Effect of methylglyoxal on Parkinson’s disease pathophysiology in the rotenone model

Yekta Çulpan; Yakup Gozderesi; Betul Yılmaz; Rezzan Gülhan

Makale detayları

Effect of methylglyoxal on Parkinson’s disease pathophysiology in the rotenone model

Yazarlar:

Yekta Çulpan | Yakup Gozderesi | Betul Yılmaz | Rezzan Gülhan

Yayıncı:

Marmara University

Ana Başlıklar:

Surgery (Other)

Dergi:

Marmara Medical Journal Vol.37 Issue 2, pp.166-177

Belge Tipi:

Makale

Yayın Tarihi:

5/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001317363

pISSN:

1019-1941

Anahtar kelimeler:

Özet:

 Objective: Type 2 diabetes mellitus patients have been reported to have a higher incidence of Parkinsons disease. This study aimed to explore the effect of advanced glycation end products precursor methylglyoxal MGO on the pathophysiology of Parkinsons disease in a rotenone model. Materials and Methods: Adult female Wistar rats n42 were divided into four groups. Rotenone toxicity was assessed by daily weight measurements and mortality rates. Effect of MGO on blood glucose was evaluated. Locomotor activity, rearing, and rotarod tests were performed to evaluate motor functions, and for neurodegeneration, tyrosine hydroxylase immunoreactivity in the striatum and substantia nigra regions was assessed. Results: The mortality rate was 9 in the rotenone-applied rats. The mean weight, locomotor activity, rearing activity, and longest time spent on a rotarod were lower in the MGORotenone group than in the Control group. Tyrosine hydroxylase immunoreactivity in the striatum rostral to the anterior commissure in the MGORotenone group was lower than that in the Control and MGO groups. The number of tyrosine hydroxylase positive cells in the substantia nigra pars compacta was comparable among the groups. Conclusion: When nigrostriatal degeneration was triggered, MGO was found to worsen motor dysfunction and increase damage to dopaminergic neuron projections.

 Objective: Type 2 diabetes mellitus patients have been reported to have a higher incidence of Parkinsons disease. This study aimed to explore the effect of advanced glycation end products precursor methylglyoxal MGO on the pathophysiology of Parkinsons disease in a rotenone model. Materials and Methods: Adult female Wistar rats n42 were divided into four groups. Rotenone toxicity was assessed by daily weight measurements and mortality rates. Effect of MGO on blood glucose was evaluated. Locomotor activity, rearing, and rotarod tests were performed to evaluate motor functions, and for neurodegeneration, tyrosine hydroxylase immunoreactivity in the striatum and substantia nigra regions was assessed. Results: The mortality rate was 9 in the rotenone-applied rats. The mean weight, locomotor activity, rearing activity, and longest time spent on a rotarod were lower in the MGORotenone group than in the Control group. Tyrosine hydroxylase immunoreactivity in the striatum rostral to the anterior commissure in the MGORotenone group was lower than that in the Control and MGO groups. The number of tyrosine hydroxylase positive cells in the substantia nigra pars compacta was comparable among the groups. Conclusion: When nigrostriatal degeneration was triggered, MGO was found to worsen motor dysfunction and increase damage to dopaminergic neuron projections.

 Objective: Type 2 diabetes mellitus patients have been reported to have a higher incidence of Parkinsons disease. This study aimed to explore the effect of advanced glycation end products precursor methylglyoxal MGO on the pathophysiology of Parkinsons disease in a rotenone model. Materials and Methods: Adult female Wistar rats n42 were divided into four groups. Rotenone toxicity was assessed by daily weight measurements and mortality rates. Effect of MGO on blood glucose was evaluated. Locomotor activity, rearing, and rotarod tests were performed to evaluate motor functions, and for neurodegeneration, tyrosine hydroxylase immunoreactivity in the striatum and substantia nigra regions was assessed. Results: The mortality rate was 9 in the rotenone-applied rats. The mean weight, locomotor activity, rearing activity, and longest time spent on a rotarod were lower in the MGORotenone group than in the Control group. Tyrosine hydroxylase immunoreactivity in the striatum rostral to the anterior commissure in the MGORotenone group was lower than that in the Control and MGO groups. The number of tyrosine hydroxylase positive cells in the substantia nigra pars compacta was comparable among the groups. Conclusion: When nigrostriatal degeneration was triggered, MGO was found to worsen motor dysfunction and increase damage to dopaminergic neuron projections.

 Objective: Type 2 diabetes mellitus patients have been reported to have a higher incidence of Parkinsons disease. This study aimed to explore the effect of advanced glycation end products precursor methylglyoxal MGO on the pathophysiology of Parkinsons disease in a rotenone model. Materials and Methods: Adult female Wistar rats n42 were divided into four groups. Rotenone toxicity was assessed by daily weight measurements and mortality rates. Effect of MGO on blood glucose was evaluated. Locomotor activity, rearing, and rotarod tests were performed to evaluate motor functions, and for neurodegeneration, tyrosine hydroxylase immunoreactivity in the striatum and substantia nigra regions was assessed. Results: The mortality rate was 9 in the rotenone-applied rats. The mean weight, locomotor activity, rearing activity, and longest time spent on a rotarod were lower in the MGORotenone group than in the Control group. Tyrosine hydroxylase immunoreactivity in the striatum rostral to the anterior commissure in the MGORotenone group was lower than that in the Control and MGO groups. The number of tyrosine hydroxylase positive cells in the substantia nigra pars compacta was comparable among the groups. Conclusion: When nigrostriatal degeneration was triggered, MGO was found to worsen motor dysfunction and increase damage to dopaminergic neuron projections.

Makale detayları

Effect of methylglyoxal on Parkinson’s disease pathophysiology in the rotenone model

Yazarlar:

Yekta Çulpan | Yakup Gozderesi | Betul Yılmaz | Rezzan Gülhan

Yayıncı:

Marmara University

Ana Başlıklar:

Surgery (Other)

Dergi:

Marmara Medical Journal Vol.37 Issue 2, pp.166-177

Belge Tipi:

Makale

Yayın Tarihi:

5/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001317363

pISSN:

1019-1941

Parkinson’s disease

Type 2 diabetes mellitus

Methylglyoxal

Rotenone

Tyrosine hydroxylase

Locomotor activity

Sayı

Makale detayları

Effect of methylglyoxal on Parkinson’s disease pathophysiology in the rotenone model

Yazarlar: Yekta Çulpan | Yakup Gozderesi | Betul Yılmaz | Rezzan Gülhan

Yayıncı: Marmara University

Ana Başlıklar: Surgery (Other)

Dergi: Marmara Medical Journal Vol.37 Issue 2, pp.166-177

Belge Tipi: Makale

Yayın Tarihi: 5/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001317363

pISSN: 1019-1941

Parkinson’s disease

Type 2 diabetes mellitus

Methylglyoxal

Rotenone

Tyrosine hydroxylase

Locomotor activity

Sayı

Yazarlar:

Yekta Çulpan | Yakup Gozderesi | Betul Yılmaz | Rezzan Gülhan

Yayıncı:

Marmara University

Ana Başlıklar:

Surgery (Other)

Dergi:

Marmara Medical Journal Vol.37 Issue 2, pp.166-177

Belge Tipi:

Makale

Yayın Tarihi:

5/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001317363

pISSN:

1019-1941