The  Effect Of Different Workout Programs  On The Expression Of The Genes Related To Oxidative Stress And Immune System

Neşe AKPINAR KOCAKULAK; Serpi TAHERİ; Elif Funda ŞENER; Kenan AYCAN; Yusuf ÖZKUL

doi:https://doi.org/10.38061/idunas.726846

Makale detayları

The Effect Of Different Workout Programs On The Expression Of The Genes Related To Oxidative Stress And Immune System

Yazarlar:

Neşe AKPINAR KOCAKULAK | Serpi TAHERİ | Elif Funda ŞENER | Kenan AYCAN | Yusuf ÖZKUL

Yayıncı:

Izmir University of Democracy

Ana Başlıklar:

Yaşam bilimleri

Dergi:

Natural and Applied Sciences Journal 2020 Vol.3 Issue 1, pp.40-46

Belge Tipi:

Makale

Yayın Tarihi:

6/2020

Belge Dili:

İngilizce

Document ID (Org No.):

2000609411

Sıklık:

İki Yıllık

pISSN:

2645-9000

DOI:

https://doi.org/10.38061/idunas.726846

Özet:

 

Background: exercise conditions can affect the expression of genes. Our study investigated the effects of acute and chronic exercise on the expression of genes related to oxidative stress (Tfam,UCP2,UCP3 ) and immune system (IL-1α,IL-1β,IL-2,IL-8,IL12A,IL12B,TLR2,TLR4 ).
 Methods: was formed with 24 people: 12 healthy females and 12 healthy males. Maximal oxygen use capacities of the participants were determined by the Bruce test protocol at the beginning and end of the 8-week training program. After calculating their maximal oxygen use capacity, each participant was given a acute running exercise on the tread mill at the speed and incline that the participant would reach to his/her maxVO2 until exhausted. The same people was built to  continuous runs (%50-70) for 8 weeks in a week, and two days of medium-term interval training program (%90-95). Peripheral blood samples were taken before and after acute exercise with immediately after chronic exercises. RNA isolation was performed using TRIzol Reagent from peripheral blood mononuclear cells. Gene expression was determined by Biomark Real-Time PCR (RT-PCR). Comparisons were performed by using two independent sample t-test and Mann-Whitney U for quantitative data and calculated gene expression values. The statistical significance level was taken as p <0,05. Results: we found that acute exercise in women changed Tfam gene expression (p <0,05 ). Chronic exercise changes the expression level of more genes (Tfam, IL-1β, TLR4 ) in women (p<0.05,p<0.001). There was only difference in Tfam gene in males (p <0,05 ). Conclusions: changes in gene expression differed by sex in exercise. Our results indicate that different workout programs for females and males cause genes to work and they have a positive effect on their expressions and thus increase the efficiency of the exercise.

Makale detayları

The Effect Of Different Workout Programs On The Expression Of The Genes Related To Oxidative Stress And Immune System

Yazarlar: Neşe AKPINAR KOCAKULAK | Serpi TAHERİ | Elif Funda ŞENER | Kenan AYCAN | Yusuf ÖZKUL

Yayıncı: Izmir University of Democracy

Ana Başlıklar: Yaşam bilimleri

Dergi: Natural and Applied Sciences Journal 2020 Vol.3 Issue 1, pp.40-46

Belge Tipi: Makale

Yayın Tarihi: 6/2020

Belge Dili: İngilizce

Document ID (Org No.): 2000609411

Sıklık: İki Yıllık

pISSN: 2645-9000

DOI: https://doi.org/10.38061/idunas.726846

Sayı

Yazarlar:

Neşe AKPINAR KOCAKULAK | Serpi TAHERİ | Elif Funda ŞENER | Kenan AYCAN | Yusuf ÖZKUL

Yayıncı:

Izmir University of Democracy

Ana Başlıklar:

Yaşam bilimleri

Dergi:

Natural and Applied Sciences Journal 2020 Vol.3 Issue 1, pp.40-46

Belge Tipi:

Makale

Yayın Tarihi:

6/2020

Belge Dili:

İngilizce

Document ID (Org No.):

2000609411

Sıklık:

İki Yıllık

pISSN:

2645-9000

DOI:

https://doi.org/10.38061/idunas.726846