Development and Validation of Liquid Chromatography/Stable Isotope-Dilution Tandem Mass Spectrometry Method for Measurement of Dexamethasone, Total Cortisol and Free Cortisol in Human Plasma

Gamze Tuna; Melis Kant; Merve Akış; Serkan Yener; Gül Hüray İşlekel

Makale detayları

Development and Validation of Liquid Chromatography/Stable Isotope-Dilution Tandem Mass Spectrometry Method for Measurement of Dexamethasone, Total Cortisol and Free Cortisol in Human Plasma

Yazarlar:

Gamze Tuna | Melis Kant | Merve Akış | Serkan Yener | Gül Hüray İşlekel

Yayıncı:

Dokuz Eylul University

Ana Başlıklar:

Health Care Administration

Dergi:

Journal of Basic and Clinical Health Sciences Vol.7 Issue 1, pp.516-524

Belge Tipi:

Makale

Yayın Tarihi:

1/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001283443

pISSN:

2564-7288

Anahtar kelimeler:

Özet:

 Background Cortisol plays a role in the regulation of metabolic homeostasis. Dexamethasone is a powerful synthetic glucocorticoid that acts as an anti-inflammatory and immunosuppressive agent. Plasma free cortisol, which has a low concentration in the blood, is the cortisol fraction that provides biological activity. It is emphasized that measurement of dexamethasone, total and free cortisol levels can directly affect the results of dexamethasone suppression test, which is routinely used to exclude endogenous hypercortisolemia in patients with adrenal adenoma, and is important in differential diagnosis. Purpose In this study, a precise and rapid method was developed for the measurement of dexamethasone, total and free cortisol levels in plasma samples, besides demonstrating the clinical applicability of the method with samples from adrenal adenoma patients. Methods Method optimization studies were performed using liquid chromatography-tandem mass spectrometry with stable isotope dilution-multiple reaction monitoring. Results The assay demonstrated a good linear dynamic range of 0.520 μgL, 5200 μgL and 0.5100 μgL for dexamethasone, total and free cortisol, respectively. The values for intra- and inter-day precisions of analytes were 6.9 with the accuracies ranging from 91.6 to 113.0. Conclusion Measurement of these parameters can be used reliably to diagnose diseases causing hypercortisolemia.

 Background Cortisol plays a role in the regulation of metabolic homeostasis. Dexamethasone is a powerful synthetic glucocorticoid that acts as an anti-inflammatory and immunosuppressive agent. Plasma free cortisol, which has a low concentration in the blood, is the cortisol fraction that provides biological activity. It is emphasized that measurement of dexamethasone, total and free cortisol levels can directly affect the results of dexamethasone suppression test, which is routinely used to exclude endogenous hypercortisolemia in patients with adrenal adenoma, and is important in differential diagnosis. Purpose In this study, a precise and rapid method was developed for the measurement of dexamethasone, total and free cortisol levels in plasma samples, besides demonstrating the clinical applicability of the method with samples from adrenal adenoma patients. Methods Method optimization studies were performed using liquid chromatography-tandem mass spectrometry with stable isotope dilution-multiple reaction monitoring. Results The assay demonstrated a good linear dynamic range of 0.520 μgL, 5200 μgL and 0.5100 μgL for dexamethasone, total and free cortisol, respectively. The values for intra- and inter-day precisions of analytes were 6.9 with the accuracies ranging from 91.6 to 113.0. Conclusion Measurement of these parameters can be used reliably to diagnose diseases causing hypercortisolemia.

 Background Cortisol plays a role in the regulation of metabolic homeostasis. Dexamethasone is a powerful synthetic glucocorticoid that acts as an anti-inflammatory and immunosuppressive agent. Plasma free cortisol, which has a low concentration in the blood, is the cortisol fraction that provides biological activity. It is emphasized that measurement of dexamethasone, total and free cortisol levels can directly affect the results of dexamethasone suppression test, which is routinely used to exclude endogenous hypercortisolemia in patients with adrenal adenoma, and is important in differential diagnosis. Purpose In this study, a precise and rapid method was developed for the measurement of dexamethasone, total and free cortisol levels in plasma samples, besides demonstrating the clinical applicability of the method with samples from adrenal adenoma patients. Methods Method optimization studies were performed using liquid chromatography-tandem mass spectrometry with stable isotope dilution-multiple reaction monitoring. Results The assay demonstrated a good linear dynamic range of 0.520 μgL, 5200 μgL and 0.5100 μgL for dexamethasone, total and free cortisol, respectively. The values for intra- and inter-day precisions of analytes were 6.9 with the accuracies ranging from 91.6 to 113.0. Conclusion Measurement of these parameters can be used reliably to diagnose diseases causing hypercortisolemia.

 Background Cortisol plays a role in the regulation of metabolic homeostasis. Dexamethasone is a powerful synthetic glucocorticoid that acts as an anti-inflammatory and immunosuppressive agent. Plasma free cortisol, which has a low concentration in the blood, is the cortisol fraction that provides biological activity. It is emphasized that measurement of dexamethasone, total and free cortisol levels can directly affect the results of dexamethasone suppression test, which is routinely used to exclude endogenous hypercortisolemia in patients with adrenal adenoma, and is important in differential diagnosis. Purpose In this study, a precise and rapid method was developed for the measurement of dexamethasone, total and free cortisol levels in plasma samples, besides demonstrating the clinical applicability of the method with samples from adrenal adenoma patients. Methods Method optimization studies were performed using liquid chromatography-tandem mass spectrometry with stable isotope dilution-multiple reaction monitoring. Results The assay demonstrated a good linear dynamic range of 0.520 μgL, 5200 μgL and 0.5100 μgL for dexamethasone, total and free cortisol, respectively. The values for intra- and inter-day precisions of analytes were 6.9 with the accuracies ranging from 91.6 to 113.0. Conclusion Measurement of these parameters can be used reliably to diagnose diseases causing hypercortisolemia.

Makale detayları

Development and Validation of Liquid Chromatography/Stable Isotope-Dilution Tandem Mass Spectrometry Method for Measurement of Dexamethasone, Total Cortisol and Free Cortisol in Human Plasma

Yazarlar:

Gamze Tuna | Melis Kant | Merve Akış | Serkan Yener | Gül Hüray İşlekel

Yayıncı:

Dokuz Eylul University

Ana Başlıklar:

Health Care Administration

Dergi:

Journal of Basic and Clinical Health Sciences Vol.7 Issue 1, pp.516-524

Belge Tipi:

Makale

Yayın Tarihi:

1/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001283443

pISSN:

2564-7288

Adrenal adenoma

dexamethasone

free cortisol

LC-MS/MS

method validation

total cortisol

Adrenal adenoma

dexamethasone

free cortisol

LC-MS/MS

method validation

total cortisol

Sayı

Makale detayları

Yazarlar: Gamze Tuna | Melis Kant | Merve Akış | Serkan Yener | Gül Hüray İşlekel

Yayıncı: Dokuz Eylul University

Ana Başlıklar: Health Care Administration

Dergi: Journal of Basic and Clinical Health Sciences Vol.7 Issue 1, pp.516-524

Belge Tipi: Makale

Yayın Tarihi: 1/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001283443

pISSN: 2564-7288

Sayı

Yazarlar:

Gamze Tuna | Melis Kant | Merve Akış | Serkan Yener | Gül Hüray İşlekel

Yayıncı:

Dokuz Eylul University

Ana Başlıklar:

Health Care Administration

Dergi:

Journal of Basic and Clinical Health Sciences Vol.7 Issue 1, pp.516-524

Belge Tipi:

Makale

Yayın Tarihi:

1/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001283443

pISSN:

2564-7288