Influence of Carbon Source, Nitrogen and PEG on Caffeic Acid Derivatives Production in Callus Cultures of Echinacea purpurea L.

Münüre Tanur Erkoyuncu; Mustafa Yorgancılar

Makale detayları

Influence of Carbon Source, Nitrogen and PEG on Caffeic Acid Derivatives Production in Callus Cultures of Echinacea purpurea L.

Yazarlar:

Münüre Tanur Erkoyuncu | Mustafa Yorgancılar

Yayıncı:

Tekirdag Namik Kemal University

Ana Başlıklar:

Tarımda Bitki Islahı

Dergi:

Tekirdağ Ziraat Fakültesi Dergisi Vol.21 Issue 1, pp.35-45

Belge Tipi:

Makale

Yayın Tarihi:

1/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001310398

pISSN:

1302-7050

Anahtar kelimeler:

Özet:

 This study aimed to determine the effects of polyethylene glycol PEG as an abiotic elicitor and nutritional factors different ammoniumnitrate ratios, carbon source and amount in the culture medium on the production of Caffeic Acid Derivatives CADs in callus cultures of Echinacea purpurea L. Petiole and root explants were cultured on MS medium modified in terms of different types sucrose and maltose and amounts sucrose 15, 45, 60 g l-1, and maltose 15, 30, 45, 60 g l-1 of carbon source, different concentrations 5, 10, 15 g l-1 of PEG and ammonium nitrate ratios 0:35, 5:25, 15:15, 35:0 mM. The amounts of CADs in the callus obtained at the end of the 10-week culture period were analysed. In both explant types, the highest amount of CADs were obtained from the medium containing 15 g l-1 sucrose and 15 or 30 g l-1 maltose applications, while the highest amount of CADs was obtained in the medium containing 0:35 mM ammoniumnitrate in nitrogen applications. While the highest amount of CADs in root explant was obtained from the medium containing 10 g l-1 PEG applications, CADs content could not be obtained in petiole explant. As a result, the highest amounts of caftaric, chlorogenic, caffeic, and chicoric acids respectively, 9.38, 0.71, 0.29, and 34.77 mg g-1 were determined at callus obtained from root explant cultured on MS medium containing 30 g l-1 sucrose and 0:35 mM ammoniumnitrate. In conclusion, optimization of culture conditions and different elicitor applications were made to increase secondary metabolite content in E. purpurea L. under in vitro conditions and the results obtained were presented comparatively.

 This study aimed to determine the effects of polyethylene glycol PEG as an abiotic elicitor and nutritional factors different ammoniumnitrate ratios, carbon source and amount in the culture medium on the production of Caffeic Acid Derivatives CADs in callus cultures of Echinacea purpurea L. Petiole and root explants were cultured on MS medium modified in terms of different types sucrose and maltose and amounts sucrose 15, 45, 60 g l-1, and maltose 15, 30, 45, 60 g l-1 of carbon source, different concentrations 5, 10, 15 g l-1 of PEG and ammonium nitrate ratios 0:35, 5:25, 15:15, 35:0 mM. The amounts of CADs in the callus obtained at the end of the 10-week culture period were analysed. In both explant types, the highest amount of CADs were obtained from the medium containing 15 g l-1 sucrose and 15 or 30 g l-1 maltose applications, while the highest amount of CADs was obtained in the medium containing 0:35 mM ammoniumnitrate in nitrogen applications. While the highest amount of CADs in root explant was obtained from the medium containing 10 g l-1 PEG applications, CADs content could not be obtained in petiole explant. As a result, the highest amounts of caftaric, chlorogenic, caffeic, and chicoric acids respectively, 9.38, 0.71, 0.29, and 34.77 mg g-1 were determined at callus obtained from root explant cultured on MS medium containing 30 g l-1 sucrose and 0:35 mM ammoniumnitrate. In conclusion, optimization of culture conditions and different elicitor applications were made to increase secondary metabolite content in E. purpurea L. under in vitro conditions and the results obtained were presented comparatively.

 This study aimed to determine the effects of polyethylene glycol PEG as an abiotic elicitor and nutritional factors different ammoniumnitrate ratios, carbon source and amount in the culture medium on the production of Caffeic Acid Derivatives CADs in callus cultures of Echinacea purpurea L. Petiole and root explants were cultured on MS medium modified in terms of different types sucrose and maltose and amounts sucrose 15, 45, 60 g l-1, and maltose 15, 30, 45, 60 g l-1 of carbon source, different concentrations 5, 10, 15 g l-1 of PEG and ammonium nitrate ratios 0:35, 5:25, 15:15, 35:0 mM. The amounts of CADs in the callus obtained at the end of the 10-week culture period were analysed. In both explant types, the highest amount of CADs were obtained from the medium containing 15 g l-1 sucrose and 15 or 30 g l-1 maltose applications, while the highest amount of CADs was obtained in the medium containing 0:35 mM ammoniumnitrate in nitrogen applications. While the highest amount of CADs in root explant was obtained from the medium containing 10 g l-1 PEG applications, CADs content could not be obtained in petiole explant. As a result, the highest amounts of caftaric, chlorogenic, caffeic, and chicoric acids respectively, 9.38, 0.71, 0.29, and 34.77 mg g-1 were determined at callus obtained from root explant cultured on MS medium containing 30 g l-1 sucrose and 0:35 mM ammoniumnitrate. In conclusion, optimization of culture conditions and different elicitor applications were made to increase secondary metabolite content in E. purpurea L. under in vitro conditions and the results obtained were presented comparatively.

 Bu çalışmada, abiyotik bir elisitör olarak polietilen glikolün PEG ve besin ortamındaki farklı beslenme faktörlerinin farklı amonyumnitrat oranları, karbon kaynağı ve miktarı Echinacea purpurea L. kallus kültürlerinde kafeik asit türevlerinin miktarlarına etkisinin belirlenmesi amaçlanmıştır. Petiyol ve kök eksplantları karbon kaynağının farklı türleri sakkaroz ve maltoz ve miktarları sakkaroz 15, 45, 60 g l-1 ve maltoz 15, 30, 45, 60 g l-1, farklı amonyum nitrat oranları 0:35, 5:25, 15:15, 35:0 mM ve farklı konsantrasyonlarda 5, 10, 15 g l-1 PEG içeren MS besin ortamlarında 10 hafta boyunca kültür alınmış ve kültür süresi sonunda elde edilen kalluslarda kaftarik, klorojenik, kafeik ve kikorik asit miktarları tespit edilmiştir. Her iki eksplant türünde de en yüksek kafeik asit türevlerinin miktarları, karbon kaynağı uygulamalarında, 15 g l-1 sakkaroz ile 15 ve 30 g l-1 maltoz; nitrojen uygulamalarında ise 0:35 mM amonyumnitrat içeren besin ortamlarında uyarılan kalluslarda tespit edilmiştir. PEG uygulamalarında ise, en yüksek miktarlarda kafeik asit türevleri, 10 g l-1 PEG içeren besin ortamından kök eksplantından uyarılan kalluslarda elde edilirken, petiyol eksplantından elde edilen kallus dokularında kafeik asit türevlerinden hiçbiri tespit edilememiştir. E. purpurea L kallus kültürlerinde, kafeik asit türevlerinin miktarını artırmak için, in vitro şartlar altında, abiyotik elisitör olarak PEG ve farklı beslenme faktörlerinin optimizasyonu araştırıldığı bu çalışmada, elde edilen sonuçlar karşılaştırmalı olarak sunulmuştur. Tüm uygulamalar değerlendirildiğinde; sonuç olarak, en yüksek kaftarik, klorojenik, kafeik ve kikorik asit miktarları sırasıyla 9.38, 0.71, 0.29 ve 34.77 mg g-1 0:35 mM amonyumnitrat ve 30 g l-1 sakkaroz içeren MS ortamında kültüre alınan kök eksplantından elde edilen kallus dokularında tespit edilmiştir.

Makale detayları

Influence of Carbon Source, Nitrogen and PEG on Caffeic Acid Derivatives Production in Callus Cultures of Echinacea purpurea L.

Yazarlar:

Münüre Tanur Erkoyuncu | Mustafa Yorgancılar

Yayıncı:

Tekirdag Namik Kemal University

Ana Başlıklar:

Tarımda Bitki Islahı

Dergi:

Tekirdağ Ziraat Fakültesi Dergisi Vol.21 Issue 1, pp.35-45

Belge Tipi:

Makale

Yayın Tarihi:

1/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001310398

pISSN:

1302-7050

Sucrose

Maltose

Nitrogen

PEG

Caffeic acid derivatives

Echinacea purpurea L.

HPLCSakkaroz

Maltoz

Azot

PEG

Kafeik asit türevleri

Echinacea purpurea L.

HPLC

Sayı

Makale detayları

Yazarlar: Münüre Tanur Erkoyuncu | Mustafa Yorgancılar

Yayıncı: Tekirdag Namik Kemal University

Ana Başlıklar: Tarımda Bitki Islahı

Dergi: Tekirdağ Ziraat Fakültesi Dergisi Vol.21 Issue 1, pp.35-45

Belge Tipi: Makale

Yayın Tarihi: 1/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001310398

pISSN: 1302-7050

Sayı

Yazarlar:

Münüre Tanur Erkoyuncu | Mustafa Yorgancılar

Yayıncı:

Tekirdag Namik Kemal University

Ana Başlıklar:

Tarımda Bitki Islahı

Dergi:

Tekirdağ Ziraat Fakültesi Dergisi Vol.21 Issue 1, pp.35-45

Belge Tipi:

Makale

Yayın Tarihi:

1/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001310398

pISSN:

1302-7050