Investigation of The Efficacy of Sericin in Experimental Knee Osteoarthritis Model in Rats through the TGF-Beta/Smad Pathway

Koksal Gundogdu; Özgen Kılıç Erkek; Gülşah Gündoğdu; Dilek Sayın; Gülçin Abban Mete

Makale detayları

Investigation of The Efficacy of Sericin in Experimental Knee Osteoarthritis Model in Rats through the TGF-Beta/Smad Pathway

Yazarlar:

Koksal Gundogdu | Özgen Kılıç Erkek | Gülşah Gündoğdu | Dilek Sayın | Gülçin Abban Mete

Yayıncı:

Ataturk University

Ana Başlıklar:

Veteriner Anatomi ve Fizyoloji | Veteriner Cerrahi | Veteriner Bilimleri (Diğer)

Dergi:

Veterinary Sciences and Practices Vol.19 Issue 2, pp.85-93

Belge Tipi:

Makale

Yayın Tarihi:

8/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001325986

pISSN:

2822-3608

Anahtar kelimeler:

Özet:

 OBJECTIVE: This study investigates the therapeutic efficacy of sericin in rats with knee osteoarthritis KOA induced with monosodium iodoacetate MIA, focusing on evaluating its effectiveness through the TGF-βSmad pathway. MATERIALS AND METHODS: The KOA model was established by injecting MIA into the knee joint and the rats were randomly divided into three groups:group 1 control, group 2 KOA control, and group 3 KOAsericin. Sericin was administered intratendinously to rats on days 1,7,14, and 21 50 µL,0.8 gkgmL. After 21 days, the rats were sacrificed, and serum samples were analyzed using the ELISA method to measure TGF-β1, Smad2, and CTGF levels. Additionally, knee joint samples underwent histopathological evaluations with hematoxylin-eosin staining and immunohistochemical assessment using TGF-β1 and Smad23 antibodies. RESULTS: Serum TGF-β1 and CTGF levels were significantly increased in group 2 vs. group 1 P0.05. A statistically significant decrease was observed in group 3 P0.05. Serum Smad2 levels were not significantly different between groups. Histopathologically, group 2 showed a narrowing of the joint space, degeneration of the cartilage, and deep fissures. On the other hand, group 3 showed marked joint space expansion, articular chondral regeneration, and immunostaining patterns similar to group 1. Immunohistochemically, group 2 exhibited a significant increase in TGF-β1 and Smad expression, whereas group 3 significantly decreased these expressions than group 2P0.01. DISCUSSION: Sericin demonstrates potential efficacy in the experimental KOA model in rats through the TGF-β1Smad pathway. Consequently, sericin may emerge as a promising therapeutic agent for the treatment of KOA with further support from advanced clinical trials.

 OBJECTIVE: This study investigates the therapeutic efficacy of sericin in rats with knee osteoarthritis KOA induced with monosodium iodoacetate MIA, focusing on evaluating its effectiveness through the TGF-βSmad pathway. MATERIALS AND METHODS: The KOA model was established by injecting MIA into the knee joint and the rats were randomly divided into three groups:group 1 control, group 2 KOA control, and group 3 KOAsericin. Sericin was administered intratendinously to rats on days 1,7,14, and 21 50 µL,0.8 gkgmL. After 21 days, the rats were sacrificed, and serum samples were analyzed using the ELISA method to measure TGF-β1, Smad2, and CTGF levels. Additionally, knee joint samples underwent histopathological evaluations with hematoxylin-eosin staining and immunohistochemical assessment using TGF-β1 and Smad23 antibodies. RESULTS: Serum TGF-β1 and CTGF levels were significantly increased in group 2 vs. group 1 P0.05. A statistically significant decrease was observed in group 3 P0.05. Serum Smad2 levels were not significantly different between groups. Histopathologically, group 2 showed a narrowing of the joint space, degeneration of the cartilage, and deep fissures. On the other hand, group 3 showed marked joint space expansion, articular chondral regeneration, and immunostaining patterns similar to group 1. Immunohistochemically, group 2 exhibited a significant increase in TGF-β1 and Smad expression, whereas group 3 significantly decreased these expressions than group 2P0.01. DISCUSSION: Sericin demonstrates potential efficacy in the experimental KOA model in rats through the TGF-β1Smad pathway. Consequently, sericin may emerge as a promising therapeutic agent for the treatment of KOA with further support from advanced clinical trials.

 OBJECTIVE: This study investigates the therapeutic efficacy of sericin in rats with knee osteoarthritis KOA induced with monosodium iodoacetate MIA, focusing on evaluating its effectiveness through the TGF-βSmad pathway. MATERIALS AND METHODS: The KOA model was established by injecting MIA into the knee joint and the rats were randomly divided into three groups:group 1 control, group 2 KOA control, and group 3 KOAsericin. Sericin was administered intratendinously to rats on days 1,7,14, and 21 50 µL,0.8 gkgmL. After 21 days, the rats were sacrificed, and serum samples were analyzed using the ELISA method to measure TGF-β1, Smad2, and CTGF levels. Additionally, knee joint samples underwent histopathological evaluations with hematoxylin-eosin staining and immunohistochemical assessment using TGF-β1 and Smad23 antibodies. RESULTS: Serum TGF-β1 and CTGF levels were significantly increased in group 2 vs. group 1 P0.05. A statistically significant decrease was observed in group 3 P0.05. Serum Smad2 levels were not significantly different between groups. Histopathologically, group 2 showed a narrowing of the joint space, degeneration of the cartilage, and deep fissures. On the other hand, group 3 showed marked joint space expansion, articular chondral regeneration, and immunostaining patterns similar to group 1. Immunohistochemically, group 2 exhibited a significant increase in TGF-β1 and Smad expression, whereas group 3 significantly decreased these expressions than group 2P0.01. DISCUSSION: Sericin demonstrates potential efficacy in the experimental KOA model in rats through the TGF-β1Smad pathway. Consequently, sericin may emerge as a promising therapeutic agent for the treatment of KOA with further support from advanced clinical trials.

 AMAÇ: Bu çalışmada; Monosodyum iyoKOAsetat MIA ile diz Osteoartrit KOA modeli oluşturulan sıçanlarda serisinin terapotik etkinliğinin incelenmesi ve bu etkinliğinin TGF-βSmad yolağı üzerinden değerlendirilmesi amaçlanmıştır. MATERYAL VE METOD: Sıçanlarda KOA modeli diz eklemine MIA enjekte edilerek oluşturulmuş ve rastgele 3 gruba ayrılmıştır 1. grup kontrol, 2. grup KOA kontrol, 3. grup KOAserisin. Sıçanlara, serisin 1, 7, 14 ve 21. günlerde 100 µL, 0,8 gkgmL intratendinöz olarak uygulanmıştır. Sıçanlar 21 günün sonunda sakrifiye edilerek elde edilen serum örneklerinde TGF-β1, Smad2, CTGF, seviyeleri ELİSA yöntemi ile belirlenmiştir. Ayrıca diz eklem örneklerinde Hemotoksilen eozin boyası ile histopatolojik, TGF-β1 ve Smad23 antikorları ile immünohistokimyasal değerlendirmeleri gerçekleştirilmiştir. BULGULAR: Serum TGF-β1 ve CTGF düzeylerinde 2. grupda, 1. gruba göre anlamlı olarak anlamlı artış tespit edilmiş P 0.05, tedavi verilen 3. grupta ise istatiksel olarak anlamlı azalma görülmüştür P0.05. Serum Smad2 düzeylerinde gruplar arasında anlamlı fark saptanmamıştır. Histopatolojik olarak 2. grupda subkondral kemik dokusu ve kıkırdak dejenerasyonu ve derin çatlaklar görülmüştür. 3. grup ise kondrosit hücrelerindeki dejenerasyonun azaldığı kıkırdak dokusunun kalınlığının arttığı ve kıkırdak matriksin normale yakın olduğu izlenmiştir. İmmünohistokimyasal olarak 2. grupda TGF-β1 ve Smad ekspresyonlarında artışlar görülmüştür. Bununla birlikte, 3. grup 2. gruba kıyasla bu ekspresyonlarda azalmalar sergilenmiştir. TARTIŞMA: Serisin sıçanlarda deneysel KOA modelinde TGF-β1Smad yolağı üzerinden potansiyel etkinlik göstermektedir. Sonuç olarak, serisin ileri klinik çalışmalarla desteklenmesiyle KOA tedavisi için umut verici bir terapötik ajan olabilir.

Makale detayları

Investigation of The Efficacy of Sericin in Experimental Knee Osteoarthritis Model in Rats through the TGF-Beta/Smad Pathway

Yazarlar:

Koksal Gundogdu | Özgen Kılıç Erkek | Gülşah Gündoğdu | Dilek Sayın | Gülçin Abban Mete

Yayıncı:

Ataturk University

Ana Başlıklar:

Veteriner Anatomi ve Fizyoloji | Veteriner Cerrahi | Veteriner Bilimleri (Diğer)

Dergi:

Veterinary Sciences and Practices Vol.19 Issue 2, pp.85-93

Belge Tipi:

Makale

Yayın Tarihi:

8/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001325986

pISSN:

2822-3608

Sericin

Knee osteoarthritis

TGF-β1/Smad pathwaySerisin

Diz osteoartrit

TGF-β1/Smad yolağı

Sayı

Makale detayları

Investigation of The Efficacy of Sericin in Experimental Knee Osteoarthritis Model in Rats through the TGF-Beta/Smad Pathway

Yazarlar: Koksal Gundogdu | Özgen Kılıç Erkek | Gülşah Gündoğdu | Dilek Sayın | Gülçin Abban Mete

Yayıncı: Ataturk University

Ana Başlıklar: Veteriner Anatomi ve Fizyoloji | Veteriner Cerrahi | Veteriner Bilimleri (Diğer)

Dergi: Veterinary Sciences and Practices Vol.19 Issue 2, pp.85-93

Belge Tipi: Makale

Yayın Tarihi: 8/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001325986

pISSN: 2822-3608

Sericin

Knee osteoarthritis

TGF-β1/Smad pathwaySerisin

Diz osteoartrit

TGF-β1/Smad yolağı

Sayı

Yazarlar:

Koksal Gundogdu | Özgen Kılıç Erkek | Gülşah Gündoğdu | Dilek Sayın | Gülçin Abban Mete

Yayıncı:

Ataturk University

Ana Başlıklar:

Veteriner Anatomi ve Fizyoloji | Veteriner Cerrahi | Veteriner Bilimleri (Diğer)

Dergi:

Veterinary Sciences and Practices Vol.19 Issue 2, pp.85-93

Belge Tipi:

Makale

Yayın Tarihi:

8/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001325986

pISSN:

2822-3608