DNA barcoding and phylogenetic analysis of two species populations of Diplodus from the eastern Mediterranean coasts of Turkey

Makbule Baylan; Gamze Mazı; B. Devrim Özcan; Sedat Gundogdu; Dilek Tekdal

Makale detayları

DNA barcoding and phylogenetic analysis of two species populations of Diplodus from the eastern Mediterranean coasts of Turkey

Yazarlar:

Makbule Baylan | Gamze Mazı | B. Devrim Özcan | Sedat Gundogdu | Dilek Tekdal

Yayıncı:

Osmaniye Korkut Ata Üniversitesi

Ana Başlıklar:

Hayvansal Üretim (Diğer)

Dergi:

Osmaniye Korkut Ata Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.6 Issue 1, pp.806-817

Belge Tipi:

Makale

Yayın Tarihi:

10/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001284878

pISSN:

2687-3729

Anahtar kelimeler:

Özet:

 Since Turkey is surrounded by seas on three sides and has different climatic characteristics, it has an extremely rich biodiversity. Identifying fish species and varieties is of great importance for protecting them from drought conditions seen in recent years and using them as a source of human food protein. The purpose of this study was to identify the genetic diversity of Diplodus, one of the fish species the importance of which is under appreciated, in Turkish seas. To this end, the polymorphism between the populations of two species Diplodus vulgaris and Diplodus sargus from the Sparidae Coral fish family was determined using the nucleotide sequence of the mitochondrial cytochrome c oxidase I COI gene, which is 652 base pairs long. 143 specimens of Diplodus were collected from the locations in the Eastern Mediterranean İskenderun and Mersin and Western Mediterranean Antalya. The COI gene was amplified by PCR, genetic diversity analyses of these loci were carried out, and a phylogenetic tree was created to show the relationships between the isolates with a high difference. In conclusion, based on the haplotype and nucleotide diversity patterns, it was found that the COI gene had a low genetic variation among the populations.

 Since Turkey is surrounded by seas on three sides and has different climatic characteristics, it has an extremely rich biodiversity. Identifying fish species and varieties is of great importance for protecting them from drought conditions seen in recent years and using them as a source of human food protein. The purpose of this study was to identify the genetic diversity of Diplodus, one of the fish species the importance of which is under appreciated, in Turkish seas. To this end, the polymorphism between the populations of two species Diplodus vulgaris and Diplodus sargus from the Sparidae Coral fish family was determined using the nucleotide sequence of the mitochondrial cytochrome c oxidase I COI gene, which is 652 base pairs long. 143 specimens of Diplodus were collected from the locations in the Eastern Mediterranean İskenderun and Mersin and Western Mediterranean Antalya. The COI gene was amplified by PCR, genetic diversity analyses of these loci were carried out, and a phylogenetic tree was created to show the relationships between the isolates with a high difference. In conclusion, based on the haplotype and nucleotide diversity patterns, it was found that the COI gene had a low genetic variation among the populations.

 Since Turkey is surrounded by seas on three sides and has different climatic characteristics, it has an extremely rich biodiversity. Identifying fish species and varieties is of great importance for protecting them from drought conditions seen in recent years and using them as a source of human food protein. The purpose of this study was to identify the genetic diversity of Diplodus, one of the fish species the importance of which is under appreciated, in Turkish seas. To this end, the polymorphism between the populations of two species Diplodus vulgaris and Diplodus sargus from the Sparidae Coral fish family was determined using the nucleotide sequence of the mitochondrial cytochrome c oxidase I COI gene, which is 652 base pairs long. 143 specimens of Diplodus were collected from the locations in the Eastern Mediterranean İskenderun and Mersin and Western Mediterranean Antalya. The COI gene was amplified by PCR, genetic diversity analyses of these loci were carried out, and a phylogenetic tree was created to show the relationships between the isolates with a high difference. In conclusion, based on the haplotype and nucleotide diversity patterns, it was found that the COI gene had a low genetic variation among the populations.

 Türkiye üç tarafı denizlerle çevrili ve farklı iklimsel özelliklere sahip olduğundan biyoçeşitlilik açısından son derece zengin bir ülke konumundadır. Deniz ekosistemi için büyük öneme sahip balık tür ve çeşitlerinin tespiti gerek son yıllarda yadsınamaz derecede hissedilen kuraklık koşulları nedeni ile bu türlerin korunması ve gerekse de insan besin proteini açısından bu türlerin kullanılması bakımından önemlidir. Sunulan bu çalışmada, balık türleri içerisinde önemi en az bilinen türlerden olan Diplodus türlerinin Türkiye denizlerindeki genetik çeşitliliğinin belirlenmesi amaçlanmıştır. Bu amaçla, Sparidae Mercan balıkları familyasına ait iki türün Diplodus vulgaris ve Diplodus sargus populasyonları arasındaki polimorfizm, 652 baz çiftine sahip mitokondriyal sitokrom c oksidaz I COI geninin nükleotid dizisi kullanılarak belirlenmiştir. 143 Diplodus örneği Doğu Akdeniz İskenderun ve Mersin ve Batı Akdeniz Antalya lokalitelerinden toplanmıştır. Balık örneklerinden kas dokusu alınarak DNA izolasyonu yapılmıştır. PCR ile COI geni çoğaltılarak bu lokuslara ait genetik farklılık analizleri yapılmış ve farklılığın yüksek olduğu izolatlarda ilişkileri göstermek için filogenetik ağaç oluşturulmuştur. Sonuç olarak, COI geninin haplotip ve nükleotit çeşitliklerine dayalı olarak populasyonlar arasında düşük bir genetik varyasyona sahip olduğu saptanmıştır.

Makale detayları

DNA barcoding and phylogenetic analysis of two species populations of Diplodus from the eastern Mediterranean coasts of Turkey

Yazarlar:

Makbule Baylan | Gamze Mazı | B. Devrim Özcan | Sedat Gundogdu | Dilek Tekdal

Yayıncı:

Osmaniye Korkut Ata Üniversitesi

Ana Başlıklar:

Hayvansal Üretim (Diğer)

Dergi:

Osmaniye Korkut Ata Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.6 Issue 1, pp.806-817

Belge Tipi:

Makale

Yayın Tarihi:

10/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001284878

pISSN:

2687-3729

Diplodus vulgaris

Diplodus sargus

COI gene

Polymorphism

Phylogenetics

Diplodus vulgaris

Diplodus sargus

COI geni

polimorfizm

filogenetik

Sayı

Makale detayları

Yazarlar: Makbule Baylan | Gamze Mazı | B. Devrim Özcan | Sedat Gundogdu | Dilek Tekdal

Yayıncı: Osmaniye Korkut Ata Üniversitesi

Ana Başlıklar: Hayvansal Üretim (Diğer)

Dergi: Osmaniye Korkut Ata Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.6 Issue 1, pp.806-817

Belge Tipi: Makale

Yayın Tarihi: 10/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001284878

pISSN: 2687-3729

Sayı

Yazarlar:

Makbule Baylan | Gamze Mazı | B. Devrim Özcan | Sedat Gundogdu | Dilek Tekdal

Yayıncı:

Osmaniye Korkut Ata Üniversitesi

Ana Başlıklar:

Hayvansal Üretim (Diğer)

Dergi:

Osmaniye Korkut Ata Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.6 Issue 1, pp.806-817

Belge Tipi:

Makale

Yayın Tarihi:

10/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001284878

pISSN:

2687-3729