Detection of the cry1 gene in Bacillus thuringiensis isolates from agricultural fields and their bioactivity against two stored product moth larvae

İlyas BOZLAĞAN; Abdurrahman AYVAZ; Fatma ÖZTÜRK; Leyla AÇIK

Makale detayları

Detection of the cry1 gene in Bacillus thuringiensis isolates from agricultural fields and their bioactivity against two stored product moth larvae

Yazarlar:

İlyas BOZLAĞAN | Abdurrahman AYVAZ | Fatma ÖZTÜRK | Leyla AÇIK

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Agriculture and Forestry 2010 Vol.34 Issue 2, pp.145-154

Belge Tipi:

Makale

Yayın Tarihi:

1/2010

Belge Dili:

Türkçe

Document ID (Org No.):

2000808327

Sıklık:

İki Aylık

eISSN:

1303-6173

pISSN:

1300-011X

Anahtar kelimeler:

Özet:

 

Sixty Bacillus spp. isolates were obtained from different agricultural fields in Kayseri province using selective media. Total DNA was isolated and analyzed by PCR using cry1 general primers. Of the 60 tested isolates, 17 carried the cry1 gene. B. thuringiensis subsp. kurstaki and all other isolates exhibited approximately 19-kb plasmid bands.  In addition to the 270-bp band, the standard strain and isolate I47 yielded 180-bp PCR products. The isolates and standard strain were toxic to Ephestia kuehniella Zeller and Plodia interpunctella Hubner larvae. Insecticidal activity of isolates I36, I37, and I40 against Ephestia kuehniella was higher than that of the standard strain when 106 spore-crystals mL-1 was used. LC99 values for isolates I36, I37, and I40 were 6.46, 6.28, and 6.24 for E. kuehniella, respectively, and these isolates were also effective against Plodia interpunctella larvae. LC99 values for isolates I36 and I40 were 6.69 and 5.07 for P. interpunctella, respectively. The results clearly show that isolates I36, I37, and I40 were effective at lower doses, as compared to the others.

 

Sixty Bacillus spp. isolates were obtained from different agricultural fields in Kayseri province using selective media. Total DNA was isolated and analyzed by PCR using cry1 general primers. Of the 60 tested isolates, 17 carried the cry1 gene. B. thuringiensis subsp. kurstaki and all other isolates exhibited approximately 19-kb plasmid bands.  In addition to the 270-bp band, the standard strain and isolate I47 yielded 180-bp PCR products. The isolates and standard strain were toxic to Ephestia kuehniella Zeller and Plodia interpunctella Hubner larvae. Insecticidal activity of isolates I36, I37, and I40 against Ephestia kuehniella was higher than that of the standard strain when 106 spore-crystals mL-1 was used. LC99 values for isolates I36, I37, and I40 were 6.46, 6.28, and 6.24 for E. kuehniella, respectively, and these isolates were also effective against Plodia interpunctella larvae. LC99 values for isolates I36 and I40 were 6.69 and 5.07 for P. interpunctella, respectively. The results clearly show that isolates I36, I37, and I40 were effective at lower doses, as compared to the others.

Makale detayları

Detection of the cry1 gene in Bacillus thuringiensis isolates from agricultural fields and their bioactivity against two stored product moth larvae

Yazarlar:

İlyas BOZLAĞAN | Abdurrahman AYVAZ | Fatma ÖZTÜRK | Leyla AÇIK

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Agriculture and Forestry 2010 Vol.34 Issue 2, pp.145-154

Belge Tipi:

Makale

Yayın Tarihi:

1/2010

Belge Dili:

Türkçe

Document ID (Org No.):

2000808327

Sıklık:

İki Aylık

eISSN:

1303-6173

pISSN:

1300-011X

Key words: Bacillus thuringiensis

cry1 primer

Ephestia kuehniella

Plodia interpunctella

Sayı

Makale detayları

Detection of the cry1 gene in Bacillus thuringiensis isolates from agricultural fields and their bioactivity against two stored product moth larvae

Yazarlar: İlyas BOZLAĞAN | Abdurrahman AYVAZ | Fatma ÖZTÜRK | Leyla AÇIK

Yayıncı: TUBITAK

Dergi: Turkish Journal of Agriculture and Forestry 2010 Vol.34 Issue 2, pp.145-154

Belge Tipi: Makale

Yayın Tarihi: 1/2010

Belge Dili: Türkçe

Document ID (Org No.): 2000808327

Sıklık: İki Aylık

eISSN: 1303-6173

pISSN: 1300-011X

Key words: Bacillus thuringiensis

cry1 primer

Ephestia kuehniella

Plodia interpunctella

Sayı

Yazarlar:

İlyas BOZLAĞAN | Abdurrahman AYVAZ | Fatma ÖZTÜRK | Leyla AÇIK

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Agriculture and Forestry 2010 Vol.34 Issue 2, pp.145-154

Belge Tipi:

Makale

Yayın Tarihi:

1/2010

Belge Dili:

Türkçe

Document ID (Org No.):

2000808327

Sıklık:

İki Aylık

eISSN:

1303-6173

pISSN:

1300-011X