthy individuals.

 Amaç: Bu pilot çalışmada, ikinci sınıf tıp öğrencilerinin sınav öncesi ve sonrasında tükürük örneklerindeki hücre dışı DNA hdDNA, cfDNA düzeylerinin incelenmesi amaçlanmıştır. Araçlar ve Yöntem: Stres profilleri DASS-21 ölçeği ile değerlendirilen 20 öğrencinin sınav öncesi ve sonrası tükürük örnekleri alınarak hücre dışı DNA izole edilmiştir. İzole edilen DNAlar spektrofotometre ve otomatik elektroforez sistemi ile ölçülmüştür. Bulgular: Spektrofotometre analizleri, sınav sonrası öğrencilerden alınan cfDNA miktarlarında anlamlı bir azalma göstermiştir p0.05. Bu fark otomatik elektroforez sistemi ile doğrulanmış ve özellikle 40-200 bp aralığındaki cfDNA miktarlarının stresin azalmasıyla birlikte azaldığı tespit edilmiştir p0.05. Sonuç: Psikososyal stres hdDNA salınımını etkilemektedir. Bu çalışmada, tükürük örneklerinde bulunan hdDNA miktarlarının stresle ilişkili olarak anlamlı değişiklikler gösterdiği raporlanmıştır. Bu ön çalışma, tükürük numunelerindeki hdDNAnın sağlıklı bireylerde stresin potansiyel biyolojik bir belirteci olarak değerlendirilebileceğini ortaya koymuştur.

Makale detayları

Assessment of cfDNA Levels in Saliva Samples of Stressed Young Adults: Preliminary Study

Yazarlar:

Ekin Çelik

Yayıncı:

Kirsehir Ahi Evran University

Ana Başlıklar:

Tıbbi Genetik (Kanser Genetiği hariç)

Dergi:

Ahi Evran Tıp Dergisi Vol.9 Issue 1, pp.12-16

Belge Tipi:

Makale

Yayın Tarihi:

4/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001344990

pISSN:

2619-9203

biomarker

cfDNA

plasmabiyobelirteç

hdDNA

plazma

Sayı

Makale detayları

Assessment of cfDNA Levels in Saliva Samples of Stressed Young Adults: Preliminary Study

Yazarlar: Ekin Çelik

Yayıncı: Kirsehir Ahi Evran University

Ana Başlıklar: Tıbbi Genetik (Kanser Genetiği hariç)

Dergi: Ahi Evran Tıp Dergisi Vol.9 Issue 1, pp.12-16

Belge Tipi: Makale

Yayın Tarihi: 4/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001344990

pISSN: 2619-9203

biomarker

cfDNA

plasmabiyobelirteç

hdDNA

plazma

Sayı

Yazarlar:

Ekin Çelik

Yayıncı:

Kirsehir Ahi Evran University

Ana Başlıklar:

Tıbbi Genetik (Kanser Genetiği hariç)

Dergi:

Ahi Evran Tıp Dergisi Vol.9 Issue 1, pp.12-16

Belge Tipi:

Makale

Yayın Tarihi:

4/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001344990

pISSN:

2619-9203